Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	31.8 / NA	12	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/3);  total (9/3)

GCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCG  >  NC_000913/1169614‑1170074                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               gcACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATCTTCACTTTGGTGCCTGGGGAGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGCACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGAAACTc                                                                                                                                                                                                                    >  2:241317/1‑251 (MQ=255)                            aTCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGcgcg                                                                                                                                                                                         >  1:65022/1‑251 (MQ=255)                                       gatgatATTCACTTTGGTGCCTGGGTTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGcc                                                                                                                                                                               <  2:57092/250‑1 (MQ=255)                                             atttaCTTGGGTGCCTGGGGGGACCTGGCTAACGAGTGTTTTGAAATCGTCAGCCAGCAGACGAATCCAGCCAGAACTTACCCGCATGCCAAGGCCGAAATCTGCGTTCGGACTATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGGTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTGTATAACGGGCGCGGATGTTTGCCGTTGGc                                                                                                                                                                         <  2:24769/246‑1 (MQ=255)                                                   tttGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGACGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGAGGATGTTTGCCGTAGGCGTCCAgg                                                                                                                                                                  >  1:251081/1‑251 (MQ=255)                                                                                       gATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCAcc                                                                                                                               >  1:100919/1‑250 (MQ=255)                                                                                                aTCCCGCAGAAGAATACAGCCAGCACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCACATAAACGCCGCCATAGCCCGCCAGACGAATCGCCTGATGGCCAATTGGGTTATCCGGTCCACCCGGCACTACCACAGGCAACTCAATTCCCGTTGCTTTCTAACGGGCGCGGATGTTTTCCGTTGGCGTCCCGGGTTGGTTGGCACGGTTGCCTGAAACGGGGGTCACCATTGTCgctg                                                                                                                     >  2:245824/1‑248 (MQ=255)                                                                                                                    cAGAACTTACCCGCATGCCAATGCCGAAATCGGCGGTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGAATGATGGCCCATTGGGTTATCCGGTCCAGACGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGCTGATTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAAc                                                                                                 >  1:177439/1‑251 (MQ=255)                                                                                                                             cccGCACGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAAt                                                                                         >  1:86534/1‑250 (MQ=255)                                                                                                                                                                                               gaagaacggCATGATGGCCCAGTGGGTTTTCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTGATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTTGGTTGGCACGTTTTTCTGAAACGGTGGTCACCATTGTCGGTTTCAGCTTGTCACCACCTAACTGACCAATACCTAGTGGACACTTGGTTACCGAATTTTTACCCTTCGGGTAGTAATAGAGACTCAGATCCGCAATg                      <  2:177439/243‑1 (MQ=255)                                                                                                                                                                                                                cccATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTc     >  1:896/1‑251 (MQ=255)                                                                                                                                                                                                                   aTTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTcgcg  >  1:206015/1‑251 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               GCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCG  >  NC_000913/1169614‑1170074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑