Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,876,382	T→A	I237N (ATC→AAC)	nimT →	2‑nitroimidazole exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,382	0	T	A	100.0%	39.4 / NA	14	I237N (ATC→AAC)	nimT	2‑nitroimidazole exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/8);  total (6/8)

CGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCT‑GATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTG  >  NC_000913/1876145‑1876626                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      cGCTGGCTTTGAAAGGTTTTGGCTGGCAAGGCGCGTTGCTCCTGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGAGAAGTCAACAACCTGCAAATTTGAGTACCTAGCGCGCCTTACATACTAGGGGTATCTGGAGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTATGTGATTATTGGCTGGCTTCCGGCGAACCTCCTCAGTCAc                                                                                                                                                                                                                                          >  2:153703/1‑251 (MQ=255)                                               gATGTGTTTTCCTCTGCTGTCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAAc                                                                                                                                                                                            <  2:58730/251‑1 (MQ=255)                                                aTGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACt                                                                                                                                                                                           <  1:237834/251‑1 (MQ=255)                                                                       ttttttATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTg                                                                                                                                                                     <  1:117556/250‑1 (MQ=255)                                                                                              gAAGTCAACAACATGCAAAGTTGAGTACCTCGCGCGCCGTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCT‑GATCCCACTTTTCTTAcatcat                                                                                                                                            <  1:25007/251‑1 (MQ=255)                                                                                                    aacaacATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCT‑GATCCCACTTTTCTTACATCATGTGaaa                                                                                                                                      <  1:189077/251‑1 (MQ=255)                                                                                                                  ttGAGTACCTCGCTGGGCTTACATACTCGGGGTAGCTGTCCTTCAACGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATGACGTGATTATTGGCTGGCTTCCGGCGAACCGCATTAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTGGGCTGCAACTAGCCACAGCAGCACCCGGTTTGCT‑GATCCCACTTTTTTTACATCATGTGAATGAGCAGCGTGGTa                                                                                                                         <  2:83665/250‑1 (MQ=255)                                                                                                                     aGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTAACTGGTCTATTACGTGATTATTGGCTGGCTTCAGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCTCTGCCAGGGTTGCTGCAACTAACCACAGCAGGACCCGGGGTGCA‑GATCCCCCATTTTTTTCAACACGTGAAAGaaaagagtgggattg                                                                                                                      >  1:175349/1‑236 (MQ=255)                                                                                                                                                             cACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGCTCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTTCTGCAACCAACCACCACAGCACCCGGTTTGCTTGTTCCCCCTTTT‑TTTCATCCCTTGAAAAAACAGCGTGGGATTGCAGCGTTCGTTTCCCTTATGTGCGCAGGCGGGGCGGTTggg                                                                             >  2:133716/1‑251 (MQ=255)                                                                                                                                                                                                      tCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGGTCCCTTCCTGGTTTGCTGAAACTAACCAAAGCAGCAACCGGGTTGGT‑GGTCCCACTTTTTTTTCATCCTGTGGAAGATCAGAGTGGGATTGCGGGGTTCGTTTCCTTTATGTGGGACGTGGGGGGGGGTGGGGTCTGGTTTTTGCCAGCGCACGCGGTGACCTGGACTCTggc                                    >  2:118240/1‑249 (MQ=255)                                                                                                                                                                                                                          tattGGCTGGCTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCT‑GATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTGGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCTCCTGGACTCTGCTTTTCGGTTTTGGTTCCGgc                 <  1:309544/250‑1 (MQ=255)                                                                                                                                                                                                                             tggctggcTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCCCTGCATGGGTTGCTGCAACTAGCCACAGCCGCACCCGGTTTGCT‑GATCCCACTTTTCTTACATCATGTGa                                                                                                                                        >  1:112948/1‑128 (MQ=255)                                                                                                                                                                                                                             tggctggcTTCCGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCT‑GATCCCACTTTTCTTACATCATGTGa                                                                                                                                        <  2:112948/128‑1 (MQ=255)                                                                                                                                                                                                                                        cGGCGAACCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGGTCACTGCATAGGTTGCTGAAACTAAACACCGGAGCACCCGGTTTGCT‑GATCCCACTTTTTTTTCATCATGGGGCAGAATAGAGTGGTATTTCAGCGTTCGTTTCCTTGATGTGCGCAATGGGGGCGGTTTGGGTCTGCCTTTTGCCAGCGGACCGGACTACCCGGGCTCTGGTTTTTGGGTTTTGTTTCGGCGCAACAATGATAcgg  >  1:299775/1‑249 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCT‑GATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTG  >  NC_000913/1876145‑1876626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑