| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 185,096 | A→G | intergenic (‑27/+27) | yaeI ← / ← dapD | phosphodiesterase YaeI/tetrahydrodipicolinate succinylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 185,096 | 0 | A | G | 97.1% | 99.1 / ‑6.1 | 35 | intergenic (‑27/+27) | yaeI/dapD | phosphodiesterase YaeI/tetrahydrodipicolinate succinylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (19/15); minor base C (1/0); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACG‑CGACCGTAGTGGATTTCGCCGGTTTCACGGTCGTAAATACGGGTGCTCTGACCAATGTATACGCCC > NC_000913/184865‑185336 | aaaTCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGGTTAATTCAAAACAGCCTGGCTCACAGTAATGCCTATAACCGCAAACTGCGCTCGGGGCTATCGTCGCGGCCGTTACCTGCAAAAAATGGCGGCGGGGAATCATCGACCATCCCTGCCAACAAAACGGGAGGCGGCGGGCTGCCCGcgtg > 2:124599/1‑251 (MQ=255)aaaTCAGGCTTAAAGGAACAAAAAGCGAGTAATGGCGCTCGGACAGAAAACGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGAAGGGTTAATTCAAACCAGCCCGGCTTACAGAAATGCATATTACCGAAACCTGAGCTCATGGCTATCGGCTCGGAAGAAGCATGCAAAAAACGGCGGCGTGGAATCATCGCTCATTCCTGCAAAAAAATAAGGCGGCCTCGTGGTGCCCgcg > 2:207285/1‑247 (MQ=255) aaTCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCGGCGTTGTCTTTAAAAAAGGCGATGCGGGGACTTATTAATTCAACTCAGCCGGGCTCACAGGAATGCATATAACCGAAACAGGATCTTGGGGTTATTGTCGCGGCAGTAGCGTGCAAAAAGCGGCGGGGTGAAATCATCGTTCATCCCTGCAAAAAAATCGGGGGGCGTCGTGCTGCCCgtgttc < 2:310097/251‑3 (MQ=255) ggCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACt < 2:133672/251‑1 (MQ=255) aaaaCGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTTCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAAGCGGCGGCGTGAAATCATCGCTCATCCCGGCAAAAAAATCGTGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGt < 1:256657/251‑1 (MQ=255) aCGAGAGCAATGGAGATCGGCCAGAAAAAGACTTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGAAGGATTAATTCAAACCCGCCTGGCTCAACGCAATGCATATAACCGAAACCTGAGCTCGTGGGTAACGTCGCGGCAGGTGCCTTCAAAAATCGGCGGCGTGAAATCCTCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTTCCCGTGTGCGTACCTTTCTTCGCTGGTACg > 1:28388/1‑251 (MQ=255) gaTCGGCCTGAAAACGAATTTTGAAAGGTGCTGCGTTGTCTTAAAAAAAGGCGAGGCGGTGACGTATGAATTCAAACCAGCCGGGCTCACAGTAATGCAAATAACCGAAACCTGCGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCGGCCCGTGGGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTag < 2:174425/251‑2 (MQ=255) aTCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAAt < 1:5171/251‑1 (MQ=255) cGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGc > 1:11191/1‑251 (MQ=255) tttGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAg < 1:56199/250‑1 (MQ=255) tGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACgcagc < 1:227101/220‑1 (MQ=255) aTGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAAACAACCTGGGTCAAAGTAAAGCATATAAAAGAAACCTGAGCTCGTGGCTCTCTTCGCGGCAGTAGCCTGCAAAAAACGGCGGCCAGAAATCATCGCTCATCCCTGCAAAAAAAACGGGCCGCGTCGTGCTGCCCGTGTGCATACTTTTTGTACATGGTGCGCACCAGTTCGTTAAAGCCCACTTTTCCGCGAGGTTTTc > 1:309190/1‑251 (MQ=255) ggTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGGTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCCGCAAAAAAATCGGGCGGCGGCGTGCTGCCCGTGTGCATACTTTTAGTCGATTGGAAGCAGCAGTTCGTTAATTCCGACTTTGCCGCGGG‑TTTTcgcg > 1:10356/1‑251 (MQ=255) gcgGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATAGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGt > 1:301508/1‑250 (MQ=255) cgGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTag < 1:16813/251‑1 (MQ=255) tAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTAttt > 1:53294/1‑251 (MQ=255) cAGCCTGGCTCACAGTAATGCCTCTAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGACTGCAACAATCGGCGGCGTGACATCATCGATCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATAATTTTAGTCGATGGTACGCAGCAGTTCGTTACTGCCGCCTTTGTCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATCACAGCCCAGTAGCGGCTGTCTTTGCCAtttttt > 2:117979/1‑251 (MQ=255) ggCTCATAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGc > 1:89185/1‑251 (MQ=255) ggCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACg < 2:11191/249‑1 (MQ=255) acaGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGAtt > 1:142443/1‑251 (MQ=255) caGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTGCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTa < 1:122377/251‑1 (MQ=255) ggCATATAAACTAATCCTGAGCTCGTGGATTTCTGCGCTGAAGTAGACTGCAAAATTCGGCGGAGTGAAATCATAGATCATCCCTGCAAAAAAATCGGGCGGCTTCGTGCTGCCCGTGTGCATTCTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAATTTTCTTAACGATAAACGCACAGTAGATGCTGTATTTGCCATCTTTTGACGGCAGATTCCCTGac < 2:74070/250‑2 (MQ=255) gcctataACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGaa < 2:74059/247‑1 (MQ=255) cATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAaac > 1:157118/1‑251 (MQ=255) aaCCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACca < 2:53294/251‑1 (MQ=255) ccGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACcac > 1:76104/1‑250 (MQ=255) gAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAAGCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTGTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATTTTTTGACTGCAGATTACCTGAAACAACCACAGACCccg < 1:30015/249‑1 (MQ=255) gctcgagcccctcgtcgCGGCCGTAGCCTGCCAAAGTCGGCGGCGTAAAATAAACGCCAATCCCTGACACAAACTCGGACGGCGTCGTGGTCCCCGTGAGCATACTTTTAGTCAATGGTACGCCGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCACCTTTCTTAACGCTAACCGCACAGTAGAGGCTGCATTTGCCATCTTTTGACGGCAGATTACCTGAAACCACCAAAGACCCCGCCgg > 2:284571/12‑251 (MQ=255) ggCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAACAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAAcg‑cg > 1:102976/1‑251 (MQ=255) aaaTCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTAGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGCATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGATCGACCAC‑GTAGTGGCTTTCGCCGGttgcccg > 2:1651/1‑246 (MQ=255) ggcgTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACG‑CGACCGTAGTGGATTTCGCCGGTTTCACGGTCGTaa > 1:99638/1‑250 (MQ=255) catcGCTCATCCCTGCAAAAAAATTGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACG‑CGACCGTAGTGGATTTCGCCGGTTTCACGGTCGTAAATACGGGTGc < 1:180032/250‑1 (MQ=255) aaaaaaTCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACG‑CGACCGTAGTGGATTTCGCCGGTTTCACGGTCGTAAATACGGGTGCTCTGACCAATGTATACGc > 2:169939/1‑251 (MQ=255) aaaaaaTCGGGCGGCGTCGTGCTGCCCGTGAGCATAATTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGAATTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTCTTTGCCATCTTTTGAAGGCAGATTACCTGCAACAACCACAGACCCCGCCGGCACG‑CGACAGTAGTGGATTTCGCCGGTTTCACGGTCGTAACTACGGGTGCTCTGACCAAGGTATACGc > 2:25616/1‑251 (MQ=255) aaaTCGGGCGGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACG‑CGACCGTAGTGGATTTCGCCGGTTTCACGGTCGTAAATACGGGTGCTCTGACCAATGTATACGccc > 1:10251/1‑250 (MQ=255) | AAATCAGGCTTAAAGGAACAAAACGAGAGTAATGGAGATCGGCCAGAAAAAGAATTTTGAATGGTGCTGCGTTGTCTTTAAAAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAG‑TTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTATTTGCCATCTTTTGACGGCAGATTACCTGAAACAACCACAGACCCCGCCGGAACG‑CGACCGTAGTGGATTTCGCCGGTTTCACGGTCGTAAATACGGGTGCTCTGACCAATGTATACGCCC > NC_000913/184865‑185336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |