Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	100,946	T→C	M61T (ATG→ACG)	murC →	UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	100,946	0	T	C	100.0%	144.1 / NA	44	M61T (ATG→ACG)	murC	UDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/20);  total (24/20)

GCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGC  >  NC_000913/100700‑101189                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gCCCGGGCGTAATTGTAGCGATGACTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAAt                                                                                                                                                                                                                                                 >  1:231437/1‑251 (MQ=255)     gggCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAAGACACAACAATTGGCAAAACTGCGGTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCCGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCTCGCAGCAGTTAACGAATCTgg                                                                                                                                                                                                                                             <  2:155562/251‑1 (MQ=255)               gTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGat                                                                                                                                                                                                                                   >  1:20852/1‑251 (MQ=255)                    gATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGatttatt                                                                                                                                                                                                                              >  1:184225/1‑251 (MQ=255)                                      tGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCCTCGCCCggca                                                                                                                                                                                                             >  2:81020/1‑248 (MQ=255)                                            tAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTATGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATATGGGTGCGACGATTTATTTCAACCATCGCCCGGCAAacgtac                                                                                                                                                                                                      >  2:105013/1‑251 (MQ=255)                                                 agTTAATGGCGTAAAGAATGAATTCACACCAATTGGCAAAACTGCGTTCCATCGTGTCCGAAATGCGTCGCGTTCGGCACATACATTGTGTCGGCATTGGTGGTGCCGGTATGGGCTGTATTGCCGAAGTGCTGGGCAATGAAGGTTAGCAGATGAGTGTTTCGGATTTATCGCCAAATCCGGTCACGCAGCGGTTAACGAATCTGGGTGTGGCTGGTTATTGCAACCATCGCCCgtattacttacgtgat                                                                                                                                                                                                 <  2:30257/251‑16 (MQ=255)                                                 agTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGAACGAAATGCGTCGCGTTCGGCACATCCATTTTGTCGGCATTGGTGGTGCCGGAATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGAt                                                                                                                                                                                                 >  1:99045/1‑251 (MQ=255)                                                     aaTGGCGTACAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCAGGTCTGGGCGGTATTGCCGAAGTTCTGGCCAATGACGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGcc                                                                                                                                                                                              >  2:257770/1‑250 (MQ=255)                                                      aTGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGCAAACGTACGTGATGcc                                                                                                                                                                                              >  2:66522/1‑249 (MQ=255)                                                           gTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTgg                                                                                                                                                                                       >  2:119749/1‑251 (MQ=255)                                                                 aatgaatACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCGGCGTGGGCgttg                                                                                                                                                                                 <  2:259305/251‑1 (MQ=255)                                                                      atACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGGCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTcc                                                                                                                                                                            <  1:221179/251‑1 (MQ=255)                                                                           caacaaTTGGCAAAACTGCGTTCCATCGTGCCCGTAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGCGCCGGTCTGGGCGGTATTGCTGACGTTCTGGCCAATGCAGGTTACCAGATCAGTGGCTCAGAATAAGCGCCAAATACGGACACGCAGCAGTTAACGAATATGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGGATGAGATGCGAGCGTGGACGTTGTTTCCAgcgc                                                                                                                                                                       >  2:44713/1‑251 (MQ=255)                                                                            aacaaTGGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATTCCAGCGTGGTCGGTGTTTCCAGcgcg                                                                                                                                                                      <  2:296970/251‑1 (MQ=255)                                                                              caaTTGGCAAAACGGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCAGCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGAt                                                                                                                                                                    <  1:165711/251‑1 (MQ=255)                                                                                   ggCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTTGTTTCGATTTAGCGCCAAATCCTGTCATGCAGCAGGTAACGAATCTGGGTGCGACGATTTATTTCAGCCGTCGCCCGGAAAACGTATGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCt                                                                                                                                                                <  1:289103/250‑1 (MQ=255)                                                                                      aaaaCTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCg                                                                                                                                                            >  1:117389/1‑251 (MQ=255)                                                                                          cTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATa                                                                                                                                                         >  1:202607/1‑250 (MQ=255)                                                                                            gCGTTCCATCTGGCCCGAAATGCGTCGCGTTCGGCACATTCATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCTAATGAAGGTGATCAGATCAGTGGTTCCGATTTAGCGCCAAAGCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTTTTTCCAGCGCTATTTCTGTCGATAAc                                                                                                                                                       <  2:33327/250‑1 (MQ=255)                                                                                            gCGTTCCATCGTGCCAGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAAcc                                                                                                                                                      >  1:71517/1‑251 (MQ=255)                                                                                              gTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCg                                                                                                                                                    >  1:239799/1‑251 (MQ=255)                                                                                                                  cGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGAATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGACTCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATg                                                                                                                                 >  1:308444/1‑250 (MQ=255)                                                                                                                        gTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGcgcg                                                                                                                          >  1:169466/1‑251 (MQ=255)                                                                                                                                       tttGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATcc                                                                                                            <  2:184225/250‑1 (MQ=255)                                                                                                                                        ttGTCGGCATTGGTGGGGGCGGTATGGGCTGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGATCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGGGCGGCGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATTCCAGGGTGGTCGTTGTTTCCAGCGCGATGTCTGCCGATAAGCCTGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcg                                                                                                           <  1:314366/250‑1 (MQ=255)                                                                                                                                            cGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGCAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAAACCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGCTCCGTCGTg                                                                                                      >  1:285595/1‑251 (MQ=255)                                                                                                                                                aTTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCGGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTGCGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATATCCCGGAAATTGTCGCCTCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGa                                                                                                  <  1:146755/251‑1 (MQ=255)                                                                                                                                                         gCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctgg                                                                                          <  2:223467/250‑1 (MQ=255)                                                                                                                                                            gggaTGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTCTCAGATCAGGGGGTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGAGCGACGCTTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTTCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGAATTACGGACATCCGTCGAGCCGAACTgctgggtg                                                                                       >  2:262974/4‑247 (MQ=255)                                                                                                                                                                 gggCGGTATTGACGAAGTTCTGGCAAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGCCGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTCTTCCTGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTa                                                                                  >  2:227494/1‑250 (MQ=255)                                                                                                                                                                         ttGCCGAAGTTCTGGGCAAGGTAGGTTATCAGTGCATTGGTTCCGCTTTAGCGCAAAATCCGGTCACGCGGAGGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGGGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGttt                                                                          <  2:210824/250‑1 (MQ=255)                                                                                                                                                                               aaGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCAt                                                                   <  1:66522/251‑1 (MQ=255)                                                                                                                                                                                aGTTCTGGCCAATGAAGGTTATCAGAGCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATg                                                                  <  1:60565/251‑1 (MQ=255)                                                                                                                                                                                   tCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCTTCATGGc                                                                <  2:81359/250‑1 (MQ=255)                                                                                                                                                                                            tGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTg                                                      >  1:189966/1‑251 (MQ=255)                                                                                                                                                                                                ggTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGAGTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCgg                                                  <  2:231437/251‑1 (MQ=255)                                                                                                                                                                                                          tcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGCTCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGc                                        >  1:92452/1‑251 (MQ=255)                                                                                                                                                                                                                   ccGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGAc                                >  1:271005/1‑250 (MQ=255)                                                                                                                                                                                                                               cAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCCGAGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAAc                                                                                                                                                       >  1:277084/1‑119 (MQ=255)                                                                                                                                                                                                                               cAAATCCGGTAACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGGCGTTGTGGCCATCGCGATTTCTGCTGATAAc                                                                                                                                                       <  2:277084/119‑1 (MQ=255)                                                                                                                                                                                                                                     cGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGGCGGTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTTGCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATTGCATCGCCATTGCCGGAACTCACGGCAAAACGACAACCACCGCGATGGTTTcc             <  1:179906/251‑1 (MQ=255)                                                                                                                                                                                                                                              agcagTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTAc    <  1:156479/251‑1 (MQ=255)                                                                                                                                                                                                                                                cagTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAACACGACAACCACCGCGATGGTTTCAAGCATCTACGc  >  2:269541/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTACGC  >  NC_000913/100700‑101189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑