Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,674	G→A	G282R (GGG→AGG)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,674	0	G	A	92.0%	56.5 / ‑5.4	25	G282R (GGG→AGG)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (12/11);  minor base C (2/0);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAA  >  NC_000913/1336447‑1336898                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 aTACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGAAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTCTCCCGGATGTAGTGGGCTTCAAACTTACCGGACAATTACGTGAAGGTATTACGGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACACGGCGTGGTGAGGAAATTCGTCGAATTTTATggg                                                                                                                                                                                                           >  2:64128/1‑250 (MQ=255) aTACACACGTTGGTACTGACTCCCACACCACCATGCTCAACGAACTTGGCGTGCTGGGGCGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCCGCCGGTTTCCCTGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATgg                                                                                                                                                                                                            >  2:133508/1‑250 (MQ=255)        ggTTGGTCCTGCCCCGCACCCCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGGGTTGGTGGGATCGAGGCAGAAGCCGCAATGTTAGTCCAGCCGGTTTCCATGTTTATCCCGGATGTAGTGGGTTTCAAACTTACCGTAAAATGACGGGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCTCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTc                                                                                                                                                                                                    <  1:133508/250‑1 (MQ=255)         gttgttACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGACGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTAGTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGCGGTGCAGACATTCGTCGACTTTTCTTGTGATGGTCt                                                                                                                                                                                                   >  2:107270/1‑251 (MQ=255)                ctgactcgcacgaaaccacaaTCAAAGGCATTGGCGTACTGGGGTGGGGCGTTGGAGGGATCGCAGCAGAAGCCGCAACGTGAGGCCAGCCGGTTTCCATGCTTATCCCGGATGAAGTGGGCTTCAAACTTACCGGAACATTACGTGAAGGGATTACCGCCACAGACCTGGTTCTCACTGTTACCCCAATGATGCGCCAACATGGCGAGGTGAGGAAATTCGTCGAATTTTATGATGATGGTCTGGAtta                                                                                                                                                                                             >  2:289078/21‑249 (MQ=255)                  gACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACt                                                                                                                                                                                          >  1:309176/1‑251 (MQ=255)                                                 gttggttggggggggtggttgttGGGTTCGAACCAGAAGCGGCAATGTAAGGCCAGCGGGTATCCGTGCTTGCCCCGTAGGGAGGGGGCTTCAATCTAACCGGAAAATTTCGTGAAGGTATTACCGCCACAGACCTGTTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGAGGGTCGTGATGCGCGACCGTGCGCGGATCGCGCTACCATTGCCAat                                                                                                                                                           <  2:312924/234‑1 (MQ=255)                                                  cGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTACCCCGGTTGTAGTGGGCTTCAAACTTTCCGGAAAATTCCGTGAAGGTATTCCCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCGGGATTCTCTACCGTTGGCGGATCGCGCCACCATTGCCAata                                                                                                                                                          <  1:138957/251‑1 (MQ=255)                                                         gggtggggCGTTGGTGGGCTCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGAGGTGAGGAAATTCGTCGAATTTGATGGAGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCACTATGTCGcc                                                                                                                                                   >  2:60809/1‑251 (MQ=255)                                                          ggtggggCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATATCGCCa                                                                                                                                                  <  1:87741/251‑1 (MQ=255)                                                                gcgttgttgggctcgacgaagaccgcGCATTGTTAGGCCGGCCGGTCTCCATGCTTACCCCTGATGTTGTGGGCTTCAACCTCTCCGGAAAATTACGTTAAGGTATGACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCTCCATTGCCAATATGTCGCCAGAatat                                                                                                                                            <  2:211008/226‑1 (MQ=255)                                                                    tggtggGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTg                                                                                                                                        >  1:100743/1‑251 (MQ=255)                                                                    ggtggggATCAAAGCAGAAGCGGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCAGGATGTAGTGGGCTCCAAACGTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGTTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCTAGAATATGGTg                                                                                                                                        <  1:243119/247‑1 (MQ=255)                                                                                                          cGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCCATGCTGTAACCCTc                                                                                                  >  2:284092/1‑251 (MQ=255)                                                                                                                     cTTATACCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCg                                                                                       >  2:247601/1‑251 (MQ=255)                                                                                                                        aTCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTCCCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGttt                                                                                    <  1:15264/251‑1 (MQ=255)                                                                                                                            cGGATGTAGTGGGCTTCAAACTTACCGAAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGGCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGc                                                                                <  1:281042/251‑1 (MQ=255)                                                                                                                                            cAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATc                                                                <  1:50425/251‑1 (MQ=255)                                                                                                                                                       gAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAACTTCGTCGCATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTg                                                                                                                                                                >  2:149614/1‑144 (MQ=255)                                                                                                                                                       aaaaaTTTCGTGAAGGTATTACCGCCACAGACCTGTTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTCATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTg                                                                                                                                                                <  1:149614/143‑1 (MQ=255)                                                                                                                                                           attaggtgaagtatttACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATGCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTTTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCg                                                 <  2:22131/237‑1 (MQ=255)                                                                                                                                                                   aaGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAatat                                         >  1:46846/1‑251 (MQ=255)                                                                                                                                                                              gCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGCATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGCTCGCGCCACCATTGCCAATATGTCAACAGAATATGGCGCCACCTGTGGCTTCTTCCCAATCGATGCTGGCACCCTCGATTACATGCGTTTAAGCGGGAGCAGCGCAGATCAGGTCGAGTTGGTCGAACAATATGCCAAAgcgc                               >  2:196147/1‑250 (MQ=255)                                                                                                                                                                                   aGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGAGTGGTGCGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGc                          >  1:205275/1‑250 (MQ=255)                                                                                                                                                                                                           aaTGCTGCGCAAACATGGCGTGGAGAGGAAATTCGTCGAATTTTATGGAGATGGACTGGTTTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGACACAGCGCAGGGCATGGGGCGTAACCCGGGCGATgca  >  2:232903/1‑248 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 ATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAA  >  NC_000913/1336447‑1336898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑