Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	43.2 / NA	16	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/5);  total (11/5)

CTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGC  >  NC_000913/1652107‑1652557                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    cTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCTTCTTATTAAGCCCGCAATCGACGCCCTCCGGAGGCAGATGACCTTAAAGAGCCTGAGCAAAACGCATATCATTGTTGTTCACCCCAGAGAGGTTGGCAGAACAGaaaa                                                                                                                                                                                                            >  2:258150/1‑250 (MQ=255)  tGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAACGGGGCATTCCTCTTATTAAACCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAAACTGAGCAAAGAGGATATCATTGATGTTCACCTCCGCGAGGATTGCAGAACAGAAAAAc                                                                                                                                                                                                          >  1:163424/1‑251 (MQ=255)                                tGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGaa                                                                                                                                                                             <  1:258150/250‑1 (MQ=255)                                        aggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGGGTCAGATGAAATTAACGAGACTGCGCAAAGAGCATATCATTGATGTTCACCTAAGAGAGGATGGCAAAACAGAAAAAAAAAAATGCACCTTTTTTTTTTAACCTGAA‑GGGTCAGCg                                                                                                                                                                   >  1:44204/1‑251 (MQ=255)                                                            ggAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAACAGGGACCTTCCTATTATTAAAACCGCAATTGAAGCCCTCCAGAGTCAGATGACCTTAAAGAGACCGAGCAAAGAGCATATCATTTATGTTTACCTCAACGAGGATAGCAGAAAAGAAAAAAAAAAATGCACCTTTGGTTTTCAAACTGAAAGT‑ACAACGAGAGGAAAAAATTATGGTg                                                                                                                                                >  2:47476/1‑250 (MQ=255)                                                                 aaTGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTGCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCAtt                                                                                                                                          <  2:44204/251‑1 (MQ=255)                                                                                               ccGTTTCACGGTACCAAAAACACAGGCGGGAACCCATCGGGTCATTCATCTTATTTAACCAGCAATCGGCGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACCGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCAATTTACCGTTGACTCCATAAGGCAGATGTggg                                                                                                            >  1:189692/1‑251 (MQ=255)                                                                                                      aCGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTTATCTTATTAAGCCAGCAATCCACGCCCTCCGGAGTCAGAAGACATTAACGAGAATGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAAAACAGAAAAACAAAAAAGCACCTTTTTTTTTTAACCTGAA‑GTGTCAGCGAGAAGAAAAAATTATGGTGAACATTTTACCGTTGACTCAAAAAGGCAGATGTGGGAagcagc                                                                                                     >  2:311681/1‑251 (MQ=255)                                                                                                             cAAAACCACCGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGTGACGCAGCGGTaaaa                                                                                              <  2:28605/251‑1 (MQ=255)                                                                                                                        gCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCATTGTTTTTCAACCTGAA‑TTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGACGGACt                                                                                   >  2:208852/1‑251 (MQ=255)                                                                                                                                                               aTCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCATTGTTTTTCAACCTGAA‑TTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGc                                            <  1:208852/251‑1 (MQ=255)                                                                                                                                                                    cGCTCTCCGGAGGCAGATGACATTAACGAGACTGAGCAAAGTGCATATAATTAATGCTCACCTCAGAGAGGATGGAAGCACAGAAAAAAAAACATGAACCTTTGTTTTTCAACCTGCA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgct                                       >  1:138317/1‑251 (MQ=255)                                                                                                                                                                                      gACATTAACGAGACTGAGCAAAGCGAATATCATTGCTGTTCACCTCAGAGAGTCTGGCAGAACAGAAACACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTAGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTg                     >  2:160502/1‑251 (MQ=255)                                                                                                                                                                                                       gCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACCAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTAACGTTGACTCAATCAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat     >  1:216121/1‑250 (MQ=255)                                                                                                                                                                                                       gCAAAGAGCATATCATTGATGTTCACCGCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTtat     <  2:216121/250‑1 (MQ=255)                                                                                                                                                                                                         aaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGAAATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGc  >  2:94478/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    CTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAA‑GTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGC  >  NC_000913/1652107‑1652557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑