Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,040	G→A	intergenic (+56/+26)	ychS → / ← rttR	putative uncharacterized protein YchS/small RNA RttR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,040	0	G	A	100.0%	13.0 / NA	8	intergenic (+56/+26)	ychS/rttR	putative uncharacterized protein YchS/small RNA RttR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

CCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA  >  NC_000913/1286821‑1287256                                                                                                                                                                                                                            |                                                                                                                                                                                                                         ccTTCCCCGCTTGGCCAGAATACTTGACTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTCCCCGCTTCACCCTTCGGGTTGGTGCCTGCGGCACCGCTCTCTCGCTGACGCTCGAGCCGCAACTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGACTATTTTCTTGGGGATTAGCTTAAGAGTTCAGGGCCTTTTgcgcgtgatggcgg                                                                                                                                                                                           >  1:59560/1‑238 (MQ=18)                                   ttCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCTCTTCGc                                                                                                                                                         >  1:303322/1‑251 (MQ=255)                                                  cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGc                                                                                                                                          >  1:75302/1‑251 (MQ=255)                                                    gggCTTTGGAAAGTGATGGGGGTGGGGGGAGGATTACTCAGCCCGGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGCTCTCGCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTACCCCGGATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                                                                                                                                        <  2:75302/251‑1 (MQ=14)                                                                                                                   gTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATCTTTAATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGCAAGTGATGGTGGTGGGG‑AAAGAATACTCACCGCTACGCGCTTCGACCTCCGGGTTCTTGCCTGCGGCCACGCTCTCTCTCCGGAGCTCGGATCGAAACTTGGCTGAAGCTCCTCCTCCTTCCCCGc                                                                        >  1:14914/1‑251 (MQ=17)                                                                                                                        tGCCTGCGGCAACGCTCTCTCGCTGACGATCGAGCCGAACCTTAGTCGAAGCTTCGCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGATTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCACGCATg                                                                     <  1:250376/250‑1 (MQ=255)                                                                                                                                                              accggggtcaaaGCTTCTCCTCCTTCACCGTATGGGCAGAATATTTGATTGCGGATTCGTTTAAGAGTTCAGGGATTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                              <  1:175139/241‑1 (MQ=17)                                                                                                                                                                                          cgcgtGGGCAGAATATGTGATTGCGGATTCGTTTAAGTGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCTAACCTTGGTCGAAGCTTCTCAGCCTTCCCCGCATGGGCAGAATATGTAAGTGCGGATTCGTTGTGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGa  <  1:302852/247‑1 (MQ=14)                                                                                                                                                                                                                            |                                                                                                                                                                                                                         CCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA  >  NC_000913/1286821‑1287256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑