| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,022,364 | C→T | P166S (CCG→TCG) | fliP → | flagellar biosynthesis protein FliP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,022,364 | 0 | C | T | 95.0% | 63.9 / NA | 20 | P166S (CCG→TCG) | fliP | flagellar biosynthesis protein FliP |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/8); minor base G (0/1); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTGCTGGGGCTGGCACTGTTTTTGACCTTTTTTATTATGTCACCGGTGATCGACAAAATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTT > NC_000913/2022132‑2022596 | aTTGCTGTGGCTGGCACTGTTTTTGGCCGTTTTTATTATGTCACCGGTGATCGACAAAATTTATGTAGATGCGTATCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTc < 2:77187/250‑1 (MQ=255) tttATTATGTCACCGGTGATCGACAAAATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCCGCGAGTTGACAACCGc > 2:121930/1‑251 (MQ=255) tttATTATGTCACCGGTGATCGACAAAATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCg < 1:147987/250‑1 (MQ=255) atGTCACCGGTGATCGACAAAATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTc < 1:218529/250‑1 (MQ=255) gATCGACAAAATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATGTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCAGGGCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCa < 1:44747/251‑1 (MQ=255) gCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGAcc > 1:65264/1‑251 (MQ=255) agaAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTg < 2:264212/227‑1 (MQ=255) agaAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTg > 1:264212/1‑227 (MQ=255) aTGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGc > 1:98082/1‑251 (MQ=255) aggaggCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTATTTGCCAGCCTGGCGAATACCGGCCCGTTGCCGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCGGCGAGTTGAAAACCGCATTTAAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGAGTGTTGATGGCATTg > 2:233542/1‑251 (MQ=255) tGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCAtt > 1:232517/1‑251 (MQ=255) ggCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTAc > 1:133600/1‑251 (MQ=255) gATTTAGGGTTGTTTGCCAGACTGGCGACTACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTTGGGATGATGCTGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAACCTGATGCTGTTTGTACtggg > 2:104755/1‑250 (MQ=255) ttGCCAGACTGGCGACTACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCa > 1:66229/1‑250 (MQ=255) gCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCTCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAAtt < 1:233542/251‑1 (MQ=255) caaaccggCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGGGTCGATGCGCGTTTTGCTCCCGGCCTACGGGACCGGCGGGTTGCCAACCGCATTACAGGGATGATTCCCGCTTTTCATCCCTTTTTTGATTATCTACCTGGTGTGAGCAAGAGTGGTGGTGGCATTGGGAATGATGCTGGTTCCCCCAGCCACCATTCCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGCTGGCTAGCTATTGc > 2:237766/7‑251 (MQ=255) ggCCCGTTGCAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCTCCATTGCTCTGCCCTTTAAACTGATGCTGTTGGGACTTGTGTATGGCTGGCAATTGCGGGTCGGTTCGCTGgc < 2:133600/251‑1 (MQ=255) ccGTTGCAGGGACCTGAAGCCGTGGCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCAGCCCTTTTTTGATTATCGACCGGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCGGCCCTTTAAACTGATTGTGTGTGGACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCa < 1:134095/251‑1 (MQ=255) ggtGCAGAGACCTCACGCCGTGTCGATGAGCACTTTCCTCCCGGCCTACGTGACCACAGACTTAAAAACCGCATTTCAGCCAGGCTTCACGATTTTCATCCCTTTTTTGATGACCCAACTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCACCAGCCACCATTGCTCTGCCCTTTAAACTGCTGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTCCGCTggagacgg > 2:184808/3‑245 (MQ=255) cAGGGACCTGAAGCCGTGTCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCtt < 1:104755/251‑1 (MQ=255) | ATTGCTGGGGCTGGCACTGTTTTTGACCTTTTTTATTATGTCACCGGTGATCGACAAAATTTATGTAGATGCGTACCAGCCATTCAGCGAAGAGAAAATATCAATGCAGGAGGCGCTGGAAAAAGGGGCGCAGCCGCTGCGTGAGTTTATGCTGCGTCAGACCCGTGAGGCAGATTTAGGGTTGTTTGCCAGACTGGCGAATACCGGCCCGTTGCAGGGACCTGAAGCCGTGCCGATGCGCATTTTGCTCCCGGCCTACGTGACCAGCGAGTTGAAAACCGCATTTCAGATAGGCTTCACGATTTTCATCCCTTTTTTGATTATCGACCTGGTGATAGCCAGCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTT > NC_000913/2022132‑2022596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |