Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,525,172	C→T	G270D (GGC→GAC)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,172	0	C	T	95.2%	50.0 / ‑5.7	21	G270D (GGC→GAC)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/11);  minor base G (0/1);  total (9/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

GGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCAC  >  NC_000913/1524926‑1525400                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     ggCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGAc                                                                                                                                                                                                                                  <  1:122138/251‑1 (MQ=255)    acaTGCTAACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGAGCAGTCCAGACCTGAATTCAGACTGGAGAGGGCAGCGGTCAGCCCCACAATGTTCATAATGCTGCCGATATATGGCACACCAAGTTTAGAGAAAAACGTCACGAACGGACTTTACCCCGCCTGATACGCGCTCCACGGCACTAACATACACAGCACCACCACGGAGTCGACgg                                                                                                                                                                                                                                >  2:195619/1‑249 (MQ=255)        gCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACATAAAAc                                                                                                                                                                                                                           <  1:92462/251‑1 (MQ=255)                cTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACACCGGAGTCGACGTAAAACAGGCCAAt                                                                                                                                                                                                                   >  2:38186/1‑251 (MQ=255)                        cGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGAAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCaa                                                                                                                                                                                                            >  1:264127/1‑250 (MQ=255)                                                              aGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                                                                                                                                                                                      >  1:58179/1‑234 (MQ=255)                                                              aGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                                                                                                                                                                                      <  2:58179/234‑1 (MQ=255)                                                                     aaTACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCCCAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACCCCACGTAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTAc                                                                                                                                                              <  2:26125/251‑1 (MQ=255)                                                                         cGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATtc                                                                                                                                                          >  1:54533/1‑251 (MQ=255)                                                                                              gAATTCAGTCTGGAGAGGGAAGCGGTCAGCACCTCAATGTGCATAATGCTGCCGATATATGGCTCACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGAGACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGGAAAACAGGCCAATACTCTAAATCTCACTGTTAATGTGTTTAGGCTCCATGGTCTGCTGATCTTTACATTCTCCGGCAGCTGTACccacca                                                                                                                                      <  2:300731/250‑1 (MQ=255)                                                                                                   cAGGCTGGAGCGGGCAGCGGTCAGCCCCACAATGTTCATAATGCTGCCGATATATGGCACACACAGTTTAGAGCAACCAGTCACGAACGGCCTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGCGTCGACGTAAACCAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGACCGCGGATCTTTACATTCTACGGCAGCTGTACCCACCATTTCa                                                                                                                                 >  2:99636/1‑250 (MQ=255)                                                                                                         gtagagGGCAGCGGTCAGCACCACAATTTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTCTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGa                                                                                                                            <  2:226700/247‑1 (MQ=255)                                                                                                                     ggTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAac                                                                                                               >  1:198626/1‑250 (MQ=255)                                                                                                                                                   gATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCGCCACGGCAATAACATAACCAGCAACTCCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTGAATGGCTTTAGGCACCAGGGTGTGCGGAGCTTGACATTCTCCGTCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAGCACTACGCCCTGAATCAACTCCTGCgcaggca                                                                                <  2:106418/251‑1 (MQ=255)                                                                                                                                                    atatatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCgcaggcag                                                                               <  1:272992/251‑1 (MQ=255)                                                                                                                                                         gggCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAAc                                                                          <  2:77856/250‑1 (MQ=255)                                                                                                                                                            cacaCCCATTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTTATACGCGCTCCACGGCAATAACATAACCAGCAACGCCACTGAGTCGACGTAAAACAGGCCAATACGCCAAAGCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAAACAACACCAGCGCAGGCAGCAAACCGt                                                                       <  2:119679/251‑1 (MQ=255)                                                                                                                                                            cacaCCCAGTTTAGAGAAAAACGTCACGAACGGACTTGGCCCCGCCTGCTACGCGCCCCACGGCAATAACATAACCAGCAACCCCACGGAGGCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTTAATCAACACCAGCGCAGGCAGCAAACCGt                                                                       <  2:60815/251‑1 (MQ=255)                                                                                                                                                                                      cGAACGGACTTTGCCCCGCCTGATACGCGCTCCCCGGCAATAACATAACCACCAAAACCACGGAGTCGACGTAAAACAGGCCAATCCGCCAAATCCCACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAAACCCAGCGCAGGCAGCAAACCGTGGGGGCAGAAGCCGCCATTATCGGTg                                             >  1:130506/1‑251 (MQ=255)                                                                                                                                                                                               tttGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGTCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAACCCGTGGGGAAAGAAACCGCCATTATCGGTGATTAAATg                                     >  1:278396/1‑250 (MQ=255)                                                                                                                                                                                                                                 caaaacCAGAAACCCCACGGAGTCGACGTAAAACAGGCCATTACGCCAAATCACACTGTAAAGGGCTTTAGGCACCATGGTGTGCGGATCTTTCCATTCTCCGGCAGCTGTCCCCCCCAGTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTTGTGTTGCCATCCAGCGGCTGACCAc  <  2:198626/248‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     GGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAATGGAGGCAAAAGCAAACACTACGCCCTGAATCAACACCAGCGCAGGCAGCAAACCGTGGGGGAAGAAGCCGCCATTATCGGTGATTAAATGAAAGCCAGTGGTGTTGCCATCCAGCGGCTGACCAC  >  NC_000913/1524926‑1525400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑