| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,001,675 | C→A | V39L (GTG→TTG) | fliA ← | RNA polymerase sigma factor FliA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,001,675 | 0 | C | A | 95.7% | 68.6 / NA | 23 | V39L (GTG→TTG) | fliA | RNA polymerase sigma factor FliA |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (6/16); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTC‑ATCCAGCATAGCGCCACGGATACGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACA > NC_000913/2001429‑2001895 | gtaacgcAGTTTCCATGGAGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGAGCAACGTCACGCGCGGTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGGTC‑ATCCAGCATAGCGCCACGGATACGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGACATAGCGTTCGGCGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCACGTTCCACgatc > 2:28037/7‑248 (MQ=255) aCCTCAGTTTTCGTGGAGTTGCGGCCAAGTTACAGCTCCAGTTGCACTATTGCCTGTGACACGTCAAGCGCGTTGCGTCGCACGATGCGCGGCACCCAGTCACGGCTGCGAAGTTC‑ATCCAGCATAGCGCCACGGATACGCTGCACTGCGTAATGTGTAAATGCCGTTCCTTGA‑AGGGCGTCATAGCGCTCGACGGCATTAAGTAACCCAC‑TGAA‑GCCCGCCAGTCACAGATCGTCAAGCTCCAAGCtacc > 2:102862/1‑248 (MQ=255) ccTCAGTTTCCGTGGCGTTGCGGCCAATTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTC‑ATCCAGCATAGCGCCCCGGATACGCTGCCCTGCGTAAGTTGTAAATGCCGTTCCTTGT‑GGGGCGTCATAGCGTTCGACGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTcgcg < 1:79065/251‑1 (MQ=255) ccAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTC‑ATCCAGCATAGCGCCACGGATACGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCCGTCGAACCTGCAGGCGCa > 2:55608/1‑250 (MQ=255) cccTGCTCCGGTTGCCCTATTGCCGGTGCCCTTTCGCGCGCGTTGCGTCGCCCCCGCCGCGGCCCCCAGTCGGGGCTGCGA‑GTTCCAACCAGCTTGGCGCCGCGGATCCGCTTCGGTGCTTAAGT‑GGAAATGCCCTTTCTGGTTAGGGTGTCATACAGTCGGTCGGCATTAAG‑AACCCAAATGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCGCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTc < 1:260565/250‑1 (MQ=255) gCCTGTGCCACTTCACGCGAGTTGCGTCGAACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTC‑ATCCAGCATAGCGCCACGGATACGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGTCATAGCGTTCGCCGGCATTAAGAAACCCAA‑TGCC‑GCCCGCCTGTAGCCGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGAGACGCACCAGCGGGACAt > 2:21274/1‑250 (MQ=255) cccccTTCACGCGCTTTGCGTCGCACGCTGCGCGGCCCCCAGTCCCGGCTGCGATGTTC‑ATCCAGCATAGCGCCCCGGATACGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGTCATAGCTTTCGACGGCATAAAGTACCCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTg < 1:232006/247‑1 (MQ=255) gcgGCACCCAGCCACGGCGGCGAAGTTC‑ACCCAGCATACCGCCACGGATCCGCTGCACTGCGTAAGTTGTAAAGGCCGTTCCGTGT‑GGGGCGTCAGAGCGTTCTACGGCATAAAGGACCCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCGCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCa < 1:314686/251‑1 (MQ=255) aGTTA‑ATCCAACATAGCCCCACGGATCCTCTGCACGGCTTAAGTTGAAAATGCCGTCCCTGGT‑AGGGCTTCATGGCGTTCGCCGGCATAAAGTAACCAAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCTGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAt < 1:122147/251‑1 (MQ=255) tcacccAGCTTAGCCCCAGGGATACGCTGCACTGGGTAATTTGAAAAGGCCGTTCCTTTT‑AGGGCGTAATGGCGTTCGACGGCATAAAG‑AACCCAAATCCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaa < 1:129197/246‑1 (MQ=255) ccAGCATGGCGCCACGGATACGCGGCACTGCGTAAGTTGTAAATGCCGTTCCTGTT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGc < 1:55608/251‑1 (MQ=255) cAGCATAGCGCCACGGATACGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGcc > 1:67266/1‑251 (MQ=255) gggttACGCTGCCCTGCGTAAGTTGTAAATGCCGTTCGTTGT‑GGGGCGTCATAGCGTTCGACGGAATTAAGAACCCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCTCCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa < 1:43180/247‑1 (MQ=255) cGGATACGCTGCACTGCGTAAGTTGAAAAGGCCTTTCCTTGT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTAACCCAA‑GGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa < 1:311113/251‑1 (MQ=255) ccGCTGCACGGCGTAAGTTGTAATTGCCGTTCCTTGT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTACCCCAA‑GGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATAAGTTAt < 1:88439/250‑1 (MQ=255) aCGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTAAACCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCAAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACCGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt > 1:203242/1‑251 (MQ=255) ggccttggtaaaGTTGTAAATCCTGTGCCTTGT‑GGGGCGTCATAGGGTCGGACGGCTTAAAGTACCCAAA‑GGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGc < 1:295860/241‑1 (MQ=255) ccgacgagccggcataaaacaCCCAA‑TGCCCGCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTg < 2:165410/231‑1 (MQ=255) gACGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTg > 1:165410/1‑244 (MQ=255) gcgcaatgaagcaacccatcgcc‑gccCCCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGGCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTGCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAAt < 2:14497/231‑1 (MQ=255) gCATTAAGTACCCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAg < 1:81362/251‑1 (MQ=255) gtacccaactccc‑gcccgcCTGTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTGCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTAt < 2:13265/240‑1 (MQ=255) ccgccgccTTTAGCAGATCGTCAAGTTCCAAGCTCGCGGGCAGTCGAACCTGCAGGCGCAATTCTTCGTGACGCCCCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAACCAGCCATGCGTTATATGAGTTAACGGCATGATTATCCGTTGCTTCAGGGTTTTTCATCGGCCGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACa < 1:592/248‑1 (MQ=255) | CTACCTCAGTTTCCGTGGCGTTGCGGCCAAGTTCCTGCTCCAGTTGCCCTATTGCCTGTGCCACTTCACGCGCGTTGCGTCGCACGCTGCGCGGCACCCAGTCACGGCTGCGAAGTTC‑ATCCAGCATAGCGCCACGGATACGCTGCACTGCGTAAGTTGTAAATGCCGTTCCTTGT‑AGGGCGTCATAGCGTTCGACGGCATTAAGTAACCCAA‑TGCC‑GCCCGCCTGTAGCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACA > NC_000913/2001429‑2001895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |