Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,452	G→T	A6E (GCA→GAA)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,452	0	G	T	94.1%	43.3 / ‑5.5	17	A6E (GCA→GAA)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (6/10);  minor base C (1/0);  total (7/10)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

AACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAAC  >  NC_000913/754207‑754679                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     aaCAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCttt                                                                                                                                                                                                                                  <  1:80178/250‑1 (MQ=255)    aaaCTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTa                                                                                                                                                                                                                              <  1:34004/251‑1 (MQ=255)           cAGGTCGTTAGAATCGGACGCAAGCTGGTAGATCGGGAAACCGCGGAGCAGCAAAATACCTTCATCAGCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTAGAAGTGAAGCCAGGGTCAAAGGAGAAAACACCTTTTGAACCGAGAGTACGGATATCCATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTGTCTTTTGTCTCAGcc                                                                                                                                                                                                                        >  2:207387/1‑250 (MQ=255)              ggaggtAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATGTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCGTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑ttt                                                                                                                                                                                                                   <  2:77375/246‑1 (MQ=255)                     gAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGtct                                                                                                                                                                                                            >  1:307616/1‑251 (MQ=255)                           gTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAg                                                                                                                                                                                                      <  1:14048/251‑1 (MQ=255)                                         gatcgGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTa                                                                                                                                                                                        <  1:73979/251‑1 (MQ=255)                                                       ggTGCAGCAAAATACCTTCATCACAATCAATAAAAGTAATTTTAGATTCACAGGATGCGGTTGAAGTGCAGCCTGGGACACAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGATGTATCCACGTTGAGGGTGCGTTTTTATTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACt                                                                                                                                                                          >  2:156988/1‑251 (MQ=255)                                                              caAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                                                                                                                                                   <  1:58280/251‑1 (MQ=255)                                                                         catcaCCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGTGTGAGTTTTTCTTTTGTATCATCCA‑TTTAAGTTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcaca                                                                                                                                                        <  1:292500/251‑1 (MQ=255)                                                                          atcaCCCTCAATAAGAGTAATTTTCGTTTCGCAGGAAGAGGTTGAAGTGAAGCATGGGTCACAGGTGAACACACCTTTTGAACAGAGAGTACGGATATCAATAACGTCTTGACCCACCGTGCCTTTCAGAACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTACGGTCTCCTTAGCGACTTATTGTGTAAGACTGCCGGAACGAAAATTTGCCTTCGCACAt                                                                                                                                                       >  2:215273/1‑251 (MQ=255)                                                                                                  aGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTa                                                                                                                               <  2:307616/251‑1 (MQ=255)                                                                                                                         tgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaa                                                                                                          >  1:6515/1‑249 (MQ=255)                                                                                                                                          gttaaCACACTTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCGCCTTAGCGCCTTATTGCGTAAGACTGCCGGATCTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTa                                                                                       <  2:13439/248‑1 (MQ=255)                                                                                                                                                  acCTTTTGAACCGCGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGctc                                                                               >  1:61455/1‑251 (MQ=255)                                                                                                                                                                                                                         cAGCTGTATCCCCGTTGAGGGTGAGTTTTTCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAatta        <  2:92392/251‑1 (MQ=255)                                                                                                                                                                                                                               tATCCCCGTTGACGGCGCGTTCTCCTTCTGTATCA‑CCACTTTAAGCGCTCCCTCGAGCCTTATTGCGTACGTCTGCCGGTACTTAAATTTACCTTCGCACATCAACCTGGCTTTACCAGTTTTTTATTTGGCTCGCCGCCCTGTGACAGAGGGGAAAACCCGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGATTAAATGACGAATAACGTACTTAGCAACCCGTATTATTAAAc  >  2:21008/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     AACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCA‑TTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAAACCTGGGTACAGAGCTCTGGGCGCTTGCAGGTAAAGGATCCATTGATGACGAATAAATGGCGAATCAAGTACTTAGCAATCCGAATTATTAAAC  >  NC_000913/754207‑754679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑