Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,339,315	C→T	intergenic (‑155/‑15)	ribA ← / → pgpB	GTP cyclohydrolase 2/phosphatidylglycerophosphatase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,339,315	0	C	T	100.0%	66.5 / NA	22	intergenic (‑155/‑15)	ribA/pgpB	GTP cyclohydrolase 2/phosphatidylglycerophosphatase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/14);  total (8/14)

CAGTTCTTCAAATCCCACCATCAGGAAATCGCCCCATGGGGTTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCTGCGTTTTCGCATTAAGGCTGCCTT  >  NC_000913/1339083‑1339556                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  cAGTTCTTCAAATCCCACCATCAGGAAATCGCCACATGGGGTTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGc                                                                                                                                                                                                                                 >  1:255783/1‑251 (MQ=255)                              cGCCCCATGGGGTTGGCAGTTTTGCTTCTGCCACACGTTTAAGCTGCATTAAAGTCTTCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCGGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTggg                                                                                                                                                                                                     <  2:239228/250‑1 (MQ=255)                                          ttGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTg                                                                                                                                                                                        >  2:179645/1‑251 (MQ=255)                                                   tGGCTTCTGCCCCCCGTTTAAGCTGCATGAAATTCTCCAGAGAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCAt                                                                                                                                                                                <  2:184195/250‑1 (MQ=255)                                                             cacaCGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTAGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTTTCATGCCAGTAGCCg                                                                                                                                                                     <  1:228718/251‑1 (MQ=255)                                                                                     ctcCAGCTAATGCTGGATCTGTATTGGCTTATTTTCCCATAACGAGACGGGGTTACCCTAATCACGCAGCGTCGATCGTTCACGGAATGGCACAAATCTGTCCATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGATGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTCTGGCTTTCTGGCTGGCGTTGGc                                                                                                                                             >  2:277041/1‑251 (MQ=255)                                                                                                gCTGGTTCTGTATTGGCTTAGTGTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAAGTTTTCCTGGAACGGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGaa                                                                                                                                  <  1:284113/251‑1 (MQ=255)                                                                                                 cTGGTTTTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAAc                                                                                                                                 >  1:86440/1‑251 (MQ=255)                                                                                                    gTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACaa                                                                                                                               >  1:2031/1‑250 (MQ=255)                                                                                                      tCTGTATGTGCTTATTTGGCAATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGGTCACGGAATGGCACAAATCTGTCAAGTTTTCCGGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTAGTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGGACCGAAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAg                                                                                                                             <  2:12179/250‑1 (MQ=255)                                                                                                         gTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGt                                                                                                                            <  1:5908/248‑1 (MQ=255)                                                                                                          tATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGc                                                                                                                        >  1:207421/1‑251 (MQ=255)                                                                                                                                      gggTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACGGGCGTTTTCAGTTATTATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGTAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGa                                                                                            <  2:86440/251‑1 (MQ=255)                                                                                                                                                       acgCGTCGATCGTTCCCGGAATGGCACAAATCTGGCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGGTGGACTTATTAAAAAGGAGAGGCCATTCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGTGTCTTTTGGGGTTACTGAAACTGTCACCCAGCCCt                                                                           <  2:155749/251‑1 (MQ=255)                                                                                                                                                       acgCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGGTGGCAACCTGGAGAACAAAGTTGGCTGCTAAAAGCGGCTTTTTGGGGTACTGAAACTGGCACCCAGCCCt                                                                           <  1:277041/251‑1 (MQ=255)                                                                                                                                                         gcgTCGATCGTTCACGGAATGTCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCGGCACTAGTGCGTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAGAGCGGCTTGTTGGGTTACTGAAACTGTCACCCAGCCCTgg                                                                         <  2:19400/251‑1 (MQ=255)                                                                                                                                                           gTCGTTCGTTCATGGAATGGCACAAATCTGTCAATTTTTCCGGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTTTTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTCCTGAAACTGTCCCCCAGCCCTgggg                                                                       <  2:279396/251‑1 (MQ=255)                                                                                                                                                               aTCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTc                                                                   <  1:195873/251‑1 (MQ=255)                                                                                                                                                                                        gTCAATTTTTCCTGGAACTGGCGTTTTCATTTATGATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTTATTTTATTc                                              <  2:255783/247‑1 (MQ=255)                                                                                                                                                                                                                          gATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCTg                         >  1:52532/1‑234 (MQ=255)                                                                                                                                                                                                                          gATTGTGGGACTTATTAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCTg                         <  2:52532/234‑1 (MQ=255)                                                                                                                                                                                                                                gggACTTATTAAAAAGGAGAGGCCATGCGTTCGCTTGCCAGACGTACCGCAGTGGGCGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCTGCGTTTTCGCATTAAGGCTGCCtt  >  1:300932/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  CAGTTCTTCAAATCCCACCATCAGGAAATCGCCCCATGGGGTTGGCAGTTTGGCTTCTGCCACACGTTTAAGCTGCATGAAATTCTCCAGATAATGCTGGTTCTGTATTGGCTTATTTTGCCATAACGAGAAGGGGTTCACCTAATCACGACGCGTCGATCGTTCACGGAATGGCACAAATCTGTCAATTTTTCCTGGAACTGGCGTTTTCAGTTATGATTGTGGGACTTATCAAAAAGGAGAGGCCATGCGTTCGATTGCCAGACGTACCGCAGTGGGAGCTGCACTATTGCTTGTCATGCCAGTAGCCGTATGGATTTCTGGCTGGCGTTGGCAACCTGGAGAACAAAGTTGGCTACTAAAAGCGGCTTTTTGGGTTACTGAAACTGTCACCCAGCCCTGGGGCGTCATTACACATTTGATTTTATTCGGCTGGTTTCTCTGGTGTCTGCGTTTTCGCATTAAGGCTGCCTT  >  NC_000913/1339083‑1339556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑