Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	53.2 / NA	16	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

AGATTAAGGTGCGGAACAGATTTCCACG‑ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACG  >  NC_000913/2173202‑2173670                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               ataTTAAGGTGCGGAAAAGATTTCC‑CGTATGCACGCCGACCTCCATCGCCACGAAGAATCCGATGGTGGAAAGATCGCCAAACGACAGCTCCTGATTACCCGGCACACAAACACCAATTAAAATGGTAAGTGGTATAAAAATCAGGCTGTCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGTCCAATCAGGAACGCCTGACCGCCGAACTTCGCCTGTAAACGAATACGAGCTTGCTTa                                                                                                                                                                                                                          <  1:183167/249‑1 (MQ=255)            gggAACAGATTCCCGCG‑ATGATCGGCACCCGCCTTCGCCCCGAAGAAGCCGATGTGGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTAAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGt                                                                                                                                                                                                               <  1:131414/250‑1 (MQ=255)                                 cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACACGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt                                                                                                                                                                                           >  2:156196/1‑251 (MQ=255)                                         cccccATCCCCACGAAGAAGCCGATGGTGGCAAGACCGCCAAACGGCAGCACCGAATTCCCCGGCACACAAACAGCAAATAAAATGGTGAGTGGAATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc                                                                                                                                                                                   <  1:53817/248‑1 (MQ=255)                                                      aaaaaagCGGATGGGGGCAGGACCGCCAAAGGGCAGCACCTGATTACCCGGAACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAACCAGGCTTGCGGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagca                                                                                                                                                                      <  2:264444/247‑1 (MQ=255)                                                                      gaaaGATCGCAAAACGGCAGCCCCTGATTCCCCGGCACACAAACAGCATTTAAAAGGGTGAGGGGAATAAAAATCGGTGTTGCCGATAC‑‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGt                                                                                                                                                      <  1:310717/249‑1 (MQ=255)                                                                            ccGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAAAAAAACCAGGCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAc                                                                                                                                                <  1:41406/250‑1 (MQ=255)                                                                                                                         aaaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGcc                                                                                                   >  1:273109/1‑251 (MQ=255)                                                                                                                                  aGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                                                                            >  1:2104/1‑249 (MQ=255)                                                                                                                                                                        gTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACg                                                                       >  2:212216/1‑234 (MQ=255)                                                                                                                                                                        gTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACg                                                                       <  1:212216/234‑1 (MQ=255)                                                                                                                                                                                               aTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAatat                               >  1:228689/1‑251 (MQ=255)                                                                                                                                                                                                aaaaGGCCAATCAGGAACGCCGGACGGCCGAACGTCGCATGTAAACGACGACGAGCTTCCTTAGCGATGGGCGTTAAACCATCCACGAAGGGTTTAATCACCCGTGGCATTAGCAGCAACGCTGCCGCCGGTTTTAGCGCCGGCTGCATTACTCCTTTGACATCGTAACCCGCGAGGATGCGGATAATCAGCGCCAGCGCAAAACCCACTGATACAGGCTGGCCAAATGGACCAAATTTGCGCTGAATAtc                              <  1:293423/249‑1 (MQ=255)                                                                                                                                                                                                   aGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACATGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgtc                           <  1:156196/251‑1 (MQ=255)                                                                                                                                                                                                        aaTCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAATACGAGCTTGCTTAGCGATGGGCGATAAACCATCCATGATGGGTTTAATCACCCGTGCCATTAGCAGCATCACTGCCGCCGTTTTGACAGCCAGCTGCAATACACCTTTGACCTCGTAACCCGCGAGAATGACGATAATCAGACCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGATGAATATCGTCGgcgc                      >  1:219383/1‑251 (MQ=255)                                                                                                                                                                                                                            cgAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACg  >  1:101539/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               AGATTAAGGTGCGGAACAGATTTCCACG‑ATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACG  >  NC_000913/2173202‑2173670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑