Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,001,251	A→C	Q679P (CAA→CCA)	elfC →	putative fimbrial usher protein ElfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,001,251	0	A	C	100.0%	30.5 / NA	12	Q679P (CAA→CCA)	elfC	putative fimbrial usher protein ElfC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/5);  total (7/5)

CAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGAT  >  NC_000913/1001012‑1001486                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            cAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTCTCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGAGCCAGCCTGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCACCACCCACTGCACTAcc                                                                                                                                                                                                                                  >  2:271667/1‑250 (MQ=255)                                     cAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGc                                                                                                                                                                                               >  1:117455/1‑250 (MQ=255)                                            gATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGCACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTAcccc                                                                                                                                                                                       >  2:247833/1‑250 (MQ=255)                                            gATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCt                                                                                                                                                                                       >  1:231141/1‑251 (MQ=255)                                                                    aaCCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGCGTGGGCTCCAACTACAGCGATAACGGCAGTCCACAACCACTGAACTACGCTCTTTCAGGCAGCTTAGTTGCCCATTCACCGGGCATTACCCTGGGGCACTCGCTGGGGAAAACTa                                                                                                                                                                >  2:304276/1‑250 (MQ=255)                                                                                                                         gCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCAcc                                                                                                           <  2:117455/250‑1 (MQ=255)                                                                                                                                                              ggTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCCGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCGGGCGCAAAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGCTATACCGTTGTTcc                                                                     >  2:106575/1‑251 (MQ=255)                                                                                                                                                                              aTTACAAAGGGCCGTTTGCTGCTGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCATTGAACTGCGCTCTGTCAGCCAGTTTAGTTGCCCATTCACAGGGCAGTACCCTGGGGCAATCGCTGGGGGAAACGATCGTTCTGATTGCAGCACCATGCTCAGAGAATACTCGTGTGGCGAACATCTCCGGGCTGAAATCTGACTGGCGCGGATATACCGTTGTTCCTTATGCCCCTTCTTAt                                                     <  2:79467/251‑1 (MQ=255)                                                                                                                                                                                                     gCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTAGCGGGAAAATCGAATCGCACTTg                               <  1:117421/250‑1 (MQ=255)                                                                                                                                                                                                           gTGGGCTACAACTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATgcggc                        >  1:54207/1‑251 (MQ=255)                                                                                                                                                                                                                     aCTACAGCGATAACGGCAGTCAACAACCACTGAACTACGCTCTTTCAGGCAGTGTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGTCGAACAGCACCGGGCTGAAAACTGACTGGCGCGTATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCTTCGTTaaaa              <  2:253250/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGGCAGGCAACAACCACTGAACTACGCGCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGAt  <  1:1449/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            CAGGAAAAAAAATTACGCCTGGATGACCTCTAACACCAGTATCGATAACGAAGGGCACACTACACAAAACCTGGGTTTAACGGAGACATTACTCGATGACGGTAACCTGAGCTACAGCGTGCAACAGGGATATAACAGCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGAT  >  NC_000913/1001012‑1001486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑