| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 192,701 | T→C | intergenic (+121/‑171) | pyrH → / → frr | UMP kinase/ribosome‑recycling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 192,701 | 0 | T | C | 100.0% | 45.6 / NA | 18 | intergenic (+121/‑171) | pyrH/frr | UMP kinase/ribosome‑recycling factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/8); total (10/8) | |||||||||||
TCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGC > NC_000913/192468‑192946 | tCACGCTGGCTCGTGACCATAAATTTCCGATTCGTGTTGTCAATATGAACAAACCTGGTGCGCTTCGCCGTGTGGTAATGTGTGAAAAAGAAGGGACTTTAAT‑CACGGAATAAGTCCCGGGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGTTTATAAGGCGTTATTGTTGCATGCAGTTTGGTCACGGCCAGCGCGCAGCGACCTGAGCGCACAAAAGTACGGGAgg < 2:296876/250‑1 (MQ=255) ggCTCGGGACCATAAATTGCCGAGTCGTGTGGTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTGAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTAGTGTTGCAGGCAGTTTGGTCACGGACAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGAtttcg < 2:249770/249‑5 (MQ=255) tACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAGGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCaa > 2:225402/1‑250 (MQ=255) cGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATg < 2:68746/251‑1 (MQ=255) gttgtaactatGAACAAAACGGGTGCGATGCGCCGTGAGGTAATGGGTGAAAAAGAAGGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAACTAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGATATTGTTGCAGGCCGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTCCGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCaacta > 2:125635/9‑247 (MQ=255) cAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTaataat < 1:216523/251‑1 (MQ=255) aagaagGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCtat > 1:29934/1‑250 (MQ=255) agaagGGACGTTAATGCA‑GGAATTAATACCGTGATGGAGTAAATA‑GTGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGGCGGGTTATAAGGCGGCCGTGTTGCAGGCAGTGTGGTCTCGGCCAGCGCTCAGGAACCGGAGCGCACAAAAGTACGTTAGTATGGCGAGCGTTGCCCCGGACCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTACTCGGGGTTATAc < 2:3356/251‑1 (MQ=255) gaagGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGGGGATGGCGAGCACTGCCCGGGGCCACAATGGCAAATAAAATAGCCTAATAATCCAAACGATTACACGTAATATGTTTAATCAGGACTATACt > 2:300888/1‑251 (MQ=255) aT‑CACGGAATAAGTCACGTGATGGA‑TAAATAAGGGGAAGATTCCGTGGAAGTATCGCGGGGGCTGGAGTCTGGTTATAAGGCGTTACTCGTGCAGGCAGTGTGGTCACGGCCAGCGCGGAGCAACCGGAGCGCACAAAAGGACGTGAGGATAGCTCGCACTGCCCGTGGCCAAAAGGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGGAAGATGTTTAATCCGGGCTAGACtttgaactaatcc < 2:71640/251‑12 (MQ=255) tGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGAtt < 2:234905/188‑1 (MQ=255) tGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGAtt > 1:234905/1‑188 (MQ=255) tGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACCGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGACTgtctgttc > 1:208195/1‑248 (MQ=255) tATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCACGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAACGTACGTGCGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGCCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTa > 2:26926/1‑250 (MQ=255) ggCGTAAGTCTGGTTATAAGGCGTTATTGTTGCCGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGCTTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGCCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTCACGTGAtt > 1:94529/1‑249 (MQ=255) gCCAGCGCGCAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCg > 2:225179/1‑251 (MQ=255) gcgcAGCAACCGGAGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGACTTTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCTATATCAGAAAAGATGCTTAAGTACGCATGGACAAATGCGTATAAGTGTTCaaa < 2:161201/251‑1 (MQ=255) aGCGCACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGc > 1:233062/1‑250 (MQ=255) | TCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGGTGCGCTGCGCCGTGTGGTAATGGGTGAAAAAGAAGGGACTTTAAT‑CACGGAATAATTCCCGTGATGGA‑TAAATAAGGGTAAGATTCCGCGTAAGTATCGCGGGGGCGTAAGTCTGGTTATAAGGCGTTATTGTTGCAGGCAGTTTGGTCACGGCCAGCGCGCAGCAACCGGAGCGTACAAAAGTACGTGAGGATGGCGAGCACTGCCCGGGGCCAAAATGGCAAATAAAATAGCCTAATAATCCAGACGATTACCCGTAATATGTTTAATCAGGGCTATACTTAGCACACTTCCACTGTGTGTGACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGC > NC_000913/192468‑192946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |