Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	42.8 / NA	19	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/13);  total (6/13)

TGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAA  >  NC_000913/1306500‑1306959                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                gggggACCTATGATGAGGTCTCCCACAAACCACTACATCCTTACACCAGGGCATTAATGGCGGCAGTCCCCATTCCTGATCAGGATCTGGAGAAGAACAAAACCATCCAGTTGCTGGAAGGGGAATTCCCGTCGCCGAGCAACCCGCCTTCCGGTTGTGGTTGCCGTGCCCGTTGCCCGATTGCCGGTCCAGAGTGCTCCAAAACACGTCCTGTCCTGGAGGGGAGTTTCATACACGCCGTTTCTTGCCTg                                                                                                                                                                                                                   <  2:9280/250‑1 (MQ=255)  ggggACCTATGATGAGGTCTACCCCAATCCACTACATCCTTACTCCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTTTGTAGGGGAGTTTCAGACACGCCGTTTCTTGCCTg                                                                                                                                                                                                                   <  1:239736/250‑1 (MQ=255)                                          tACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCAACCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCTTGGCCCGATGGCCGTTCCAGAGTGCGCCAAACCACGTCCGGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAATTCGATCCGCTTTAAAAACAAGAAGGGCTGACAGTTg                                                                                                                                                                           <  1:108137/250‑1 (MQ=255)                                          tACACCAGGGCATGTATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGAGCCGCTTTAAAAACAATAAGGGCTGACAGTTg                                                                                                                                                                           <  1:31620/250‑1 (MQ=255)                                           acacCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCAGTACCCGTTGAACGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCAGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCAGACAGTTGTc                                                                                                                                                                         >  1:208974/1‑251 (MQ=255)                                                    cgtccgagctggCAGGCCCCATACCTGGTCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTGCCGGTTGTGTTTGCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTGTCCTGAAAGTCGATCCGCTTTAAAAACAATAAGTGCTGACAGTTGGCAGCCCtt                                                                                                                                                                  <  2:301024/239‑1 (MQ=255)                                                    cATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGGCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtttt                                                                                                                                                                <  1:183200/251‑1 (MQ=255)                                                        gATGTCGGCAGTCCCCATACCTGAGCCGGATCTGGATAAGACCAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTGGTCAGCCCTTTTTCa                                                                                                                                                             <  1:226611/250‑1 (MQ=255)                                                              ggCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGACGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGCGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtt                                                                                                                                                                  >  1:301024/1‑239 (MQ=255)                                                                                                            cAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGa                                                                                                                                                                                                                  >  1:74336/1‑145 (MQ=255)                                                                                                            cAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGa                                                                                                                                                                                                                  <  2:74336/145‑1 (MQ=255)                                                                                                                 ccgggaagtggattgaCCGTCGCCGTGCACCCCGCCTGCCGGTGGGGTTTTCCGTACCCGTTGCCCTATGTCCGGTCCAGAGGGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTAAGACACGCCGTTTCTTGCTTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGTCCTTTTTCACGCTAAAAGCGATTATTGAGTCCCGCCAGATGATATGGCAAAGTTTGTTATtttttt                                                                                                    <  2:181755/235‑1 (MQ=255)                                                                                                                                             aaccaaccTTCCGCTTGTGTTTTCAGTACACGTTGCCCGATAGCCGGTACAGCGCGCGCCCAAACACGTCCTGATATGGAGGGGAGCTTCCGCCCCGCAGTTTCTTGCCTGAAAGTCGAACCGCTTTAAAAACAATACGGGCTGACAGTCGTCAGCCCTTTTTCACGCTACACGCGATAATTTATTCCCGCCAGATGATATGGCAACGATTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAAc                                                                        >  2:147976/7‑250 (MQ=255)                                                                                                                                                          gTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAc                                                          >  1:227747/1‑251 (MQ=255)                                                                                                                                                            tgtgTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTTCGCCAAAACACGGCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCa                                                                                                                                                                        <  2:95018/139‑1 (MQ=255)                                                                                                                                                            tgtgTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTACTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCa                                                                                                                                                                        >  1:95018/1‑139 (MQ=255)                                                                                                                                                                        ccccGTTGCCCGATGTCGGGTTCAGAGAGCGCCAAAACTCGTCCTGTTCTGGAGGGGATTTGCAGACACGCCGGTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATGCCCGCCAGATGTTATGGCAAAGTTTTTGATCTTTTTCGCGGCATATCAGAATGCGGGCAAAAACATCGTTGATCTCACCATCGTCACTGTc                                             <  2:152900/249‑1 (MQ=255)                                                                                                                                                                                    aTTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATc                                <  2:89550/251‑1 (MQ=255)                                                                                                                                                                                                                  ccttttttgggggggTGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCaaa  <  2:264360/240‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                TGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAA  >  NC_000913/1306500‑1306959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑