Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,331,496	C→A	intergenic (‑1699/+2840)	yfaQ ← / ← yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,331,496	0	C	A	100.0%	52.4 / NA	17	intergenic (‑1699/+2840)	yfaQ/yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/7);  total (10/7)

TACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTCTGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGGAAC  >  NC_000913/2331261‑2331736                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 tactTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATACTTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAgcg                                                                                                                                                                                                                                   >  2:76039/1‑251 (MQ=255)           ccGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAAtt                                                                                                                                                                                                                         <  1:301258/251‑1 (MQ=255)                                                       caccacCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACCGTTTTGTGCTACTGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGAt                                                                                                                                                                              >  2:15864/1‑250 (MQ=255)                                                            ccGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAg                                                                                                                                                                        <  1:2847/251‑1 (MQ=255)                                                                     aCATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTgccgc                                                                                                                                                               >  1:31199/1‑251 (MQ=255)                                                                                                                  aGTTTTGTGCTAATGCTGTCCTGCACTTGCACATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCAt                                                                                                                  >  1:249769/1‑251 (MQ=255)                                                                                                                        gtgCTAATGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTaga                                                                                                             <  1:287121/250‑1 (MQ=255)                                                                                                                                                                                                tGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTc                                                     <  1:301214/234‑1 (MQ=255)                                                                                                                                                                                                tGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTc                                                     >  2:301214/1‑234 (MQ=255)                                                                                                                                                                                                    tCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCAACGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGAAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGc                                >  2:128922/1‑251 (MQ=255)                                                                                                                                                                                                    acAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCAACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCATGCCAGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGCTACGc                                >  1:12596/2‑251 (MQ=255)                                                                                                                                                                                                         gAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCg                           >  2:300411/1‑251 (MQ=255)                                                                                                                                                                                                                    cGCCCCTTTGTGCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAAt                <  1:300411/251‑1 (MQ=255)                                                                                                                                                                                                                      caccTTTGTGCAGCGTCAGCGTATGGCGCAGCTCAGCGCCTGCAAATTTAGTCTCCAGCGTCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGCCCTGCCTCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCa              <  1:136835/249‑1 (MQ=255)                                                                                                                                                                                                                            tgtgCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGc                                                                                    >  2:154396/1‑175 (MQ=255)                                                                                                                                                                                                                            tgtgCAGCGTCAGCGTATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGc                                                                                    <  1:154396/175‑1 (MQ=255)                                                                                                                                                                                                                                cagcgtcagcgtATGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAgg     >  2:151828/1‑250 (MQ=255)                                                                                                                                                                                                                                  gcggcagcgtaTGGCGCATCTCAGCGCCTGCAAAGTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGGAAc  <  2:87740/247‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 TACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGTCCTGCACTTGCCCATTTTGTTGCAGTTCTGCACTTAACAAGCCAGATTGCTGAATATTCTGCGTCAGCGAAATATAATTCGCCCCTTTGTGCAGCGTCAGCGTCTGGCGCATCTCAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGGAAC  >  NC_000913/2331261‑2331736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑