Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,000,613	C→A	E138* (GAA→TAA)	fliZ ←	DNA‑binding transcriptional regulator FliZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,000,613	0	C	A	100.0%	48.3 / NA	14	E138* (GAA→TAA)	fliZ	DNA‑binding transcriptional regulator FliZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (4/10);  total (4/10)

ACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTCATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTC  >  NC_000913/2000390‑2000853                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 cccccGAATGTTGTTATGTCGGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATc                                                                                                                                                                                                                       <  2:117299/250‑1 (MQ=255)                      gTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCgt                                                                                                                                                                                                   <  1:32471/250‑1 (MQ=255)                                                aaaCACAAAAACAACTCCGCTACATCTGATTCTTATTGAATAGATATCAGAAGAAGGCAGGCTGGAGGAGTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCGGGGGGTGGGCAGCCACGGTGCGAGGATTGAGGCATGGAAACCGTCCTGCAGCGGATCGAGTGAATTTTTTTGCTTGTGGAGACGGTTCTCCAAACGGCGCAaac                                                                                                                                                                        <  2:316140/251‑1 (MQ=255)                                                                     aCATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCg                                                                                                                                                   <  1:239337/251‑1 (MQ=255)                                                                                         atatatCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGt                                                                                                                                <  1:168443/250‑1 (MQ=255)                                                                                           atatCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTg                                                                                                                             >  1:157773/1‑251 (MQ=255)                                                                                               cAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTc                                                                                                                          <  1:19817/250‑1 (MQ=255)                                                                                                agaagaAGGCAGGCTGGAGGATTTCTGCACAAGCATGGAACAGGTTTGGCGCTGATAGTGTTGATAATTCCGTAACGCGATGCGGAAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTAATCAAGGACACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCACCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTcc                                                                                                                         >  1:294406/1‑249 (MQ=255)                                                                                                                          gCACAAGTCTGGTACAGGTTTGTCGGTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACTGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCTCCGTACCTGGGCTCATTTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGataa                                                                                                <  1:202309/249‑1 (MQ=255)                                                                                                                                      gacatggttGGCGCGGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTTGTGGGCAGCCACGGGGCGAGGATTTAAGCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGGGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATGTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGATAATATCGAAAAAATCa                                                                                  <  2:170595/244‑1 (MQ=255)                                                                                                                                                                                                             aGCCACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTgcc            >  1:30716/1‑250 (MQ=255)                                                                                                                                                                                                                cACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGcac                                           <  1:159951/216‑1 (MQ=255)                                                                                                                                                                                                                cACGGGGCGAGGATTTAATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGcac                                           >  2:159951/1‑216 (MQ=255)                                                                                                                                                                                                                      gCGAGGATTTAATCAAGGAAACCGGCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTtc  <  2:157773/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCGATGCGGTAATTGTTGGTGCTGGTGGTGGGCAGCCACGGGGCGAGGATTTCATCAAGGAAACCGTCCTGCAGCTGATCGAGGGAAATATTTTGCTCGTGTAGATGATTCCCCAAACGGCGCAAACGAACGACATATTCACGCACCGTACCTGGGCTCATTTCAGTTTGCTCAAAAAGAAATTGCTTAAAGCCGATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTC  >  NC_000913/2000390‑2000853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑