| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,043,367 | C→T | E282K (GAG→AAG) | etk ← | protein‑tyrosine kinase Etk |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,043,367 | 0 | C | T | 94.1% | 112.5 / NA | 34 | E282K (GAG→AAG) | etk | protein‑tyrosine kinase Etk |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (19/13); minor base G (0/2); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATTCAGGCGTTTGCGTTCTTGCTCCAGCGTCTGGCGTTTTTCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTCGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTAAAGGTTTCCTGCAATGCGTTAATCGCTTCCAGTTCGGTACGCTGGCTC > NC_000913/1043132‑1043605 | ttATTCAGGCGTTTGCGTTCTTGCTCCAGCGTCTGGCGTTTTTCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAgg > 1:101562/1‑251 (MQ=255) gCGTTCTTGCTCCAGCGTCTGGCGTTTTTCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTa < 1:233987/251‑1 (MQ=255) gCTCCAGCGTCTGGCGTTTTTCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTc < 2:278767/250‑1 (MQ=255) tGGCGTTTTTCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGACTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTtgtg > 2:264070/1‑250 (MQ=255) ggCGTTTTTCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGa > 1:255728/1‑250 (MQ=255) tttCCATCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCATCGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGGTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACTTAGAGTTTTTCTTCCTCTTGGTCCATCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAATAATTCAATGCTGTGTGAATCCTg < 2:198570/250‑1 (MQ=255) tCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAg > 1:125408/1‑225 (MQ=255) tCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGGTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTGCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAg < 2:125408/225‑1 (MQ=255) cagcGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCt > 1:8200/1‑251 (MQ=255) aCGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGgcgag < 2:101562/251‑1 (MQ=255) tcttATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATa < 1:182251/250‑1 (MQ=255) aCAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAgttgtt > 1:179782/1‑250 (MQ=255) gtgcTCGCTGAAGGTCAGCTCATTGGGTTGATTAGCAACGTTCACAATCTGCTCAAGAACTGCTTTGGTGTACAGGTGAAGGTCCACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTGTTCTTCCTCTTGGTCCAGCTGGCTGCGCACTTCAGGTAACTGGTGCTGTAAGAATTCGAGGTTTTATAAATTCTGCGTCGCCTGGCTAGCTATATTCTGTTGCAAATAGTTGTTAGCGATGCCGTTCAGaa < 2:71761/248‑1 (MQ=255) aTTGAGTTGATTATCAACGTTCACACTCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGCGTAATCAACTGGGGat > 2:290091/1‑251 (MQ=255) gTTGATTATCAACGTTCACAATCTGCTCCAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGACTCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTCACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAACTAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGatcatcc > 2:121393/1‑250 (MQ=255) gTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGatcatc > 1:15901/1‑250 (MQ=255) aaCGTTCACAATATGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAGCTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCCAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCCGTCATgg > 2:25948/1‑251 (MQ=255) tCACAATCTGCTCAAGTACGGCTTTGGCTTCCAGGCTAAGGTCAACAGAATCGCGCTGCTGGCGATAAACGTTGCGTTTTTCTTCCGCTTGGTCAAGCTTGCAGCGAACTTCAGGGAAATGGCGCTGGAAGAATTCAAGGCTTTGTGAATCCTGCGCGGACTGGCGAGCGATATTCTGTTGCGAATAATTGGTAGCGAAGCTGATCAGAATACGCGGAATCAACTGAGGATCATCCACAGTCATGGTAAGt > 2:135378/1‑251 (MQ=255) cTCAAGAACGGCTTTGGCTTCCAGGTTAAGGGCAACCGAATCGCGCGGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCATCTTGCTGCGCACTTCATGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTg < 1:197794/153‑1 (MQ=255) atCAAGAACGGCTTTGGCTTCCAGGTTTAGGTCAACCGAATAGCGCTACTGGCGATACACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGCAACTGGCGCTGTAAGAATTAAAGGCTTTGTGAATCCTGCGCCGCCTg > 2:197794/2‑153 (MQ=255) aaGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGc > 1:246595/1‑251 (MQ=255) gAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCCCTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAACCAACTGGGGATCAGCACCAGTCATGGTAAGTTCCAGCATCCCGctt < 1:25948/251‑1 (MQ=255) ggCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGAAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTATGTTGCAAATAGTTGTTAGCGATGCTGTTCAGCATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGctttctt > 1:252631/1‑251 (MQ=255) ttGGCTTCCATGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTGTCTTCCGCTTGGTCCAGCTTGCTGCTCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTAc < 1:27456/250‑1 (MQ=255) tCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGtt < 1:290091/250‑1 (MQ=255) ggTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCGTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTa < 2:315917/251‑1 (MQ=255) aaGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACgg > 1:48170/1‑251 (MQ=255) aaGGGCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCGTTGTCCAGCTGTCTGCGAACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCTCCGCCTGGCGAGCTATATTCTGTTGCAAATAGGTGTTAGCGATGCTGTTCAGAATACGAGTAATCCACTGGGGATCATCTCCATTCATGGTAAGTTCCAGCATCCCGCTTTCTTTTCTGCGTTTGCTAACgc < 2:178362/251‑2 (MQ=255) cAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTaa > 1:43769/1‑249 (MQ=255) ggCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGCATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTAACGGTTTCCTGCAATGCGtt > 2:111168/1‑250 (MQ=255) aTAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTAAAGGTTTCCTGCAATGCGTTAATCg < 2:91529/251‑1 (MQ=255) ttttCTTCCGCTTGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGCATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTACTCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTAAAGGTTTCCTGCAATGCGTTAATCGCTTCCCGTTCGGt > 2:94815/1‑251 (MQ=255) tGGTCCAGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTAAAGGTTTCCTGCAATGCGTTAATCGCTTCCAGTTCGGTACGCTGGCTc > 1:9313/1‑249 (MQ=255) aGCTTGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCACCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTAAAGGTTTCCTGCAATGCGTTAATCg < 2:90429/220‑1 (MQ=255) | TTATTCAGGCGTTTGCGTTCTTGCTCCAGCGTCTGGCGTTTTTCCAGCAGCGCACGATAAGTTGGGTGATCTTTCTTATACAGCTGGGAGATCTCTGCCTCGCGGAAGGTCAGCTCATTGAGTTGATTATCAACGTTCACAATCTGCTCAAGAACGGCTTTGGCTTCCAGGTTAAGGTCAACCGAATCGCGCTGCTGGCGATAAACGTTGAGTTTTTCTTCCGCTTGGTCCAGCTCGCTGCGCACTTCAGGTAACTGGCGCTGTAAGAATTCAAGGCTTTGTGAATCCTGCGCCGCCTGGCGAGCGATATTCTGTTGCAAATAGTTGTTAGCGATGCTGTTCAGAATACGAGTAATCAACTGGGGATCATCACCAGTCATGGTAAGTTCCAGCATCCCGCTTTCTTTACTGCGTTCGCTAACGGTAAAGGTTTCCTGCAATGCGTTAATCGCTTCCAGTTCGGTACGCTGGCTC > NC_000913/1043132‑1043605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |