Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,042,171	G→A	intergenic (+146/‑197)	zinT → / → yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,042,171	0	G	A	100.0%	55.9 / NA	16	intergenic (+146/‑197)	zinT/yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

TGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTAC  >  NC_000913/2041952‑2042401                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       tgGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAAt                                                                                                                                                                                                          >  1:24217/1‑250 (MQ=255)    aaaaCTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCACGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACgg                                                                                                                                                                                                      >  1:262761/1‑251 (MQ=255)        cTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCa                                                                                                                                                                                                   >  2:7045/1‑250 (MQ=255)                                               agTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGTAAGATACCTGTGCTCACGCCATTGCTTATATTg                                                                                                                                                           >  1:192870/1‑251 (MQ=255)                                                         gAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACa                                                                                                                                                 <  1:235136/251‑1 (MQ=255)                                                                      aTTGAGGATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTAACATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTAGGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATGGCTTATATTGGCTGGTTACAATATGCTCTATCa                                                                                                                                    <  2:203029/251‑1 (MQ=255)                                                                           gTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAAtttt                                                                                                                               >  1:253953/1‑251 (MQ=255)                                                                                               cGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTaaa                                                                                                           <  1:181103/251‑1 (MQ=255)                                                                                                                     cATAACTGCCACTGGTCCATCCACAAACGCCCCTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATCCGGCCAACAGAGAAAGATACCTGTGCTCACGCCAtt                                                                                                                                                                     >  1:128084/1‑171 (MQ=255)                                                                                                                                           acaATCGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCGCTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAATATACCTGGGCTCACGCCATTGCGTAGATTGGCTGTTTACAATATGCACTATTAATGTTTTAAATAACAAATTATGAAAAGCTGGTTGGTATAAGGAATTTTTAAGGTGCGTGGGATAGCTTg                                                               <  1:29165/251‑1 (MQ=255)                                                                                                                                                      ggAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGGCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCGCGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGGGAAAAt                                                    <  2:115428/250‑1 (MQ=255)                                                                                                                                                                        aGACACGATCATCAATATGGCGTGTCTGTATATTCATAAGCTACCCCGCATTAAGTTAACCACTAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCAAGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCCCAGGAACTACAAAAAt                                  >  2:39130/1‑251 (MQ=255)                                                                                                                                                                          acacGCTCATCACTATTACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGa                                <  1:183437/251‑1 (MQ=255)                                                                                                                                                                                                         taataaGCTAACCCGCAATAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGATAAAGATTCCTTTGCTCACTCCATTGCTTATTTTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATGTTTAAGGTTCGTGGGATAGCGTGACTGTGAAAATCACATGAGCGACAAAAATGAACCGAGTCTCAAAAACTCAAATTTATTTAc  <  2:312729/250‑1 (MQ=255)                                                                                                                                                                                                                cTAACCCGCATTAAGTTAACCAATAACGGCTTCCATACACAATACGGCCAAAAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAACTAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCg                            >  2:246519/1‑217 (MQ=255)                                                                                                                                                                                                                cTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCg                            <  1:246519/217‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                       TGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTAC  >  NC_000913/2041952‑2042401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑