Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,954	G→A	S168S (AGC→AGT)	ynfC ←	PF06788 family lipoprotein YnfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,954	0	G	A	100.0%	45.8 / NA	16	S168S (AGC→AGT)	ynfC	PF06788 family lipoprotein YnfC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

CCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCA  >  NC_000913/1656709‑1657189                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            ccGGGTCAGACGAGCCTGAAGCAGACCGAACAATAGCATGAAGAAGAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCATCGGATTAGCGTGACTGTCATATTCACAGCGCTGTTTTACATTACCAACCCGTTGATTAGTCAGTAAAGTAACCGCGGTGTAATCTAATTTTTTGATCGTATCCGTTGATGGCGTGGCACTGAc                                                                                                                                                                                                                                        <  2:114690/251‑1 (MQ=255) ccGGGACAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAA‑AATGGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCAACAAAAATGATTAACTGACAGTCGACCGGCTTAGCGTGACTGTCATATTCACAGCTCTGTTTTAAATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGAACGGATCCGTTGATGGCGTGGCACTgcc                                                                                                                                                                                                                                        >  1:60808/1‑248 (MQ=255)                                                   gacacgaTTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCATCATCAACAATGATTAGCTGACCGTCCACCGGATTAGCGTGACTGACATATGCACAGCTCCGTTTTACATTACCATCCCGTTGATGATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGTATCCGTTTATGGCGTGGCACTTACAGATAATTTTGTGTCGTTACTTTTCGTGGTTTTACCCAgtgtacaaccat                                                                                                                                                                                      <  2:149373/245‑12 (MQ=255)                                                                                                        caacaaTGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCt                                                                                                                                                                                      >  1:314606/1‑198 (MQ=255)                                                                                                        caacaaTGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAAGGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCt                                                                                                                                                                                      <  2:314606/198‑1 (MQ=255)                                                                                                                aTTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCAtt                                                                                                                         >  1:124788/1‑251 (MQ=255)                                                                                                                                  ggATTAGCTTGACTGTCATATTCACAGCTCTGTTTTTCATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTCCCCAGCGGATAACCCTGATCATCATAGCGATATTGCATCGGCATTTGTTTGCTGCTGGCTTGAATCGCGAAGCCATTATCATCGGTTTCCCAACt                                                                                                       <  2:22954/251‑1 (MQ=255)                                                                                                                                                           aGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCCCCCCGGCAGAAGGTAATTCTGCt                                                                              <  2:114857/251‑1 (MQ=255)                                                                                                                                                                       cATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCAtttt                                                                  <  1:222534/251‑1 (MQ=255)                                                                                                                                                                          tACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGAAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTccc                                                               >  1:111573/1‑251 (MQ=255)                                                                                                                                                                                   gTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGCTCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGt                                                      >  1:297977/1‑251 (MQ=255)                                                                                                                                                                                      gATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTAct                                                   >  2:262512/1‑251 (MQ=255)                                                                                                                                                                                      gATTATTCAGTAAAGAAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTAct                                                   >  1:246909/1‑251 (MQ=255)                                                                                                                                                                                                                          ttttGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGtaata                   >  1:735/1‑247 (MQ=255)                                                                                                                                                                                                                          ttttGATCGGATCCGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGtaata                   <  2:735/247‑1 (MQ=255)                                                                                                                                                                                                                                       cGTTGATGGCGTGGCACTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCa  <  2:246909/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gtggcaCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCt                                                              <  1:247967/181‑1 (MQ=255)                                                                                                                                                                                                                                                 gtggcaCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCt                                                              >  2:247967/1‑181 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            CCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTACATTACCAACCCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCA  >  NC_000913/1656709‑1657189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑