Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,692	T→G	T350P (ACC→CCC)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,692	0	T	G	96.9%	99.8 / NA	32	T350P (ACC→CCC)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (17/14);  minor base A (1/0);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTA  >  NC_000913/822453‑822927                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            tGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCCGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACct                                                                                                                                                                                                                                  >  2:308582/1‑251 (MQ=255)    ggATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGATCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACctgct                                                                                                                                                                                                                               >  1:296157/1‑251 (MQ=255)    ggATCCTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTGCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTTTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTTGTCAGGTTCCGCCATGTGCGTTTGGGCAGCATGGGTTCATCCACctgct                                                                                                                                                                                                                               <  1:46726/251‑1 (MQ=255)     gATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACctgctg                                                                                                                                                                                                                              >  1:159609/1‑251 (MQ=255)            tttGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAA‑GTGGAACGCCAGCACGCTTTTTGTCAGGTTCCAACAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTg                                                                                                                                                                                                                      >  1:275432/1‑251 (MQ=255)             ttGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATc                                                                                                                                                                                                                        <  1:107399/249‑1 (MQ=255)                   ggcgcCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACACCCGTGTTTTCAGATGCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGGGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGAGGCGTACAAAGTTGAAAGCCAACAAGGTCTTGCGCAGGTTCCACCAGGTGTGGGTGTGCCGCATGAGTTCATCCACctgattcacattttcggca                                                                                                                                                                                                                 >  2:18126/2‑234 (MQ=255)                        cagcgggtgtgtTATTTCCTCATCAGGGTTTTACCCCCGTGTTTTCAGTGTCTACCCGATCCTGCGTTGCCATTGTCGGTTGTGCGGGCTGATCAACCTGCGCCATACTTGGCGTGGGGGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTTGCGTAAAAAGTTGTACGCCAGCGCGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGTAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATg                                                                                                                                                                                                           <  2:172542/243‑1 (MQ=255)                        cAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCACCCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCCGGTGCGTTTGGGCAGCATGGGTTCATCCCCCTGCTGACCATCTGCGGCAATAATg                                                                                                                                                                                                           >  2:243768/1‑251 (MQ=255)                                     aTTTACTCATCAGGGTTTTACACCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCCTTGTCGGTTGAGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGGAGCCAGCCAACCCGCGCCGGGAAGTGGCGGAAAAAGTGGAACGCCAGCACGCGTTTGGACAGGTTACACCAGGGGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTAt                                                                                                                                                                                              >  1:292385/1‑251 (MQ=255)                                             atcaGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCCTTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGc                                                                                                                                                                                      >  1:200101/1‑251 (MQ=255)                                                 gggTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCt                                                                                                                                                                                  <  1:308582/251‑1 (MQ=255)                                                            ccGTGTTTTACGTTTCTACCCGATCCTGCGTTTCCATTGTCGGCTGTGCGGTCGGTTCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCCGCGCCGGGCAGGGGCGGAAAAAGTGGAACGCCCGCCCGCTTTTGGTCAGGTTACACCAGGAGCGGTTGGGCAGCATGGAGTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGGTCCGCTTATCGCGCAGCGTCTGGTTAAAATgg                                                                                                                                                                       >  2:281851/1‑250 (MQ=255)                                                                             aCCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGatgatg                                                                                                                                                        <  1:243768/249‑1 (MQ=255)                                                                                                     ggTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATg                                                                                                                                                                                                           <  2:89793/174‑1 (MQ=255)                                                                                                     ggTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATg                                                                                                                                                                                                           >  1:89793/1‑174 (MQ=255)                                                                                                      gTTGTGCGGGCGGATCAACCTGCGCCAGACGTTGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCAGGCTTTTGGTCAGGTTCCATCAGGTGCGTTTGGGCAGCATGGGTTCATCCTCCTGCTGACAAGCTGCGGCAAGAATGCCGGTCATATTATCGCGCGGCGGCTGGTTATAATGTCGATCGTGGATGATGCCATTTTCTTCGAGTTTCAGGGACaaa                                                                                                                             <  2:192818/251‑1 (MQ=255)                                                                                                           gcgggcggATCAACCTGCGCCACACGTGGCGTGCGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGCGGCGTAAAAAGTGGTACGACAGCACGCTTTTGGTCAGGTTCCACCAGGCGCGTTTGGGCAGCATGGGATAATCCACCTGCTGACAATCTGCGGCAATAATGACGTTCAGATTATCGCGTAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGACTCGGGATTCAGTGACAAACTg                                                                                                                          >  1:208258/1‑249 (MQ=255)                                                                                                                          ggcgcCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACt                                                                                                         <  1:264500/250‑1 (MQ=255)                                                                                                                                   cGTGGCGTGTGTGCCGGAAACCAGCCAACCAGCGCCGGGAATTGGCGTAAAAAGTGGAACACCATCACGCTTTTGGTCAGGGTCCACCAGGTGCGTTTGGGCTGCATGGGTTCATCCACCTGCTGACAATCTTTGGCAATAATGCCGTGCAGAGTAGCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGAGGACGTTTGCTTCGATATTCAGTTACAAACTGAGCGGATCGAGATTAGTGGACCCTAc                                                                                                <  2:47614/251‑1 (MQ=255)                                                                                                                                         gtgtgtGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGc                                                                                          >  1:237115/1‑251 (MQ=255)                                                                                                                                                        cagccaACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCAt                                                                           >  2:169971/1‑251 (MQ=255)                                                                                                                                                                                    aaaaGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAgcgggcgg                                               >  2:248944/1‑251 (MQ=255)                                                                                                                                                                                         tGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGggcggcg                                             >  1:127290/1‑248 (MQ=255)                                                                                                                                                                                         tGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCGACGGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGggcggcg                                             <  2:127290/248‑1 (MQ=255)                                                                                                                                                                                           gAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGATCATCCACCTGCTGACAATCTGCGGAACTAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGCCGATCGTGGATGATGACATTTGCTTCGAGATTAAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGcggcggcggg                                        >  1:273843/1‑250 (MQ=255)                                                                                                                                                                                             aCGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCAGGGGGTCAGCCGCCTGCTGACAAGCTGCGTCAATAATGTCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATGGTGGATGAGGACATTTGCTTCGAGATTCATTGTCAAACTGATCTGATCTAGATGACTGGGCGCTACTGCCGCTTAGTGATCGTCCATCAATGGCACTTTGTGGTGGAGCCGGCGTCGGCGGTAc                                      <  2:25844/251‑1 (MQ=255)                                                                                                                                                                                                 cAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCCCTTTGCCGTGGAGCGGGCGGCGGCGGTACTCa                                   >  2:148997/1‑250 (MQ=255)                                                                                                                                                                                                            tgatCAGGTACCACCAGGTTCGTTTGGTCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAACGCCGTTCATATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATTTCATCTGCTTCCAGATTCAGTGACAAACTTAGCGGAGCGTGATGCCTGTGCCTGACCTTCGCCCAGTGATCGTCCATCAATGCCTCTCTGCCGTGGAGCTGGCGGCGGTGGTTCTCAAAAACCTGa                          <  2:144054/245‑1 (MQ=255)                                                                                                                                                                                                              gTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAACCTGAAcgcc                     <  1:169971/251‑1 (MQ=255)                                                                                                                                                                                                                           aGGTGCGTTTGGGCAGCATGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGCGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCCAAAACCTGAACGCCGCCTTTAAcccgg        >  2:286071/1‑248 (MQ=255)                                                                                                                                                                                                                                 gtgtGGGCAGCCTGGGTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCTGTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGAGCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGCGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCTGCGGCGGTACTCAAAAACCTGAACGCCGCCGTTAACCAGATAGTta  <  2:203802/248‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            TGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGATGATGACATTTGCTTCGAGATTCAGTGACAAACTGAGCGGATCGAGATTACTGGACCCTACTGTCGCCCAGTGATCGTCCATCAATGCCACTTTGCCGTGGAGCGGGCGGCGGCGGTACTCAAAAACCTGAACGCCGCCTTTAACCAGATAGTTA  >  NC_000913/822453‑822927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑