Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,972	C→T	Q72* (CAG→TAG)	fabH →	3‑oxoacyl‑[acyl carrier protein] synthase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,972	0	C	T	100.0%	74.5 / NA	28	Q72* (CAG→TAG)	fabH	3‑oxoacyl‑[acyl carrier protein] synthase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

TATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAACCGATCGTGGG  >  NC_000913/1148731‑1149214                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   tatataACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGcc                                                                                                                                                                                                                                           >  1:79609/1‑251 (MQ=255)    ataACCGAAAAGTGCCTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTgt                                                                                                                                                                                                                                        >  1:82259/1‑250 (MQ=255)                                           tattGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCg                                                                                                                                                                                                  >  1:306344/1‑250 (MQ=255)                                           tattGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGCCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACAACTTCTGCTACGCACGCTTTccggg                                                                                                                                                                                                 >  1:5959/1‑248 (MQ=255)                                                      gCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGCGTGGATTGTCCCCCGTACCGGTACCCGCGAACGCCACACTGCCGGGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGATCGCAGCTTGt                                                                                                                                                                                      <  2:5959/251‑1 (MQ=255)                                                                gCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCGGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGAAGCTTGTCAGATTCaaa                                                                                                                                                                            <  2:306344/251‑1 (MQ=255)                                                                       cAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGtt                                                                                                                                                                     >  1:298355/1‑251 (MQ=255)                                                                          gTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTggg                                                                                                                                                                  <  1:310421/251‑1 (MQ=255)                                                                             cGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCAt                                                                                                                                                               <  2:82259/251‑1 (MQ=255)                                                                                                 aaaaaTGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTg                                                                                                                                           <  1:140904/251‑1 (MQ=255)                                                                                                    aaTGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACg                                                                                                                                        >  1:214091/1‑251 (MQ=255)                                                                                                                      cGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAg                                                                                                                      <  1:285426/251‑1 (MQ=255)                                                                                                                                             gTATCCGCGAACGCCACACTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAAGTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGGGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTa                                                                                               <  2:860/251‑1 (MQ=255)                                                                                                                                                     gAACGCCACATTGCCGCGCCAAACGAAACCGTTTAAACCATGGGATTTGAAGCGGCGACACGCGCAATTGAGATGGCGCGCATTGAGACAGAATAGATTGGCCTGATCGTAGTGGCAACGACTTCTGCTAAGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGATACAGCAGCCAGCGAAGGTTTCACCTATGCATTAAGCGTAGCCGATc                                                                                        >  1:100496/1‑250 (MQ=255)                                                                                                                                                      aaCGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGATACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAATGGTTGGCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCGTTAATCGTAGCCGATCa                                                                                       >  2:122136/1‑250 (MQ=255)                                                                                                                                                                  ccGCGCCAAACGAAGCCGTTTCATCCATGGGCTTTGAAGCGGCGTCACTCGCAATTGAGATGGCGGGCATTGAGAAAGACTATATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCTAGCGCTGCTTGTGAGATTCAAAGCATGTTGGTGATGAAAGTTTGCCCGGCATTGGACTTGGTAACGGCCTGCTCAGATTTCTCCGATGCATTAAGTGTAGCTGATCAAAACGTGAAATCt                                                                          <  2:28404/251‑1 (MQ=255)                                                                                                                                                                       ccAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGAGTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGc                                                                     <  1:1500/251‑1 (MQ=255)                                                                                                                                                                                aCCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCCTTGGGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTATGCTACGCAAGCTTTCCCGAGCGCAGCTTGTAAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACATATGCATTAAGCGTAGCCGATCAATAAGTGAAATCTGGGGAGGTGaaac                                                             >  2:221374/1‑248 (MQ=255)                                                                                                                                                                                      tCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGctct                                                      >  1:246967/1‑251 (MQ=255)                                                                                                                                                                                              gggCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTATATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGtcgtcg                                              <  1:253203/251‑1 (MQ=255)                                                                                                                                                                                                          ggcgACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGGATGCTCTTGTCGTCGGTTCCGATGTAc                                  >  1:86880/1‑251 (MQ=255)                                                                                                                                                                                                              acacGCGCAATTGCGATGGCGGGCATTAGGAAAGACTCGCTAGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCCCGCTTTCCCGACCGCAGCTTGTCAGATTCAAAGCATGTTGGGCCTTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTACGCGTAGACAATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGtta                                          >  2:314343/1‑238 (MQ=255)                                                                                                                                                                                                              acacGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGTCTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAATTCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTc                                          <  1:314343/239‑1 (MQ=255)                                                                                                                                                                                                                  gcgcAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATAGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCCGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAAACTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCCGTGTACTGgcgcgg                          >  2:207819/1‑250 (MQ=255)                                                                                                                                                                                                                         tGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGGCGTCGGTTCCGATGTACTGGCGCGCACCTGc                    <  1:25724/250‑1 (MQ=255)                                                                                                                                                                                                                               ggCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCa              <  1:177102/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACGGGCGCGCACCTGCGATCCAACCGATCg      <  2:180949/251‑1 (MQ=255)                                                                                                                                                                                                                                          agaAAGACTAGATTGGCATGATCGTTGTGGCAAAGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCCTGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGACATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGGTGTCCTGGCGCGCACCTGCGATCCAACCGATCGCggg  >  2:290503/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   TATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAACCGATCGTGGG  >  NC_000913/1148731‑1149214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑