Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	951,274	A→G	*761Q (TAA→CAA)	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	951,274	0	A	G	100.0%	83.1 / NA	23	*761Q (TAA→CAA)	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

AGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTG  >  NC_000913/951053‑951503                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      aGGAGTGATTGCGTACAATAACTGTCATTTTGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTTTTGGTGCGCAGCTCTAAGGCTACGGCTATTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCCCGCTAAAAAAGGCCCCACTTTCTTGGAGTCTTTATTGTACGCTTTTTACTTTACGATTTCAGTCAAATCTAAGTGCATATATTCAGTGAAGGTACGAGtaataa                                                                                                                                                                                                          <  2:271580/251‑1 (MQ=255)           gccaCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTGTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCctgctg                                                                                                                                                                                                <  2:56273/248‑1 (MQ=255)                  aTAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTTCGCAGCTCGAAGGCTACGGCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCGTGTTACTGTTCTGTTTCAGCCAAATCTAAGTGCATAGATTGAGTGAAGGTACAAGTAATAACGTCCTGCGTCTGTTCt                                                                                                                                                                                         <  2:143399/251‑1 (MQ=255)                              gCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGACGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCg                                                                                                                                                                               >  1:134867/1‑249 (MQ=255)                                                          gAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGTCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGCCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                                                                                                                                    <  2:216228/248‑1 (MQ=255)                                                                    tGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCa                                                                                                                                        <  1:77664/250‑1 (MQ=255)                                                                     ggTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGc                                                                                                                                      >  1:282045/1‑251 (MQ=255)                                                                                cGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTTCTGTACGATTTCAGTCAATTCTGATTGCATAGATTGAGTGAAGGTATGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCGGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                                                                                                                           <  1:311075/251‑1 (MQ=255)                                                                                        aCGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCggg                                                                                                                    <  2:233856/250‑1 (MQ=255)                                                                                        aCGTCGAGTCTGTTTTGGCAGGTAACTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCGTTAGTCAGCGAGTTGAAACGTACTGCGGAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCggg                                                                                                                    <  2:211416/250‑1 (MQ=255)                                                                                                      ttGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGCGTAATCCCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg                                                                                                     >  2:87477/1‑251 (MQ=255)                                                                                                                         atagatagCTGACAAAAAAGGCTCTCGCGCTAACAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtc                                                                                   >  1:200439/1‑250 (MQ=255)                                                                                                                         atagatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTGATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGGACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtc                                                                                   <  1:146939/250‑1 (MQ=255)                                                                                                                         atagatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAAGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtc                                                                                   >  1:131291/1‑250 (MQ=255)                                                                                                                                          aaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAgg                                                                 <  1:205009/251‑1 (MQ=255)                                                                                                                                              ctctCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGCTTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGCGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                                                             >  2:311571/1‑251 (MQ=255)                                                                                                                                                                          gTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGGTCAGTTGCTGACCACCTTCGATGGATGCTTCgtggtg                                 <  1:312824/251‑1 (MQ=255)                                                                                                                                                                                       tGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatc                     <  1:87477/250‑1 (MQ=255)                                                                                                                                                                                         tACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat                  >  1:48263/1‑251 (MQ=255)                                                                                                                                                                                                  tACTGTACGATTTCAGTCAAATCTAATTGCATAGAGTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag          <  2:239265/250‑1 (MQ=255)                                                                                                                                                                                                         cGATTTCAGTCAAATCTACTTGCATAGATTGAGTGAAGGTCCGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtttt                                                                                                                >  2:189036/1‑141 (MQ=255)                                                                                                                                                                                                         cGATTTCAGTCAAATCTAATTGCATAGATTGAGTGCAGGAAAGCGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTCCTGCGCAGCAAGATACACGGCTGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGACGTAACCATCCATCAGACCAGCCAGGTTg  >  1:42236/1‑251 (MQ=255)                                                                                                                                                                                                         cGATTTCAGTCAAATCTAATTGCATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtttt                                                                                                                <  1:189036/141‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      AGGAGTGAATGCGACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTG  >  NC_000913/951053‑951503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑