| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,599,224 | A→G | V73A (GTC→GCC) | opgB ← | phosphoglycerol transferase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,599,224 | 0 | A | G | 96.3% | 82.7 / ‑6.4 | 27 | V73A (GTC→GCC) | opgB | phosphoglycerol transferase I |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (16/10); minor base T (1/0); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TTATAATAAGCCGCAAAGTCCGGGTCGCCGTCGCGTGACTGGGATTTCACCAGTTCCGTTATCTGACGAAATGCCGGGCTGGCGTCCACCGAACCCAGCGCCAGTAAGAGCGCCAGCAGGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCG > NC_000913/4598990‑4599467 | ttATAATAAGCCGCAAAGTCCGGGTCGCCGTCGCGTGACTGGGATTTCACCAGTTCCGTTATCTGACGAAATGCCGGGCTGGCGTCCACCGAACCCAGCGCCAGTAAGAGCGCCAGCAGGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTc < 1:218022/249‑1 (MQ=255) gCCGGGCTGGCGTCCACCGAACCCAGCGCCAGTAAGAGCTCCAGCAGGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGGCAGCCCCAGCACAATGCCTATACCCGGCAAAAGGGAGTTGCTTGCGCCAGCACCTGTCAGGCGGGTGGTTAAGGTATAGAGATCCGCGGCGTGAATACCATCGCCAGTAAAAGAGTCGCTGGcttatagggt < 2:158609/251‑10 (MQ=255) gggCTGGCGTCCACCGAACCCAGCGCCAGTAAGAGCGCCAGCAGGCTGTACCCAAAATGGTGCGGATGATGGAGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAAAGTATTTGCTGGCGCCAGCCCCGGTCAGGCTGTTGGGTAAGGTATAGAGAACCGCGTCGTTAATAACCTAGCCAGTAAAATAGTCGCttacaaaccaggaga > 2:244871/1‑236 (MQ=255) ccAGCGCCAGTAAGAGCGCCAGAAGGCTGTAACCAAAATGGCGCGGATGCTGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGATAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCCGTAAAATAGTCGCTGGCAAACAGGGAGATATTTAAAACGACAACTAGc > 1:216789/1‑251 (MQ=255) cAGTAAGAGCGCCAGCAGGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGACCACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAACATGTATTTGCTGTCGCCCGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTCAAATAGTCGATTGCAAACAGGGTGATATTTAAACCGACAAATAGCCCCatc > 2:119010/1‑248 (MQ=255) aGTAAGAGCGCCAGCAGGCTGTAACCAAAAGGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGGATGTGCTGGCGCCAGCACCGGTCAGGCTGTTGTTTAAGGTATATAGAACCGCTTCGTTAATACCATCGCCAGTAAAATAGTCTCTGGCaaa < 1:34845/215‑1 (MQ=255) gTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCAACGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCGGCAAacc > 1:105119/1‑251 (MQ=255) ccAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAaccacc > 1:274287/1‑250 (MQ=255) gTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTAc > 2:68518/1‑251 (MQ=255) cGGATGATGGAGACGACGGCGCAGGATCCAGCACAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGACGATACCCAGCAAAATGTATTAGCTGGCGCCAGCACCGGTCAGGCTGATGGTTAAGGTATAGAGAACCACGTCGTTAATACCATCGCCAGTAAAATCGACGATGGCAAACAGGGTGACATTTAAAACGACCAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTc > 2:36374/1‑251 (MQ=255) ggATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAAcc < 2:62679/138‑1 (MQ=255) ggATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAAcc > 1:62679/1‑138 (MQ=255) gacgacGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCAt < 1:249222/251‑1 (MQ=255) cgacgGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGc > 1:171702/1‑251 (MQ=255) gcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGCGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaa > 1:181965/1‑250 (MQ=255) ccatcacaCTGCCGATTCCCGGCAAAATGTTTTTGCTGGCGCCAGCACCGTTCAGGCTTTTGGTAAAGGTATAGAGACCAGCGTCGTTAATACCATCGCCATGAAAATAGGCTCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGCCCCGCTTTCCAGGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGATAGTAGTTCt < 2:7975/247‑1 (MQ=255) gCCGATACCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGATGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCAAGTAAAATAGGCGCTGGCAAACAGGGTGATCTTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGAGTTCCGTACCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGAAGTTCTGACAACTGGc > 1:54808/1‑251 (MQ=255) aCCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATcc < 2:274287/250‑1 (MQ=255) aCCCGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCGTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCt < 1:115955/251‑1 (MQ=255) ccGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAg > 2:226807/1‑196 (MQ=255) ccGGCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAg < 1:226807/196‑1 (MQ=255) gCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATCTTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCAt > 1:308336/1‑182 (MQ=255) gCAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGGTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAATCGACAAATAGCCCCAGCACCGTTAACGGGGCTGCAAATCACCAGGTGTTACGTCCCGTTTTCCAt < 2:308336/182‑1 (MQ=255) cAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAACAGGGCGAAAGAGCGTAGTTCTGACAACGGGCGATCCTCACTa > 2:9862/1‑250 (MQ=255) cAAAATGTATTTGCTGGCGCCAGCACCGGTCAGGCTGATGGATAGGGTCTAGAGAACCGCGTCGTTCATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGAGATATTTAAAACGACAACTAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGCAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTa > 1:299380/1‑250 (MQ=255) aTGTATTTGCTGGCGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCCTCGCCAGTAAAATAGTCGATGGCAAACAGGGAGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCAACAGGAGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAACCCg > 1:270210/1‑251 (MQ=255) tttGCTGGCGCCAGCACCGGTCCGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCg > 2:27486/1‑251 (MQ=255) | TTATAATAAGCCGCAAAGTCCGGGTCGCCGTCGCGTGACTGGGATTTCACCAGTTCCGTTATCTGACGAAATGCCGGGCTGGCGTCCACCGAACCCAGCGCCAGTAAGAGCGCCAGCAGGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCG > NC_000913/4598990‑4599467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |