Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,726,152	T→C	S44P (TCC→CCC)	nemR →	DNA‑binding transcriptional repressor NemR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,726,152	0	T	C	100.0%	34.0 / NA	14	S44P (TCC→CCC)	nemR	DNA‑binding transcriptional repressor NemR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/5);  total (9/5)

TCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCG  >  NC_000913/1725913‑1726395                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    cccGCCACTTCTCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTACTGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGCACATCTCCTGGCGACGGGCTATCAACTTTGCCTGCAACTTGGATTCACCGGGATGGCTCTAAGCGACTTACTAAAAACCGCTGAAGTGCCTAAAGCGCCCTTCTATCac                                                                                                                                                                                                                                          <  2:59074/250‑1 (MQ=255)                   tcAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAaaga                                                                                                                                                                                                                         >  1:48545/1‑250 (MQ=255)                                   ccATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCa                                                                                                                                                                                                        <  2:108392/251‑1 (MQ=255)                                                          cAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTAcgccgc                                                                                                                                                                                  >  1:17456/1‑250 (MQ=255)                                                                                                                 gAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGAcc                                                                                                                          <  2:115487/251‑1 (MQ=255)                                                                                                                         acacCGAACATGATACTCGCGAACATCTCCTGGCGACGTGCGAGCAACTTTGCCTGCAACGTGGATTCACCTGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAg                                                                                                                                                                        <  1:300519/197‑1 (MQ=255)                                                                                                                         acacCGAACATGATACTCGCGAACATCTCCTGGCGAAGGGCGCGAAACTTTGCATGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGCAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAg                                                                                                                                                                        >  2:300519/1‑197 (MQ=255)                                                                                                                                             gAACATATCCTGGCGGCGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTCCAGCGACGGCATACTGGATTATTACCAGCAAacac                                                                                               >  2:77843/1‑250 (MQ=255)                                                                                                                                                        ggCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCCAACACTGAACCAGtttt                                                                                   >  1:292702/1‑251 (MQ=255)                                                                                                                                                                  gAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATgg                                                                         >  1:832/1‑251 (MQ=255)                                                                                                                                                                                                      gggCTAGGCGAATTACTAAAAACCGCTGAAGTGCCGACAGGGCCCTCCTATCACAACTCTCGTTCTAAAGAAGCGTTTGGCGCTGCCATGCTTGAGCGCCATTACGCCGCATATCACCAGCGACTGACTGCGTTGCTCCAATCCGGCGACGGTAACTACCGCCACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACACGGACCCATCAGTGGTTGACTGACAGTAAAACTCTCTGc                                     >  2:101149/1‑251 (MQ=255)                                                                                                                                                                                                                    aCTAAATACCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATc                       >  1:87600/1‑251 (MQ=255)                                                                                                                                                                                                                         aaaCCGCTGAAGTGCCGAAAGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTTCCTCGACCGCTTACTGGCTTATTACCAGCAAACACTGAACCAGGTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCa                  <  1:147519/251‑1 (MQ=255)                                                                                                                                                                                                                                         ggaaGGGCCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGAGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTAGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTACCGCAGCGTGCGATCTGTCAGAAGATATGCGCAgcg  >  2:60610/3‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    TCCGCCACTTCGCCCTCCTCAGATAAGATTATTACCATTATTGAAGCTGTTAATGTCCAAAGTAGCAACTTTGCTTGCACTAGACCGACTGGTCTACTACACTCCAACGCATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCG  >  NC_000913/1725913‑1726395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑