| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,494,914 | G→T | T53N (ACT→AAT) | ydcI ← | DNA‑binding transcriptional dual regulator YdcI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,494,914 | 0 | G | T | 93.3% | 42.5 / ‑3.9 | 15 | coding (158/924 nt) | ydcI | DNA‑binding transcriptional dual regulator YdcI |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (5/9); minor base . (1/0); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAA > NC_000913/1494692‑1495158 | tgttgCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGATCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCt < 2:160416/250‑1 (MQ=255) aaGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAgg > 2:295609/1‑251 (MQ=255) ggtagttagccaggcctaacgacATCATTATTGAGACCTTCGTTACTATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCACCGGGGAAGGAAAGTTGCGCCCCCTGCCGACCACGCTCAAACAAGCGAGCGCCATTAA‑GCTGCTCCAGTTCCTTCATTGTCTTAGAGAGCGCAGGATGACTCCAAATAAGGGTTTCAGCCGCCCGCCCCACAGTTcc > 2:302497/19‑251 (MQ=255) gacATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGCTGGCGACAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGGAGGGAAAGTTGCACCCCCTGACGACAACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCACATTAAGGGTTTCAGCAGCGAGCACCACAGTTCCTTGTTGGGCGACAGCTACg > 1:93556/1‑250 (MQ=255) aaGACCTTCTTTACGATGAAGCGACCGTCCCGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAgg < 1:241932/250‑1 (MQ=255) ggACCATCTTTACGATGAAGGGACCGTACGGCAGGGATGATGGAGTCACGAACTCTGACTGCATGCGTCAACAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCACCCACGCGAGCGCCA‑TCAAGATGCTCCAGTTCATTCAATGACTTAGAGAGCGCAGCTTGCCTCCAATTAAGGGTTTAAGCCGCGCGCCCCACAGTTCCTTGTTCTGCGACAGCTACGATTGTATGCAgg > 2:179795/2‑249 (MQ=255) ccGGCAGTAGTGATGGCGGTAAGAACTCTGACTGCATTCGTTAAAAATTGTTCGCCGGGTAATGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGTGCCATACA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGTGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGATGACTAAAct < 1:12130/250‑2 (MQ=255) gATGGCGTCAAGAACTCTGACTGCAGGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCGGAGAGATCGCAGGTTGACTCAAAGTAAGGGTTTCAGCCGCGCTCCCCAAAGTTCCGTGTTGTGCGACATCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGTCtttttttt < 2:302037/250‑1 (MQ=255) aaGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGATCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCTCTGACTAAACAGACTATTTTTTTCCATAAGCg < 1:115035/251‑1 (MQ=255) aCTGCATGCGGTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTa < 2:89639/216‑1 (MQ=255) aCTGCACGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTa > 1:89639/1‑216 (MQ=255) gtaagTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTg < 1:74215/251‑1 (MQ=255) ccACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTc > 1:112286/1‑209 (MQ=255) ccACGCTCAAACAAGCGAGCGCCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTc < 2:112286/209‑1 (MQ=255) gcgcCATTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTaa < 1:295609/251‑1 (MQ=255) | TGTTGCTGATGAAACTGACCTATAACCGAAGGTAATATCCCCAGTGCCGCAGTAGGTAGTGCACCAACCCTGACGACATCATTATTAAGACCTTCTTTACGATGAAGCGACCGTCCGGCAGTGTTGATGGCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCA‑GCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAA > NC_000913/1494692‑1495158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |