Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	100.0%	50.3 / NA	20	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

CCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGG  >  NC_000913/1829661‑1830112                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  cccGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCAGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTATCCTCTGCGCACAGGAGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGATAAAATCAAACGAACGGATGCCGGATCGGTACGCCACAGC‑ACCACGCGGTAC‑CGGGGATAATTTTCATTAGCGGCCAGGCAGGCTGGGAAATCg                                                                                                                                                                                                           >  1:255753/1‑251 (MQ=255)        ccGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGGTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTgcg                                                                                                                                                                                                    >  1:309193/1‑251 (MQ=255)            aTTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCATTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGTCATTTGAGGGCATCCCGTTGTGCGGCGGTTAAAATCAAACGCCCGGTAGCCGGCTCGGTACGCACCAGC‑CCCACCCGGTAA‑TGGTGAGAATTTTCCATAACGACCAGGCAAGCTGGGCGGTCGCCCTGCGCAgg                                                                                                                                                                                                >  2:171532/1‑251 (MQ=255)                                             tATCCAGTCACCGTTAATCCATAAAGTCATGCTGTGTTCTCCTCTGCGCACAGGCGCACCAGACTAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGTCGTTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCGCCAGC‑TCCACGCGGAAA‑TGTTGATAATTTTCATTATCGACCAGTCAGGCTGGGAAATCGCCCTACGCAGGCGGCCCTTCGTCCACTTCCGCCAGCCGACttt                                                                                                                                                                <  2:294246/250‑1 (MQ=255)                                              aTCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGTAAATCGCCCTGCGCAGTCTGCCCTTCTGCCACTTCCACCAGCCGACtttt                                                                                                                                                               <  1:128543/250‑1 (MQ=255)                                                   gTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCGTCTGCCACTTCCACCAGCCGACTTTTACGGAt                                                                                                                                                         <  1:97703/251‑1 (MQ=255)                                                            aaTCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAc                                                                                                                                                <  1:89151/251‑1 (MQ=255)                                                                  tAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGGAA‑TGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCgggc                                                                                                                                          >  1:7714/1‑249 (MQ=255)                                                                                               caGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCgc                                                                                                             >  1:106407/1‑250 (MQ=255)                                                                                                aGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGAt                                                                                                                                                         <  1:206697/206‑1 (MQ=255)                                                                                                      cccAGACGAACGCGAGCCCCGGCGTGGCATTTGAGGGCAGCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAt                                                                                                      <  2:106407/250‑1 (MQ=255)                                                                                                              aaCGCGCTCCCCGGTGTGGCTTTTGATTCCTTCCAGTTTTGCGGCGGTTAATATCTACCGCTTGCTTTCCGGTTCGGTACTCTCCAGC‑ACCCCGCGGAAA‑TTGTTATAATGTTCATTATCCAGCCGGCGATCTTGCAGATCGCCCTGCGGAGGCCGCTCTTCTGCTGCTTCCGCCAGCCGACTTTTGCCGATGGCTCTTACGCGTTCAATGTCACACTCAAGCGTCGGCCCACCGTCAACGATCTCTatat                                                                                              <  2:107641/251‑1 (MQ=255)                                                                                                                       cccGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGATAAAATCAAACGCTCGGATGCCGGGTCGGGACGCACCAGCCACCA‑GCGGAAA‑TGGTGATATTTTTCATTAGCGACCAGGCAAGCTGGGAAATAGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCACCCGACTTTTAAGGAAGGCGCGCCCGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAATCTGTCGATATAGTTAccgt                                                                                     >  1:193587/1‑248 (MQ=255)                                                                                                                        ccGGCGTGGCATTTGAGGGCATCCAGTTGTACGGCGGCAAACATGAAACGCTAGGTTGCCGGATCGGTACGCACCAGC‑CCCCAGCGGAAC‑TGGTGATAATTTTCATTAGCGACCAGGCCGACTGGGAAATCGACCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGCCTTTTTCGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCACAGATGTCGATATTGTTACGGt                                                                                     >  1:302781/1‑250 (MQ=255)                                                                                                                                gcagttGAGGGCCTCCAGTTGTGCGGCGGTTAAAATCAACCGCTCGGTTGACGGATCGGGACGCGCCAGC‑ACCGCGCGGAAA‑TGGGGATAATGTTCATTAGCGCCCAGGCAGGCTGGGAAATCGCCCTGCGCTGGCTTCCCTTCTGCCACTTCCACCAGCCGACTGTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAAc                                                                            <  2:3348/247‑1 (MQ=255)                                                                                                                                                  ttGTGCAGCGGTTAAACTCAAACGCTCGGGTGCCGGATCGGTACCGACCAGC‑ATCAAGCGGAAAGTGGT‑ATAATATTCATTAGCGACCAGGCAGGTTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTGTCGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCTAAGATGGCGATATAGTTACTGTAGCGAAAACCTTCTTTCTCCAGCAcc                                                           <  2:175605/250‑1 (MQ=255)                                                                                                                                                                    cAAACGCCCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAATGCTTCTTTCTCCAGCACCGCGCGTGCAGGCGCGGtt                                         <  2:309193/250‑1 (MQ=255)                                                                                                                                                                                            gactcaccagtcccacTCGGAAA‑GGGTGATAATGTTCATGAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACGTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGGCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTGTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGTATTTACCTGACCGATGACg                 <  2:255753/239‑1 (MQ=255)                                                                                                                                                                                                   agc‑accaCGCGGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCGGGAAAATAGCACTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCTGCCCAACGTCAAAGATGTCGCTATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGAGGATGTAACTTACCGATGACGTCCTggac         >  1:126031/1‑249 (MQ=255)                                                                                                                                                                                                           cgcgGAAA‑TGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGCCTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGCTATAGTTAAGGTAGCGAAAACCTTCTTTCTCCAGCACCCCGCGGGCAGGCGCGGTTTTCGGATGCACCTGACCGCTGACCTCCTGGGCGTCCTgg  >  2:168095/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  CCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGC‑ACCACGCGGAAA‑TGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGG  >  NC_000913/1829661‑1830112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑