Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,148	T→A	Y84K (TAT→AAA)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,148	0	T	A	100.0%	30.5 / NA	11	Y84K (TAT→AAA)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/5);  total (6/5)

AGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGG  >  NC_000913/1302931‑1303386                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               aGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATAAATCGGTCAATGACTTGGTTGCATCCAGTTTTc                                                                                                                                                                                                               >  1:305628/1‑251 (MQ=255)              cTATTTATTCTTATTACTATTTAGTTATTTATGATGCGCCTCGCGCCGGGACGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGAAACAGTATTTCAGCTACCTGAAACAAATGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATAAATCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCa                                                                                                                                                                                                   >  1:125468/1‑250 (MQ=255)                atttattCTTATTACTATTACGGTCTTAATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGTCGAACGTACTTTCCCGCCAGAAGTGATGGTCAATATCGAAGCGAAATATCATCGTAATGATCCGATCATGACACAGTATGTCAGCTACCTTAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAATTATAAATCGGTCAATGTCTTGGTTGCATCCATTTTTCCCGTTTCTTCCaaa                                                                                                                                                                                                 <  2:125468/250‑1 (MQ=255)                           ttaCTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTCAATATAAAGCTAAATCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCgc                                                                                                                                                                                      >  2:85873/1‑250 (MQ=255)                                agtgcGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTGACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATAAATCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCgcagca                                                                                                                                                                                  <  2:305628/245‑1 (MQ=255)                                     gTTCTTTATGATGCGCCTCGCGCCGGGAAGCTTTTTCACCGGCGGACCCACTTTGCAGCCAGAAGTGATGCCCAATATCGAAGCGAAAAACAATCATAATGATCCGATCATGACACCGAATTTCAGCTACCTGAAACAACTGGCGCACGGAGATTTCGGTCCATCGTTTAAATATAAAGATAAATCGGTCACTGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCACACTGGGAGACGCAGCATTTTTcc                                                                                                                                                                           >  1:193429/1‑251 (MQ=255)                                                                                                                           aaTATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATAAATCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCACACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATAcc                                                                                                    >  2:205753/1‑236 (MQ=255)                                                                                                                           aaTATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATAAATCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATAcc                                                                                                    <  1:205753/236‑1 (MQ=255)                                                                                                                                                                                 tGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATAAATCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGat                                >  1:9053/1‑250 (MQ=255)                                                                                                                                                                                    cgcACGGTGAGTTCGGTCCATCGTTTAAATATAAAGATAAAGCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCTCCATTATTAGTCATGATAttt                            <  2:96260/251‑1 (MQ=255)                                                                                                                                                                                                              aaaTATAAAGATAAATCGGTCAATGACTTGGTGGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACTCCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCAGTATTAGTCATGATATTTGCTATCATTTTGCATTGGCTGCCggg  <  1:115562/251‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               AGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAAAACAAAACACCAAATGGGACTATACCGTGATGGGGCTGGCAATGACCGGGGTTGTTATCCCCAGTTTTGTGGTTGCACCATTATTAGTCATGATATTTGCGATCATTTTGCATTGGCTGCCGGG  >  NC_000913/1302931‑1303386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑