Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,454,080	A→G	G166G (GGA→GGG)	yjfF →	galactofuranose ABC transporter putative membrane subunit YjtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,454,080	0	A	G	100.0%	72.5 / NA	25	G166G (GGA→GGG)	yjfF	galactofuranose ABC transporter putative membrane subunit YjtF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/14);  total (11/14)

CGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATG‑GGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAA  >  NC_000913/4453839‑4454314                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            cgccgcTGCTGGCGTTTCCGCTAGAGCTGGAGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCACGGCATTTATCATTACGCTTGCGGGGATGATCTTATTGCGCGGCGTCCGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGAGATGGGGCTGTTGATg                                                                                                                                                                                                                                    >  1:102679/1‑251 (MQ=255)           ggCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCggtg                                                                                                                                                                                                                           <  1:109673/250‑1 (MQ=255)                                           ccTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGCTCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGGGGTTATCGGCATATTCCTAGCGCATCGTAcc                                                                                                                                                                                          >  1:222800/1‑251 (MQ=255)                                           ccTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTAcc                                                                                                                                                                                          >  1:277178/1‑251 (MQ=255)                                               cGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCAGTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGtt                                                                                                                                                                                      <  2:117996/251‑1 (MQ=255)                                                           ggggCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGATTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGTCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGGGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAg                                                                                                                                                                           <  1:256121/250‑1 (MQ=255)                                                             ggCTTCTGATCGACGCCCTGAAGATCCCGGCATTTAGCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATGTATGACACGCTCGCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCGGAGGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGta                                                                                                                                                                        <  2:176529/251‑1 (MQ=255)                                                                    gATCGACGCCCTGAATATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTGTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGcc                                                                                                                                                                  <  2:274112/250‑1 (MQ=255)                                                                                             tttATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTaa                                                                                                                                                                               >  1:194317/1‑212 (MQ=255)                                                                                             tttATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTaa                                                                                                                                                                               <  2:194317/212‑1 (MQ=255)                                                                                              ttATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTGAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAgg                                                                                                                                                                          >  1:107977/1‑216 (MQ=255)                                                                                              ttATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCTCGCATCGTACCCGTTTTGGTAATCAgg                                                                                                                                                                          <  2:107977/216‑1 (MQ=255)                                                                                               tagcATTACGCTTGCGGGGATGTTCTTTTTGCGCTGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCGTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGGTATCGGCATATTCCTCGCGCATCGTACCCGTTTTTGTAATCGTTTATACGCCATTGGCGGCAACGCAACGTCGGCGAAt                                                                                                                                       <  1:313362/247‑1 (MQ=255)                                                                                                      aCGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATAGGCATATTCCTCGCGCATCGCACCCGGTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGAAg‑gg                                                                                                                              >  1:48062/1‑251 (MQ=255)                                                                                                                        tttttGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGTCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGGTGGCGGTGGGGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTAGACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATG‑GGGTTTTCCACTCgcagca                                                                                                             <  1:137265/250‑1 (MQ=255)                                                                                                                          tttGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCGCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATG‑GGGATTTCCACTCgccgccccc                                                                                                          >  2:51931/1‑244 (MQ=255)                                                                                                                                                             cGATTCCGATAAACCATCCCATTTATGCCACGATCTCAAGCCTTGCGTGGAAAATCCCTGCCGGCGGTCG‑CTTACGAGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTTGCGCCTCGTACACGTTTTGGTAATAAGTGATACGACATTGGCGGCAAAGCAACGCCGGCGGATTTGATG‑GGGAGTTCCACTCGCAGCACCACCATTCCCATTTATATGCTCTGCCCCGGACttgc                                                                        >  2:124277/1‑247 (MQ=255)                                                                                                                                                                   cGATAAAACATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATCACTCGCGCATAGTCCCCGTTTTGGTACTCCGGTATACGCCGTTTGAGTCACCCCCACGTCGGCGAATCTG‑TGTGGGGTTTTCACTCGCAGCCCCACTATTCGACTTTATATGCTCTCCACCGGTCTGGCagacatg                                                                 >  2:264253/1‑245 (MQ=255)                                                                                                                                                                     gtAAACCATGCCATTTATGACTCGCTCTCAAGTCGTGTGTGGAAAATCCCTGGCGTCCG‑CGTCTTAAGGTCGATGGGGTTGCTGAGGTTTTCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGTCAACGCAGTGTCGGCGAATCTGATG‑GGGATTGCCACTTGCAGCACCACGATGCGCTGTTATATGCTCTCCACCTGACTggttacgctttc                                                               <  2:302263/250‑11 (MQ=255)                                                                                                                                                                                                          tgTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCTGTGGTGGTTATCGGCATATTCCTCGCGCACCTTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCACAGTCGGCGAATATGATG‑GGGATTCCCACTCGCAGCACCCCTATTCGCCTTTATATGCTCCCCACCGGACTGGCACCGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGata                           <  1:51931/249‑1 (MQ=255)                                                                                                                                                                                                              gAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATG‑GGGATTTCCACTCgcag                                                                                                               >  1:215383/1‑162 (MQ=255)                                                                                                                                                                                                              gAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATG‑GGGATTTCCACTCgcag                                                                                                               <  2:215383/162‑1 (MQ=255)                                                                                                                                                                                                                        ggcggcGGTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATG‑GGGATTTCCACTCGCAGCACCACTATGCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTGCTCGATTTATACCCAGGCCGGATATGCGCTggcgggc              <  1:264253/249‑1 (MQ=255)                                                                                                                                                                                                                            gcggTCG‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGGATACGCCATTGGCGGCAACGCAACGTCTGCGAATCTGATG‑GGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGgt        <  2:277178/251‑1 (MQ=255)                                                                                                                                                                                                                                  g‑CTTAAGTGCGATGGGGCTGCTGATGTTGGCGGTGGAGGTTATAGGCATCTTCCTCGCGCATCGTACACGTTTTGGTAATCAGGAATCCGCCATTAGCGGCAACGCAACCTCCGCGAATCTGATG‑GGGATTTCCAATCGCAGCACCACTATTCGCCTTTATCTGCTCTCCACCGGACTGGCAACGCTGGCGGGGGTTTTCTTCTCGATTTTTAACCAGGCCGGGTATGCGCTgggggggggtggggtgggg  >  1:308677/1‑238 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            CGCCGCTGCTGGCGTTTCCGCTAGTGCTGGTGATGGGCTGTGCCTTCGGCGCATTTATGGGGCTTCTGATCGACGCCCTGAAGATCCCGGCATTTATCATTACGCTTGCGGGGATGTTCTTTTTGCGCGGCGTCAGCTATCTCGTTTCGGAAGAGTCGATTCCGATAAACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCG‑CTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATG‑GGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAA  >  NC_000913/4453839‑4454314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑