Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	657,294	G→A	M1M (ATG→ATA) †	cspE →	transcription antiterminator and regulator of RNA stability CspE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,294	0	G	A	100.0%	30.3 / NA	13	M1M (ATG→ATA) †	cspE	transcription antiterminator and regulator of RNA stability CspE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/4);  total (9/4)

TTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTG  >  NC_000913/657131‑657481                                                                                                                                                                    |                                                                                                                                                                                            ttAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTc                                                                                                      >  1:41217/1‑251 (MQ=255)               aaaaTCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGc                                                                                        >  1:167751/1‑251 (MQ=255)                  aTCAAAAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCGCTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCatt                                                                                     <  2:8924/251‑3 (MQ=255)                               aTAAGTAACATCAAAAATAACGCGCCTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGCAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATATCTAAGATTAAAGGAAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGt                                                                        >  1:191087/1‑251 (MQ=255)                                  aGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCAAAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTCAAGGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGtttt                                                                     >  2:93469/1‑251 (MQ=255)                                                 aaCGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGa                                                       >  1:162903/1‑250 (MQ=255)                                                            tATCACTTCGTAGAAAAGTTATACTGGCCAAAGCGGAACACAATTGGAGCACTGGAAACCAACCCAGAATTGGAGTCTATTTTCCCTGTAAAGGTAATTTAGATATCTAAGATTAGAGGTAACGTTAAGTGGTTTAATGAGTCTAAAGGAGACGGTTGCATTACTCCGGAAGACGGCAGCACAGACGTGTTCGTAAACTTCTCTGCAATCAAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGcgcg                                            >  2:158674/1‑250 (MQ=255)                                                                        gTAAAGTTACACTGGACAAAGCGTCCCACAATTGGAGTACTGGAAAAAGACACAGCATTTGTGTCTATTTTTAATGCAAAGGAAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCAAATGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGACTTCGTACACTTCTCTGCAATCCAGAATAATGGTTTTAAAACTCTTGCTGACGTTCAGCGCGTAGAGTTCGAAAt                               >  2:36985/1‑251 (MQ=255)                                                                                       aCAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCaa                <  1:254392/251‑1 (MQ=255)                                                                                                aCCACAATTGGTGTACTGGTAACCGACACATCAGTTGTGTCGATTTTTCATGTAAAGGTAATTTTGATAGCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACTGCAGCAAAGACGTGGTCGTACACTTCTCTGCAATCCAGACGAATGGTTTTAAAACACTTTCTGAAGGTCAGCGCGTAGAGGTCTAAATCACTAACGGTGCCAAAGGCCCtt        <  2:191087/250‑1 (MQ=255)                                                                                                  cacaATTGGTGTACTGGCAACCGACACAGCATTTGTGTCTATTTTTCATGTAACGGAAATTTTGCTATCTAAGATTAAAGGTAACGTTACGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCAAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAACGGCCCTTctg     >  2:305832/1‑251 (MQ=255)                                                                                                     aaTTGGTTTACGGGTAAACGACACAGGATTTGTGTCTATTTTTCATGTAAATGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGTAGCAAAGACGTGTTAGTACACCTCTCTTCAATCCAGACTAATGGTTTTAAATCTCTTGCTGAAGGTCAGCGCGGAGAGTTCGAAGTCACTATCGGTGCTAAAGGCCCTTcttctg  <  2:276217/251‑1 (MQ=255)                                                                                                     aaTTGGGGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATATCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgctg  >  2:23305/1‑251 (MQ=255)                                                                                                                                                                    |                                                                                                                                                                                            TTAGTAACTTCTTTAAAATCAATAGCTAAAATAAGTAACATCAAAAATAACGCGACTTTTATCACTTTTTAGTAAAGTTACACTGGACAAAGCGTACCACAATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTG  >  NC_000913/657131‑657481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑