Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,423,337	C→T	S164S (TCC→TCT)	ulaE →	L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,423,337	0	C	T	100.0%	237.6 / NA	74	S164S (TCC→TCT)	ulaE	L‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/42);  total (32/42)

CCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC  >  NC_000913/4423091‑4423580                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cccGCTGGGCAGTTAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATGCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGATAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCa                                                                                                                                                                                                                                                 <  2:182578/251‑1 (MQ=255) cccGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCGGGCGATGGAGATCATGGATTAGCCGTTGATGAGCTCTATCa                                                                                                                                                                                                                                                 <  2:183504/251‑1 (MQ=255)       gggCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAg                                                                                                                                                                                                                                            <  1:277950/250‑1 (MQ=255)                  aTGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATAc                                                                                                                                                                                                                                >  1:72106/1‑251 (MQ=255)                    gACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATAcgc                                                                                                                                                                                                                              >  1:167583/1‑251 (MQ=255)                     aCGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATAcgcg                                                                                                                                                                                                                             <  1:95437/251‑1 (MQ=255)                     aCGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATAcgcg                                                                                                                                                                                                                             <  1:62042/251‑1 (MQ=255)                       gcgGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAACGCGCTGGGATACgcgcc                                                                                                                                                                                                                           >  2:99983/1‑250 (MQ=255)                          gTGCGGGCGGAGGGGCTGGAGATTATGCGGAAAGCTAGCGAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTGTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTa                                                                                                                                                                                                                        <  2:50826/251‑1 (MQ=255)                          gTGCGCGCGCAGGGGCTGGAGATTATGCGTACAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAACAAGGCGCTGGGATACGCGCACt                                                                                                                                                                                                                         >  2:108555/1‑250 (MQ=255)                            gCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTAtc                                                                                                                                                                                                                      <  1:9198/251‑1 (MQ=255)                             cGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGATTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTAtct                                                                                                                                                                                                                     >  1:231415/1‑251 (MQ=255)                                gcgcAGGGGCTGGAGATTATGCGTAAAGCTCTCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTCTGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTAGACGTTTCCGTGACGGCCTGAAAGAGACAGTTGAGATGGAAAGCAGCGAGCAGGGTACACTGGCGATGGAGCTCATGGATTATCCGGTGATGAGATCTAACAGCCAGGAGCTGGGATACGCTCACTATCTca                                                                                                                                                                                                                   >  1:284720/1‑250 (MQ=255)                                       ggcgggAGATTGTGCGTAAAGCTATCCAGTTCGTCCAGGTGGTCGGTATCCGCGTGATCCAGCTTGCGTGCTATGACGTTTACTATCAGGAAGCCAATAACGAGACGCGTCTTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCATGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGGTCTGGGATACGCGCACTATCTcaagtatccg                                                                                                                                                                                                           <  2:208052/247‑8 (MQ=255)                                        gCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACGATCCGt                                                                                                                                                                                                          <  1:91970/251‑1 (MQ=255)                                           ggAGATTCTGCGTAAAGCTATCCAGTTCGCCCAGGCTGTCGGTATTCGCGTGCTCCAGCTGGCGGGCTCTGACGTTTACTATCAGGAAGCCAATAACGCAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGt                                                                                                                                                                                                       >  1:276740/1‑251 (MQ=255)                                           ggAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATCACGAAACGCGTCGTCGTTTCCGTGACGGCCTGACAGAGAGAGTTGAGATGGCCAGCCGCGCGCTGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGCTACGCGCACTATCTCAACAATCCGTGGt                                                                                                                                                                                                       >  2:260571/1‑251 (MQ=255)                                           ggAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTg                                                                                                                                                                                                         <  1:272492/249‑1 (MQ=255)                                             agaTTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATGCGCGCACTATCTCAACAATCCGTGGTTc                                                                                                                                                                                                     <  2:86229/251‑1 (MQ=255)                                                 tATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAg                                                                                                                                                                                                  <  2:307193/250‑1 (MQ=255)                                                  aTGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGct                                                                                                                                                                                                <  1:286782/251‑1 (MQ=255)                                                      gTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTAc                                                                                                                                                                                            >  2:214396/1‑251 (MQ=255)                                                              aTCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGatat                                                                                                                                                                                    >  2:245042/1‑251 (MQ=255)                                                                  aGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGCAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGc                                                                                                                                                                                >  2:36277/1‑251 (MQ=255)                                                                         ccAGGTTGTCGGTAATCGCGTGATCCAGCTGGAGGCCTATAACGTTTACTATCAGGTAGCCAATAACGACACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTCAAGATGGCAAACAGCGAGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCCAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGt                                                                                                                                                                         >  1:268983/1‑251 (MQ=255)                                                                           aGGATGTCGGTATTCGCGTGATCCAGCTGTCGGGCTAGGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTAGCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAAGCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCg                                                                                                                                                                       <  2:268983/251‑1 (MQ=255)                                                                                 tCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTgg                                                                                                                                                                 >  2:312720/1‑251 (MQ=255)                                                                                   ggTATGCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGa                                                                                                                                                               <  1:108555/251‑1 (MQ=255)                                                                                     tATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGAc                                                                                                                                                              >  1:254296/1‑250 (MQ=255)                                                                                          gcgTGATCAGGCTGGCGGGCTATGACGTGGCCTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCGGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCGGGCGATGGAGATCATGGATTATCCGTTGATGAGCGCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGAt                                                                                                                                                        <  2:187495/251‑1 (MQ=255)                                                                                               aTCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGAGCGCAGGTGACGCTGGAGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATACGTGGTTCCAGCTCTACCCGGCTATAGGCAACCTGACGGCGTGGGCAAACGATGTGc                                                                                                                                                    >  2:102815/1‑250 (MQ=255)                                                                                                 ccAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTTGTCGGTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGa                                                                                                                                                 <  2:176686/251‑1 (MQ=255)                                                                                                  cAGCGGGCGGGCTATGACTTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAATCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCTTTGATGAGCTCTATCAGCAAGGCGCTGGGATTCGCGCACTATCTCAACAATCCGTGTTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGTGACAACGATGTGCAGAt                                                                                                                                                <  1:185507/251‑1 (MQ=255)                                                                                                    gcgtgCGGGGTAGGACGTTTCCGATCAGGTAGACAATAACGACACGCGCCTGCGTTTCCGTTACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTATATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCATCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACTACGATGTGCAGAt                                                                                                                                                <  2:162157/245‑1 (MQ=255)                                                                                                     cTGGCGGGCTATGACGTTTACTATCAGGAAGCCACTAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATgg                                                                                                                                              >  1:46255/1‑250 (MQ=255)                                                                                                         cGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTACGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGAATATCCGTTGATGAGCTCTATCAGCAAGGCGCTTGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTg                                                                                                                                         <  1:260571/251‑1 (MQ=255)                                                                                                            gCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGc                                                                                                                                        >  2:198153/1‑249 (MQ=255)                                                                                                            gCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAg                                                                                                                                      <  1:214396/251‑1 (MQ=255)                                                                                                                tGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCATTTCCGTGACGGCCTGAAAGAGAGAGTTGAGATGGCAAGCCGCGCGCAGGAGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGAGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCg                                                                                                                                  >  1:293852/1‑251 (MQ=255)                                                                                                                   cGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGaa                                                                                                                               <  1:36277/251‑1 (MQ=255)                                                                                                                    gTTTACTATCAGGAACCCAATAACGAACCGCCTCGACGTTTCCGTGACCGCCTGAACGAGAGCGCTGCGCTGGCAAGCCGCGCGCAGGTGACGCTGGCGCTGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCCGGCCGGact                                                                                                                              >  2:315876/1‑249 (MQ=255)                                                                                                                       tACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCg                                                                                                                            <  1:280629/250‑1 (MQ=255)                                                                                                                       tACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCg                                                                                                                            <  1:248504/250‑1 (MQ=255)                                                                                                                        aCTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCggg                                                                                                                          <  1:284939/251‑1 (MQ=255)                                                                                                                              aGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATAtc                                                                                                                    <  2:57204/251‑1 (MQ=255)                                                                                                                                  aGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATc                                                                                                                                                                                   <  2:122514/184‑1 (MQ=255)                                                                                                                                  aGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATc                                                                                                                                                                                   >  1:122514/1‑184 (MQ=255)                                                                                                                                   gCCAATAACGAAACTCGTCGTCGTTTCCGTGACGGCCGGAAAGAGAGCGTTGATAGGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCAGGGATTATCCGGTGATGAGCTCTATCAGCAAGTCGCTGGGATACGCGCACTATCTCACCAACCCGTGGTGCCAGCTGTACCCGGAGAGCGGCAGCCTGTCGGCGTGGGAGAGGGATGTGCGGATGAAGTTGAATGCCGGAAGCTGGCATATGGTcgc                                                                                                               <  2:157430/251‑1 (MQ=255)                                                                                                                                   gCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTcgc                                                                                                               <  1:245042/251‑1 (MQ=255)                                                                                                                                           cGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACAtg                                                                                                       >  1:74302/1‑251 (MQ=255)                                                                                                                                            gaagcgcgTCGTCGTGTCGGTGACGGTCGGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGATGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTAGCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAAGCCGGGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACAtgt                                                                                                      <  2:146320/247‑1 (MQ=255)                                                                                                                                                cgcgTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGaaa                                                                                                  <  1:127000/251‑1 (MQ=255)                                                                                                                                                   gtcgtagtTTCCGTGACAGCCTGAAAGAGCGCGTTGATATGGCAAGCCGCGCTCAACAGACGCTGGCGATGGAGATCATGGATTATCCATTGATGAGCTCTATAAGCAAGGCGATGGGATAAGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCAGGATATCGGCAACCTGTCGTCGTGGAACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATCTCGTCGAGGTCCATGGGAAAGa                                                                                                >  2:15015/1‑250 (MQ=255)                                                                                                                                                   gccgtcgtTTCCGTGACGGCATGAAAGAGAACGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGGTACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGGGGGACAACGATGAGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGGGAGAGac                                                                                               >  2:98350/3‑251 (MQ=255)                                                                                                                                                                tgaaggCCTGAAAGAGAGCGTTGAGATGGCAAGCCGAGCGCAGGTGACGCTGGCGAAGGCGATCATGGATTATCCGTTGATGCGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCAAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACCCCTCTGTTCAGATGGAGTTGCAGGCCGGAGTCTGGCATATCGGCGCGGGACATGTGAAAGACACCaaaactagcg                                                                                  >  2:63343/5‑244 (MQ=255)                                                                                                                                                                      ccTGAAAGAGAGCGTTGAGATGGCAAGACGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATACGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGGCCACGATGTGCCGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGCCACCAAACCTGGAGTCTTc                                                                             >  2:175292/1‑250 (MQ=255)                                                                                                                                                                             gagagCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGCCAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGGACATGGGAAAGACAACAAACCTGGCGTCTTCAAAAACGt                                                                     >  2:223181/1‑251 (MQ=255)                                                                                                                                                                                 gCGTTGAGATGGCAAGTCCCGCCCAGGTGACGCTGGCGCTGGAGATACTGGACTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCCCTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATCTCGGCAACCTGCCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGCGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCACAAACGAGCCg                                                                 >  2:34014/1‑251 (MQ=255)                                                                                                                                                                                   gTTGAGATGGCAAGCCGCGCGTAGGTGACGCTGGCGATGGAGAGCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGGACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGtt                                                               <  2:288134/251‑1 (MQ=255)                                                                                                                                                                                      gagaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGGTGGGATACGCGCACTATCTCAACAATCCGTGGGTCCAGCTCTACCCGTATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGGTGCAGGCCGGAATCGGGCATATCGTCGCGTTACATGTGAAATACACCAATCCTTGGGTCTTCAAAAACGTGTCGTTTg                                                             <  2:276740/250‑1 (MQ=255)                                                                                                                                                                                      gagaTGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACTCCAAACCTGGCGTCGTCAAAAACGTGCCGTTTg                                                             <  1:15015/250‑1 (MQ=255)                                                                                                                                                                                                    cgcgcAGGTGACGCTGGCGATGGAGAGCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCGACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTgg                                              <  2:146425/251‑1 (MQ=255)                                                                                                                                                                                                             aaCGCTGGCTATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGTCGCTGGGATACGCGCACTATCTCAACAATCCGGGGTTGCAGCTCTACCCGGATAGCGGCAACCTGGCGGGGTGGGATAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTGCATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAAc                                     <  1:93799/250‑1 (MQ=255)                                                                                                                                                                                                                gCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTATTGGATTTCGAACgtt                                  <  1:223181/251‑1 (MQ=255)                                                                                                                                                                                                                gCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgtt                                  <  1:315876/251‑1 (MQ=255)                                                                                                                                                                                                                gCTGGCGATGGAGATCATGGATTATCCGGTGATGAGCTCTATCAGCAATGTGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTTCCCGGATATCGGCAACCTGTCGGCGTGGGCCAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCATAAACGTGCCGTTTGGCGAAGGTGTATTGGATTTCGAActtc                                  <  2:165542/251‑4 (MQ=255)                                                                                                                                                                                                                   ggCGAGGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCACAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACgttgt                                <  2:303338/250‑1 (MQ=255)                                                                                                                                                                                                                      gATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCg                            >  1:171955/1‑251 (MQ=255)                                                                                                                                                                                                                      gATGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCg                            >  1:128222/1‑251 (MQ=255)                                                                                                                                                                                                                       aTGGAGATCATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCg                            >  1:81031/1‑250 (MQ=255)                                                                                                                                                                                                                               cATGGATTATCCGTTGATGAGCTCTATCAGCAAGGCGATGGAACACGCGCACTATCTCAAAACTCCGTGGTTCCAGCTCTACCCGGATATAGGCAACCTGTCGGCGTGGGACCCCGATGCGCAGATGGAGTTGCAGGCCGGAATCGGGCCTATCGGCGCGGTACATGTGAAGGACAACAAACCTGGCGTCTTCAAAAACGTGCAGTTTGGCGACGGTGGAGTGGATTTCGAACGTTGTTTCGaacagctcc                   >  2:110657/1‑244 (MQ=255)                                                                                                                                                                                                                                      tATCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACaga             <  2:202162/250‑1 (MQ=255)                                                                                                                                                                                                                                       aTCCGTTGATGAGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTACAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGAGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCCTATCGTCGCGGTCCATGGGAAAGACACCAAACCTGGCGTCTTCAAAAACTCGCCGTATGGCGAAGGAGAAGTGGTTTCCGAACGTTGTTTGGAAACGACCAAACagcgg           >  2:237950/1‑248 (MQ=255)                                                                                                                                                                                                                                                 gaGCTCTATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAGCGCTCAAACAGAGTGGCTATTGc  <  1:245655/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGCTATCCAGTTCGCCCAGGATGTCGGTATTCGCGTGATCCAGCTGGCGGGCTATGACGTTTACTATCAGGAAGCCAATAACGAAACGCGTCGTCGTTTCCGTGACGGCCTGAAAGAGAGCGTTGAGATGGCAAGCCGCGCGCAGGTGACGCTGGCGATGGAGATCATGGATTATCCGTTGATGAGCTCCATCAGCAAGGCGCTGGGATACGCGCACTATCTCAACAATCCGTGGTTCCAGCTCTACCCGGATATCGGCAACCTGTCGGCGTGGGACAACGATGTGCAGATGGAGTTGCAGGCCGGAATCGGGCATATCGTCGCGGTACATGTGAAAGACACCAAACCTGGCGTCTTCAAAAACGTGCCGTTTGGCGAAGGTGTAGTGGATTTCGAACGTTGTTTCGAAACGCTCAAACAGAGTGGCTATTGC  >  NC_000913/4423091‑4423580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑