Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	655,829	T→C	H47R (CAC→CGC)	dcuC ←	anaerobic C4‑dicarboxylate transporter DcuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	655,829	0	T	C	100.0%	56.1 / NA	18	H47R (CAC→CGC)	dcuC	anaerobic C4‑dicarboxylate transporter DcuC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/11);  total (7/11)

ACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAAATAATAAAAAATTCCTAAAGCTTAAGGAAAAAATATGCCCAATAAATTGGCGATGA  >  NC_000913/655585‑656054                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  aCAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCa                                                                                                                                                                                                                             <  1:34924/251‑1 (MQ=255)                               cagGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCTCCGTGGCGCATTAGTAATATTTTAACGTATTCAACGATTTCCGTGGCGCTGTAGCCTTTTGAAGCTTGGCTGGACGGTAACACTTTTCGCCCCATAATGGCACTGATAATCAGCAATAACAGGc                                                                                                                                                                                               <  1:259148/251‑1 (MQ=255)                                   tAAGGGGAGTTAAAATAATGCAATGGTTTTGAAGCCAAATTGACAAACATATAATTCGAGCCGATATGGGTCATGTAAGCGGCAAATCAAGCCAGCCTCATAATCCTAATGCCGAGGTAGCCGCCGAGGCTCATTAGTAATATTTTAACGTATTCCACGATATACGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTCGCGCCCCTTAATGACACTGATAATCAGCAATCACAgcgcatc                                                                                                                                                                                           >  2:138377/1‑245 (MQ=255)                                                              tttGACGCCAGCTTGACCACCCTATCATTCGCACCGATCTGGGTCATGTACGCGGCAAATCCACACAGCATCATCATCCTCATGCCGCGGACGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAAAGATCTCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCCCTGATAATCAGCACTAACAGGCCACCGACAAATAacacacaatcggacgagcg                                                                                                                                                                >  2:43332/1‑238 (MQ=255)                                                              tttGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCAGGCTGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGTCATTGATAATCAGCAATAACAGGCCACCGACAAATAacaca                                                                                                                                                                              <  1:43332/237‑1 (MQ=255)                                                                ggACGCCGGCTTGACCACCATATCATTCGCGGCGATATGTGTCATGTAAGCGGCAAATCCACACAGCATCAGAAGCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGGGGCTGAATAcc                                                                                                                                                              <  2:155674/250‑1 (MQ=255)                                                                            gACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGt                                                                                                                                                  <  2:153026/251‑1 (MQ=255)                                                                                   atatCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGc                                                                                                                                           >  1:66127/1‑251 (MQ=255)                                                                                            gcgcCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCcac                                                                                                                                  <  1:119439/251‑1 (MQ=255)                                                                                                                             cacaGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCaatgaa                                                                                                 <  1:119230/251‑1 (MQ=255)                                                                                                                                                                     tCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCCGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAAcc                                                                                                                           >  1:51076/1‑185 (MQ=255)                                                                                                                                                                     tCATTAGTAATATTTTAACGTATGCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAAcc                                                                                                                           <  2:51076/185‑1 (MQ=255)                                                                                                                                                                                             cAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAAATAATAAAAAATTCCTAAAGCTTaa                                 <  2:66127/251‑1 (MQ=255)                                                                                                                                                                                              aaCGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAAATAATAAAAAATTCCTAAAGCTTa                                  >  1:22260/1‑249 (MQ=255)                                                                                                                                                                                                               tGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCCGGCCCCAAAGTAAATAATAAAAAATTCCTAAAGCTTAAGGAAAAAATATGc                    >  2:275066/1‑246 (MQ=255)                                                                                                                                                                                                               tGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAAATAATAAAAAATTCCTAAAGCTTAAGGAAAAAATATGc                    <  1:275066/246‑1 (MQ=255)                                                                                                                                                                                                                gTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAAATAATAAAAAATTCCTAACGCTTAAGGAAAAAATATGCCCAAt               >  1:187665/1‑250 (MQ=255)                                                                                                                                                                                                                            aGCCTGGCTGGACGGTAACACTTTGCGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATTATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATTTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAAATAATAAAAAATTCCTAAAGCTTAAGGAAAAAATATGCCCAATAAATTGGCGATGa  <  1:140041/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  ACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTAATATTTTAACGTATTCAACGATATCCGTGGCGCTGTAGCCTGTTGAAGCCTGGCTGGACGGTAACACTTTGTGCCCCATAATGGCACTGATAATCAGCAATAACAGGCCACCGACAAATAACACACCAGTGGCGGAATACCCTTTAATGATGTAGCGAGCTACACCCACAATAACCACAACCCCAATAAGGAGCTCAATGAATGTCAGCATAATTTTTCCTGTCTCCAGGCCCCAAAGTAAATAATAAAAAATTCCTAAAGCTTAAGGAAAAAATATGCCCAATAAATTGGCGATGA  >  NC_000913/655585‑656054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑