Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,291,196	T→C	W319R (TGG→CGG)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,291,196	0	T	C	100.0%	80.6 / NA	24	W319R (TGG→CGG)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

AGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACAC  >  NC_000913/1290958‑1291435                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                agagTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGGCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCaaa                                                                                                                                                                                                                                     <  2:310524/251‑1 (MQ=255)                  tGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCggtggt                                                                                                                                                                                                                    >  1:268148/1‑251 (MQ=255)                          gTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTgcg                                                                                                                                                                                                            >  2:298786/1‑251 (MQ=255)                                                                  ttCCGCTTTTTGTTGGCTATTAGCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGTGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTTCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACTCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCTGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCggg                                                                                                                                                                     <  2:1786/250‑1 (MQ=255)                                                                                    attatCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTATGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAAGATGGGGAACCggt                                                                                                                                                                                                                       <  2:301537/182‑1 (MQ=255)                                                                                    attatCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATCCGCCGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCggt                                                                                                                                                                                                                       >  1:301537/1‑182 (MQ=255)                                                                                         catcGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCCGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGCACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATg                                                                                                                                             >  1:86848/1‑251 (MQ=255)                                                                                            cgcgAACTGAAAAATCTCAGTCTGGGTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCg                                                                                                                                          <  2:172420/251‑1 (MQ=255)                                                                                              cgAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGAtg                                                                                                                                                                                                     >  2:57308/1‑190 (MQ=255)                                                                                              cgAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGAtg                                                                                                                                                                                                     <  1:57308/190‑1 (MQ=255)                                                                                                          tctcATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGACGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGCATCAATTGAGCCAGCGATGAGATGCCCGGCACTGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTAt                                                                                                                            >  1:211906/1‑251 (MQ=255)                                                                                                             cATTCTGGTTTCTGCGGGTCTGAATTCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCTCTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCTATGCCAAATATGGGGAACCTGTGGGCGACTGCGCGTTATGTTGTCTGCGTAATGAGCAAACGAGAACGCTGGCTAAATTTGCATTGCCTTCTTATGTCGGCTGGTGGTACTAtc                                                                                                                           <  2:211906/249‑1 (MQ=255)                                                                                                                  tGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCTATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGcc                                                                                                                     >  1:218389/1‑250 (MQ=255)                                                                                                                    gTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCAtt                                                                                                                  <  1:86859/251‑1 (MQ=255)                                                                                                                    gTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGATTATTTGAATCAATTGAGCAAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCAtt                                                                                                                  >  1:303195/1‑251 (MQ=255)                                                                                                                          gCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGGACTATCGTCGCCATTCGtat                                                                                                             <  2:119517/250‑1 (MQ=255)                                                                                                                                cTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAAt                                                                                                      >  1:92100/1‑251 (MQ=255)                                                                                                                                                             ccAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGTCTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCTATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCTTCCttt                                                                         <  1:51089/251‑1 (MQ=255)                                                                                                                                                                           gTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGACATGCTAAATATGTGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTCAATTTGCATTCCCTGCTAATGTCGTCTGGTGTCACTATCGTCGCCATTCGTTTAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGacattagtcc                                                           >  2:293246/1‑242 (MQ=255)                                                                                                                                                                                                        accGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCCGGCAATGCCAACTATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAAAGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCATTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCg                               >  2:232332/3‑250 (MQ=255)                                                                                                                                                                                                                           tGAGCCAGCGATGCGACGCCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACg            <  1:10837/250‑1 (MQ=255)                                                                                                                                                                                                                             aGCCAGCGATGCGATGCCCGGCAATGCAAAATATGGGGAACCGGGGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACTATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGAGATACAGGGATGCGATACAGAAATATGAACACGTTc         <  1:77063/251‑1 (MQ=255)                                                                                                                                                                                                                                    gatgcgatgcCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCATAAGGAGCAAACGATAACGCGGGCTAAATTTGCATTTCCTGCTAATGTCGGCTGGTGGTGCTATCGTCTCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTc                                                                       <  1:316410/182‑1 (MQ=255)                                                                                                                                                                                                                                    gatgcgatgcCCGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAacac  >  1:116865/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                AGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGGTGGTACTATCGTCGCCATTCGTATAAGTAATTGTCTTAATTATGCTAACTCGCCTCCTTTTCAGAACTTAGCCCCTTCGGGGTGCTGATATACTGGGATGCGATACAGAAATATGAACACGTTCAAAACAC  >  NC_000913/1290958‑1291435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑