| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,964,698 | A→T | L118H (CTC→CAC) | flhA ← | flagellar biosynthesis protein FlhA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,964,698 | 0 | A | T | 89.3% | 72.7 / NA | 28 | L118H (CTC→CAC) | flhA | flagellar biosynthesis protein FlhA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (14/11); minor base G (0/3); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
CACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAGGTCGGCGTCAATCGCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGAGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTAAACAACAGGTCGAGTATGAATGCGGGCAGTGGCAGC > NC_000913/1964487‑1964943 | cACTTCGGAGCTGCGTGTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAGGTCGGCGTCAATCGCCATCTGCGTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGGTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCGTCGACCACCTTCCCTgccgcc < 2:308326/250‑1 (MQ=255) gcGGAGCTGCGTTTTTTCGCCTCACCTTCACCAATCAATCCGGCGTTAAGGTCGGCGTCAATCGCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGGGGAAGTGACCGAACGCTTCGACCACCTTCCCTgccgccgcc < 2:114452/248‑1 (MQ=255) gggcgGTTTGTCGCCTCATCTTCACCAATCAATCCGGCGTTAAGGGCGGCGTCAATCTCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCTACTTCTGCGATACGCCCGGCCCCTTTGGTAATGACCATAAAGTTGATGATCACGAGACTGACAAACACCACGATACCGATAGCGAAATTGCCTCCAACGGGGAAGTGATCGATCGCGTCGACCACCTTCCCTGCCGCCGCTGCGCCGGt < 2:311037/250‑1 (MQ=255) cATCTTCACCAATCAATCCGGCGTTAAGGTCGGCGTCAATCGCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGTTATGCCCTTCCATTa < 1:76713/249‑1 (MQ=255) tCACCAATCAATCCGGCGTTAAGGTCGGCGTCAATCGCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGATTCGACCACCTTCCCTGCCGCCGCCGCGACGGTATGCCCTTCCATTAAAATGAt > 1:66071/1‑251 (MQ=255) gTTAAGGTCGGCGTCAATCGCCATATGCTTACCCGGCCAACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACATTTGGAAATGACCATAAAGTTGATGATCACGAGAATGACAAACAACAAGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACATTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTGAAATGATACGGGTTGAAGCCACGt > 2:296532/1‑251 (MQ=255) cGGCGTCAATCGCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGcc > 1:176762/1‑251 (MQ=255) ggCGTCAATCGCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACAGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGcc > 2:80559/1‑250 (MQ=255) cccGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGacc > 2:48596/1‑156 (MQ=255) cccGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGacc < 1:48596/156‑1 (MQ=255) cgcgcACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGAATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACTACAGAATGGTCGGAAACGCAGCaa > 2:217711/1‑250 (MQ=255) cTGCGAAAAGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGCAGTAAACGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGGTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAACCTCAAGCGTgcac > 2:301324/1‑249 (MQ=255) cccGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAAc > 1:136400/1‑250 (MQ=255) aTGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGCAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAaca > 2:11206/1‑250 (MQ=255) gTTGATGATCACGAGAATGACAAACACCACGATACCTATAGCGAAATTGCCACCACCGTGGAAGTGACCGAACGCTTCTTCCCCCTGCCCTGCCGACGCCGCGCCGGTATGCCCGGCCATAAAAATTATACGGGGTGAAGCCTCGTTAAGTGCCAGACGCAACAGCGTTGTAAACAACAGAATGGTCTGATACGCAGCGAACTCAAGCGTGCGCTTTGGAAACAGCGCCATCAGCACCGCCATTATCGACa < 2:29183/251‑1 (MQ=255) gTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCTTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACa < 1:11206/251‑1 (MQ=255) gatCACGAGAATGACAAACACCACGTTACCGCTCGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGAATGCCCATCCATTAAAATGATACGGATTGAAGCCACGTTAAGTACCAGACGCAACAGCGTGTTAACCAACAGAATTGTCGGAAACACAGCAAACTCAAGAGTGCGCTGGGTAAACAGCCACACCATAAACACCAAGATCGAAAAGGCa > 2:169958/1‑250 (MQ=255) gatCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCaa < 1:80559/251‑1 (MQ=255) atCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAat > 1:174677/1‑251 (MQ=255) agaATGACAAACACCACGTGACCGATATCTAAATTGCCACCATCGGGTAAGTGGCCGAACGCGTCTACCACCTGCCCTGCCGCCGCCGCTCTGTGATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGTCGCAACAGCGGGGTAAACAACAGAATGGTCGGAAACGCAGCAAGCTCAATCGTGCGCTGGGTAAACAGCGCCACCAGCAACACCATGATCTGCAAGGCAATATTg < 2:285914/249‑1 (MQ=255) gACAAACACCACGATACCGATAGCGAAATTGCCTCCAACGTGGAAGTGACCGAACGCTCCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTTCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTaa < 1:163265/251‑1 (MQ=255) gACAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTa > 1:125475/1‑250 (MQ=255) aCAAACACCACGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTaa > 1:43451/1‑250 (MQ=255) cAAACCCCACGATCCCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGAATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGACTGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGAGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTCaac > 2:156933/1‑251 (MQ=255) cAAACACCACGAGACCGATAGCGAAATTCCCACTAACGTGAAAGTGACCGACCGCTTCGACCACCGTCCCGGCCGCCGCAGCGCCGGTATGCCCTGCCATGAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGGAGTGTCGGAAACGCAGCAAACTCAAGCGTGCGCTTGGTAAACATCACCACCAGCAACACCGTGATCGACAAGACAATATTGATGGTAaac < 2:261863/251‑1 (MQ=255) cccacGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTAAACAACAg < 1:211698/250‑1 (MQ=255) acGATACCGATAGCGAAATTGCCACCAACGTGGAAGTGACCGAACTCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATGCGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAGCACCATGATCGACAAGGCAATATTGAAGGTAAACAACAGGTc < 1:296532/251‑1 (MQ=255) caACGTGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTAAACAACAGGTCGAGTATGAATGCGGGCAGTGgcagc < 1:217711/251‑1 (MQ=255) | CACTTCGGAGCGGCGTTTTTTCGCCTCATCTTCACCAATCAATCCGGCGTTAAGGTCGGCGTCAATCGCCATCTGCTTACCCGGCATACCATCGAGAACAAAGCGCGCACCCACTTCTGCGATACGCCCGGCACCTTTGGTAATGACCATAAAGTTGATGATCACGAGAATGACAAACACCACGATACCGATAGCGAAATTGCCACCAACGAGGAAGTGACCGAACGCTTCGACCACCTTCCCTGCCGCCGCCGCGCCGGTATGCCCTTCCATTAAAATGATACGGGTTGAAGCCACGTTAAGTGCCAGACGCAACAGCGTGGTAAACAACAGAATGGTCGGAAACGCAGCAAACTCAAGCGTGCGCTGGGTAAACATCGCCACCAGCAACACCATGATCGACAAGGCAATATTGAAGGTAAACAACAGGTCGAGTATGAATGCGGGCAGTGGCAGC > NC_000913/1964487‑1964943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |