Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,083	T→C	T239A (ACG→GCG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,083	0	T	C	95.7%	70.2 / ‑6.9	23	T239A (ACG→GCG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (7/15);  total (7/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATC  >  NC_000913/2328846‑2329329                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       gCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACggg                                                                                                                                                                                                                                             <  1:250855/249‑1 (MQ=255)  cAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCAGTAGTAAACGTCACACGATAGCGCCCATTAAGTGCGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTTAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCGGCGTCGGCACGGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTa                                                                                                                                                                                                                                          <  1:184522/251‑1 (MQ=255)        cAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCg                                                                                                                                                                                                                                    <  1:40802/251‑1 (MQ=255)                           aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCCCCTCTACAC‑ATTGCCCCCTGGCAACCGGCGTCGGCACTGGCAAACCCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGcc                                                                                                                                                                                                                 <  2:68145/251‑1 (MQ=255)                           aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGcc                                                                                                                                                                                                                 >  1:274948/1‑251 (MQ=255)                                   cgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAAccc                                                                                                                                                                                                         <  1:313193/251‑1 (MQ=255)                                        cACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAg                                                                                                                                                                                                     <  1:131118/250‑1 (MQ=255)                                         aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCCCAGCg                                                                                                                                                                                                   >  1:106232/1‑251 (MQ=255)                                                  gACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTTCTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCTCCTCTACCC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCAt                                                                                                                                                                                          <  1:231707/251‑1 (MQ=255)                                                                    ccctttAGTAACCGTCACACGATATCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTCCCGCTGTGATTTTCTTCCGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCaa                                                                                                                                                                        <  2:221800/247‑1 (MQ=255)                                                                           gTAAACGTCACCCGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGCCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAACAAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAaccac                                                                                                                                                                  <  2:226836/250‑1 (MQ=255)                                                                             aaaCGGCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCCCTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCCCAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCAccc                                                                                                                                                                <  1:306252/250‑1 (MQ=255)                                                                                cGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGAATAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCAAAGCGGTGTACCATCGGGTAACCAGCCACCACAAACACCCTGAc                                                                                                                                                            >  2:169439/1‑251 (MQ=255)                                                                                         aTAGCGCTCATTAAGTACGCCGCGATTTACTGCCGTCGTACCCTTTCCCGCTGTGATTTTCTTCGGCGGATATCGGGCAAACAGCTCCACCTCCCCACTATT‑CCCCCTGGCAACCTGCGTCTGCACTGGAAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTTCCATTGCCAGTAACCCACAGCGGTGTACCATCGGTTAAGCAGCCAGCAAAACCACCCTGACGGAttttct                                                                                                                                                   <  2:52791/251‑4 (MQ=255)                                                                                                  aTTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTCCAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCAACGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACAGCCAGTAACCCAAAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACAACCCTGAAGGATTTCCTGTTTatcat                                                                                                                                          >  1:301648/1‑251 (MQ=255)                                                                                                               ggATTTACTGCCGTCTGAATCTTTTCCGCGTTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAGCCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCGCCCTGACGGAGTTCCTGTTTATCATCGGCAAACCAGCt                                                                                                                             <  2:131624/251‑1 (MQ=255)                                                                                                                                                            gaaTAGCGGGCAAACAGCTCCCCCTCTACCC‑AGTTCCCACTGGCACCCTGCGTCGGCATTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGGACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCTGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGAACCCGACTACCGAGCATGGATGCGACAcc                                                                                <  2:18130/249‑1 (MQ=255)                                                                                                                                                                                         cc‑aTTGCCCACTGGCAACCGGCGGCGGCACTGGCAAAACCCGATTAAGCACCGCCTCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGGAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGGACCCGACTACCTAGCATGGATGCGACACCGATTTTCGCCGCGTCCAGCACCACATCCa                                                   <  2:130283/250‑1 (MQ=255)                                                                                                                                                                                             ttGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGAACCGTTTTGCTGGTGACACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACAACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGATTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGt                                                 >  1:302636/1‑250 (MQ=255)                                                                                                                                                                                                                         ggCAAAACCCGATTAAGCACCGCCGCATAACGGGTTAGTACCGTTTGGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCTTCGGTTAACCAGCCAGCAAAACCTCCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCTACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGATCTTTGTTTTGCGCgg                     <  2:274948/250‑1 (MQ=255)                                                                                                                                                                                                                                       aaGCCCCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGATGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTGTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATg      <  1:315007/251‑1 (MQ=255)                                                                                                                                                                                                                                        aGCACCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATg      >  1:119057/1‑250 (MQ=255)                                                                                                                                                                                                                                           aCCGCCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATc  >  1:154606/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACAC‑ATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATC  >  NC_000913/2328846‑2329329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑