Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,614,343 G→A intergenic (+83/+337) yjjV → / ← yjjW putative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,3430GA94.4% 37.9 / NA 19intergenic (+83/+337)yjjV/yjjWputative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (11/6);  minor base C (1/0);  total (13/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTA‑CAGGT‑TTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATA  >  NC_000913/4614111‑4614470
                                                                                                                                                                                                                                         |                                                                                                                                
atGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGt                                                                                                                 >  1:76310/1‑249 (MQ=255)
  gCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGTCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTGTAACGTGCCGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCAGTTGGCGCACAATGCCGGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAgg                                                                                                              <  1:118731/250‑1 (MQ=37)
   ccGCTCAACGGTTTTCAGGGGAAGCCTACCCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGCTGCACCGGGAACCGGCGGATGAGAATGCGCAACCGTTGCTTAATAACCCGTATACGTTGTTTAACGTGCCGTAGGACGGATAACGCGTTCACGCCGCCTCCGGCCGTAGGCGCACAATGCCTGATGCGACACTTGTCGAGGCTTATCATGACTAACAG‑C‑CTGTGCCGAACCGTAGGcc                                                                                                            >  2:267451/1‑251 (MQ=255)
    cGCTCACCGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGCTGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTTCCGTAAGCCGGATAAGGCGATCACGCAGCATCCGGCCGTTCGCGCACAATGCCTGATGCGACGCTTGTCGCATCTTATCATGCCTA‑CACGTCTTGT‑CCGAACCGTaacccc                                                                                                           >  2:272141/1‑246 (MQ=255)
                              aaCCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGGCTTATCATGCCTA‑CAAGT‑CTGTGCCGAAACGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                 >  2:3025/1‑251 (MQ=25)
                                    ccGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGCGTTTCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGAAAAGGCGTTCACACCGCATCCGGCAGTTGGCGCCCCATGCCTGATGCGACGCTTGTCGCTTCTTATCATGCCTA‑CAAGT‑CTGTGCCGCACCGTAGGCCGGCTAAGGCATTccacacacatccgtcagccgg                                                                           >  2:271614/1‑231 (MQ=255)
                                    ccGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTTTCGCGTCTTATCATGCCTA‑TAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                     <  1:303237/241‑1 (MQ=255)
                                    ccGGAGCAGGCTACCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTACTAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTTGGACGGAACAGGCGTTCACGCCGCATc                                                                                     >  2:303237/1‑241 (MQ=21)
                                        ggCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTGGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATACGTTGTTTAACGGGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGca                                                                       <  1:3025/250‑1 (MQ=18)
                                           gggCTGCCGGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGcataa                                                                    <  1:85209/250‑4 (MQ=18)
                                              cTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGc                                                                 >  1:86459/1‑251 (MQ=18)
                                                              cGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGAAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGATTGTCGAGTCTTATAATGCCTA‑CAAGT‑CTGTGCCGAAACGAAGGCAGGATAAGGCGTTCACGCCGCATCCCGAAGTCGGCGCATAATGCCTGATGCGACGCTTGt                                                 >  1:186872/1‑251 (MQ=17)
                                                               gTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTc                                                >  1:240063/1‑251 (MQ=255)
                                                                tGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcg                                               >  1:41747/1‑251 (MQ=255)
                                                                 gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcg                                               <  1:5606/250‑1 (MQ=255)
                                                                                       ccGGCGGATTAGATTGCGCACGCGTTGCTTAATAACTCGTAGACGTTGTTGAACGTGCCGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTAGCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACaggttt                        <  1:286458/251‑6 (MQ=11)
                                                                                              aTGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCg                  >  1:178013/1‑250 (MQ=11)
                                                                                                      gcgcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg         >  1:287865/1‑251 (MQ=11)
                                                                                                               ttGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGCACCGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATa  >  1:277762/1‑249 (MQ=11)
                                                                                                                                                                                                                                         |                                                                                                                                
ATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTA‑CAGGT‑TTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATA  >  NC_000913/4614111‑4614470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑