Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,189,980	A→G	T156T (ACT→ACC)	phoP ←	DNA‑binding transcriptional dual regulator PhoP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,189,980	0	A	G	100.0%	79.2 / NA	23	T156T (ACT→ACC)	phoP	DNA‑binding transcriptional dual regulator PhoP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/9);  total (14/9)

AGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGC  >  NC_000913/1189739‑1190200                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                             aGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAAGTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTGTGCCATTAGTGCGTATCAACGTTTCCAGAATGGTGTATTCg                                                                                                                                                                                                                     <  2:145337/251‑1 (MQ=255)   cGAGAGCGGGAAACAAAGAAGCAGTAATTTTTTCATCAGCGCAATTCGAACCGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGCTATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCgc                                                                                                                                                                                                                    >  2:313946/1‑249 (MQ=255)       agCGGGAAAAAAAGACGCAGTAAGTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAAcgcg                                                                                                                                                                                                               <  1:313946/251‑1 (MQ=255)                                 tttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAAAGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAAt                                                                                                                                                                                     >  1:23791/1‑251 (MQ=255)                                        aGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGAAATCACTTCTTGGCGCTATTGTGCCTGAATTTTTTTGCGCAGCCGTCCCCTCACTACCTCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAAt                                                                                                                                                                              >  1:132932/1‑251 (MQ=255)                                        aGCGCAATTCGAACAGATAGCCCTGGCCGCGAAAGGTGGAAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGAATGGTTTTCCCGCAGCTACGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTCTTCGACCGCGGTCAGTTTGATCACTTCGTCATTAATAGAGAAt                                                                                                                                                                              >  1:283584/1‑251 (MQ=255)                                                tCGAACAGATAGTCCTGGCCGCGAACGGTGGTAATCACGTCGTGGGGATATTGTGCCTGAATTTTTTTGCGCAGCCGTCCCATCAGTACATCAATGGTATGGCTTTCCCTCAGCGCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATGCGAACGCGGGCCGGTTGATCACTTCGTCAtt                                                                                                                                                                                        <  2:126992/233‑1 (MQ=255)                                                tCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGGGTATTCGAACGCGGTCAGTTTGATCACTTCGTCAtt                                                                                                                                                                                        >  1:126992/1‑233 (MQ=255)                                                        aTAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATGCGATCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGATAGAGAt                                                                                                                                                              <  1:254607/251‑1 (MQ=255)                                                        aTAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGAGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGAAAATCAATGGAATGGATTTCCCGCAGCTCCGAATCCGGATAGAATTGGAGCATTAACGACTCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCgagag                                                                                                                                                                  >  1:140235/1‑247 (MQ=255)                                                          aGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGCGATc                                                                                                                                                             >  2:32057/1‑250 (MQ=255)                                                                          ggtggtAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACgggggg                                                                                                                                             >  1:65587/1‑250 (MQ=255)                                                                             ggtAATTACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTAAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGGGCg                                                                                                                                         >  1:93279/1‑251 (MQ=255)                                                                                   cACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCCATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTCACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGTTCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAAACTGAAACGGGGGGAGCGACATg                                                                                                                                    >  2:47369/1‑250 (MQ=255)                                                                                                 atTGTGCCTGAATTTTTTTGCGCAGACGGCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACGTTGCCATTATTGCGTATCAACGGTTCCATAATGGTGTAGTCGAACGCGGTCAGTTTGAGCAGGTCGTCAGGAATAGAGAATTCACGGCGAGAGAGATCAGCCTGATACGTGGGGGGCGTAATGGCCTGTGAGGCCAg                                                                                                                      <  2:132932/250‑1 (MQ=255)                                                                                                           aaTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTc                                                                                                                                                                                                                      >  2:280559/1‑144 (MQ=255)                                                                                                           aaTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTGTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTc                                                                                                                                                                                                                      <  1:280559/144‑1 (MQ=255)                                                                                                                   tGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCAACCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCTGACCGCTATTCCGCAGCAt                                                                                                    <  2:93279/250‑1 (MQ=255)                                                                                                                                                                                     ggAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCGTCAATATTAAACGGTTTAGTCACATAATCATCAGCAcc                                  <  1:274749/250‑1 (MQ=255)                                                                                                                                                                                                         cTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACttt             >  1:87543/1‑251 (MQ=255)                                                                                                                                                                                                                    gCCATTATTGCGAATCAACGTTTCAATAATGGTGTATTCTAACGCGGTCAGTTTGATAACTTCGTCATAAATAGCTAATTAACGGCGAGAGAGATCAAACTGAAACGGGGGGAGAGAAATGACCTGTGAAGCCAGACCGCTATTAAGCCGCATTAATGCCTGAATTCGCCCCATCACCACTTCAATTTGAAACGGATTAGTCACATAATCTTAATCACCGGCCCTTAATACTTAGACATTGTACTGCAAGc  >  2:187639/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAatcat                                         <  1:94266/191‑1 (MQ=255)                                                                                                                                                                                                                                         ttCCATAATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAatcat                                         >  2:94266/1‑191 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                             AGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGC  >  NC_000913/1189739‑1190200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑