Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,449,586	G→A	P23S (CCG→TCG)	ppa ←	inorganic pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,449,586	0	G	A	95.2%	120.1 / NA	42	P23S (CCG→TCG)	ppa	inorganic pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/23);  minor base C (2/0);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGGGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTACA  >  NC_000913/4449342‑4449831                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGAAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGAtctc                                                                                                                                                                                                                                                 >  2:309403/1‑251 (MQ=255)       tCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCCGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGGGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATaac                                                                                                                                                                                                                                           >  2:124714/1‑251 (MQ=255)          ccGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATaacaac                                                                                                                                                                                                                                        <  2:70582/251‑1 (MQ=255)             gCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTTCGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACTGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGAGCTGCGTTAGCCGAGATCTCAATAACAACGTa                                                                                                                                                                                                                                     <  1:284086/251‑1 (MQ=255)                 cgtcgGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATg                                                                                                                                                                                                                                 >  2:202060/1‑251 (MQ=255)                        cATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTGCGTATTGGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCg                                                                                                                                                                                                                          <  1:304397/251‑1 (MQ=255)                              cAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGTATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGAt                                                                                                                                                                                                                    <  1:237318/251‑1 (MQ=255)                               cgAACGCCAACCGGACGGCAACGGATCACAGAACCCGTCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGAGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTAGGATTTGATCGTATCTGCGTTAGCCGCGATCTCAATAACAACGTAGATGTCTTCCGGc                                                                                                                                                                                                                        >  2:73560/2‑246 (MQ=255)                                     ccAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCCGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATACCAACGTAGATGTCTTCCGGCCGATCTTTAcc                                                                                                                                                                                                             >  2:181786/1‑251 (MQ=255)                                          cGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAg                                                                                                                                                                                                        <  1:124714/251‑1 (MQ=255)                                           ggACGGCAACGGATGCCTGAACCCGGCTGCGGCGGGGAAGGAGTTGGGAGCAGTACGTCAAACGGGTCACCGGCCATAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACCTGAAGCGGTCAACGAACAGTGCGCCGCTGTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAGCAACGTAGATGTCTTCCGGGAGAGCTTTAGCCGCAgg                                                                                                                                                                                                       <  2:133499/251‑1 (MQ=255)                                               ggCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACg                                                                                                                                                                                                   >  2:111153/1‑251 (MQ=255)                                                               aCCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGt                                                                                                                                                                                   <  2:109547/251‑1 (MQ=255)                                                                                  ggAGTTGGGACCAGTACGTCAACCGTGTCACCGTCCAGAGACAGGTTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCTCGGTGGACATTAAGCGGTCAACGAACAGTGCGCAGCTCGCTTTGTCGATGTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTTCGGCTGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAatat                                                                                                                                                                <  2:74320/251‑1 (MQ=255)                                                                                   gAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATAtg                                                                                                                                                               <  1:25996/251‑1 (MQ=255)                                                                                                  cGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGGTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTTGCAAACAAGTGcc                                                                                                                                                <  2:297527/251‑1 (MQ=255)                                                                                                     ccaCCGGGTCACCGTCCAGAGACATGGTGTGGTTGATGTAACCTTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGTTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCATGTACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTTGCAAACAAGTGCCGAg                                                                                                                                             <  2:306284/249‑1 (MQ=255)                                                                                                           ggTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGtattat                                                                                                                                       >  1:72944/1‑251 (MQ=255)                                                                                                                    ccAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACt                                                                                                                               <  1:176959/250‑1 (MQ=255)                                                                                                                      agagACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTcgc                                                                                                                            <  2:250998/251‑1 (MQ=255)                                                                                                                         gaCAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGcc                                                                                                                         <  1:66613/251‑1 (MQ=255)                                                                                                                                   ggTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTc                                                                                                               <  1:39694/251‑1 (MQ=255)                                                                                                                                                      gCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCAGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAAGGTCTTCGCTTGTTTTCGTCTTCAt                                                                                             <  1:116213/250‑1 (MQ=255)                                                                                                                                                                   aTCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCAcc                                                                               <  1:111417/251‑1 (MQ=255)                                                                                                                                                                                 gAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGt                                                                 >  1:140478/1‑251 (MQ=255)                                                                                                                                                                                      ggTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATAc                                                             >  2:3913/1‑250 (MQ=255)                                                                                                                                                                                       gTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATAc                                                             >  2:280349/1‑249 (MQ=255)                                                                                                                                                                                        tCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGCTGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGATCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTCTTATAGCAAACTCGCGCCGAATGTCTTCGCTTGTTTTCGACTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATAc                                                             >  2:290606/1‑248 (MQ=255)                                                                                                                                                                                             gAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGGCTGCATTTTCCGTTTTCACCAGTTTTAAGACGGTATACAGAGCAgg                                                     <  1:290606/251‑1 (MQ=255)                                                                                                                                                                                              aaCAGTGCGCCGCTCTCTTTGGCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGAGCCGAGTATTATAGCCAACACGCGCCGAATGTCTTCGCTTGTTCTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAACAgg                                                     >  2:70922/1‑250 (MQ=255)                                                                                                                                                                                               aCAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCCGGa                                                    >  2:62244/1‑250 (MQ=255)                                                                                                                                                                                                       ccgcTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGtatt                                                                                                                                         >  1:4309/1‑157 (MQ=255)                                                                                                                                                                                                       ccgcTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGtatt                                                                                                                                         <  2:4309/157‑1 (MQ=255)                                                                                                                                                                                                       ccgcTCTCTTTGTCGATGTCGTATTTGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGGCTTCCGGCAGATCTGTACCCGCAGGGACGTTGAGTAAGCTCAAGGCAGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTTCTTTGTCACCAGTTTGAAGACGGTATACAGAGCAGGAAAATCCATa                                           <  1:149188/251‑1 (MQ=255)                                                                                                                                                                                                                                tGATCGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTg                   <  1:280349/250‑1 (MQ=255)                                                                                                                                                                                                                                    cGGATCTGCGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGAt               <  1:62244/250‑1 (MQ=255)                                                                                                                                                                                                                                    cGGATCTGCGGTAGCAGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTCCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTCAGACGGTATACAGATCAGGACAATCCATAACCGAAGCCGCATTTTTCTTAGTGAGATg              >  2:88440/1‑251 (MQ=255)                                                                                                                                                                                                                                     ggATCTGCGTTAGCCGAGATCTACATAACAACGTCGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATg              >  2:29460/1‑250 (MQ=255)                                                                                                                                                                                                                                           gCGTTAGCCGAGATCTCAATAACAACGTAGATGTCGTCCGGCAGATCTGTACCCGCAGGGACGTTGAGTAATCTCATGTCTGTTTCTTTTAAAAATATGTGGCAAACAAGTGCAGAGTATTATAGCCAACTCGCGCCGAATGTTGTCTCTTGTTTTCGTCTTCATTATCCTTTTTCACCAGTGTTAAGACAGTATACAGAGCAGGAAAATCCATATCCGTAGCCGCATTTTTCATAGTGAGATGAAAg          <  1:314423/248‑1 (MQ=255)                                                                                                                                                                                                                                            cGTTAGCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGAt      >  1:99130/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGCCGCGATCTCACTAACAACGTCGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAACAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGCTTAc   >  2:180951/1‑250 (MQ=255)                                                                                                                                                                                                                                                 gCCGAGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTACa  >  1:166240/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GCATCTTCACCGGCTTCGTCGGTCATTTTCAGAACGCCAACCGGACGGCAACGGATCACAGAACCCGGCTGCAGCGGGTACGGAGTTGGGACCAGTACGTCAACCGGGTCACCGTCCAGAGACAGGGTGTGGTTGATGTAACCGTAGTTGCATGGATAGAACATCGCGGTGGACATGAAGCGGTCAACGAACAGTGCGCCGCTCTCTTTGTCGATTTCGTATTTGATCGGATCTGCGTTAGCCGGGATCTCAATAACAACGTAGATGTCTTCCGGCAGATCTTTACCCGCAGGGACGTTGAGTAAGCTCATGTCTGTTTCCTTTAAAAATATGTGGCAAACAAGTGCCGAGTATTATAGCCAACTCGCGCCGAATGTCTTCGCTTGTTTTCGTCTTCATTTTCCTTTTTCACCAGTTTTAAGACGGTATACAGAGCAGGAAAATCCATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTACA  >  NC_000913/4449342‑4449831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑