Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,725	C→T	intergenic (+67/‑167)	glyY → / → yjeV	tRNA‑Gly/uncharacterized protein YjeV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,725	0	C	T	100.0%	67.0 / NA	19	intergenic (+67/‑167)	glyY/yjeV	tRNA‑Gly/uncharacterized protein YjeV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/5);  total (14/5)

GTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATC  >  NC_000913/4392490‑4392958                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAAc                                                                                                                                                                                                                             >  1:186289/1‑250 (MQ=255)                                                                tGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTAt                                                                                                                                                             >  1:46551/1‑251 (MQ=255)                                                                    agtgcttaaaggcacagaacgaacAAGCGGGAATAGCTCAGTTGGGAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAAGATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACGCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTAttttt                                                                                                                                                         <  2:253556/228‑1 (MQ=37)                                                                           gaaGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCGCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTAATGCTGGTTTTTGTTGTATCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGCATAGTATTTTTTTAActt                                                                                                                                                  >  2:175167/2‑249 (MQ=255)                                                                                         cccAAGCGGGAATAGCTCAGTTGGTAGAGCAAGCCCTCGCCAAGCACGGGGTCGCGCGTTCGAGTCTCCCTCCCCGCTCCACATTCTTCTCTCAATAAACTATCCACCACGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGt                                                                                                                                    >  1:180863/1‑251 (MQ=255)                                                                                                        cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAACTCTTCTCTCAATAAACTATCCACAGCGACGCGATGCGTTATTGATGGATTTTGTTGTCTCTGATAAACTCTTGTAAACAGCGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTc                                                                                                                     >  1:142784/1‑251 (MQ=255)                                                                                                                       aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTgtcgagtcg                                                                                                      >  2:279999/1‑251 (MQ=255)                                                                                                                             ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAAc                                                                                                                                                    <  1:158161/199‑1 (MQ=255)                                                                                                                             ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAAc                                                                                                                                                    >  2:158161/1‑199 (MQ=255)                                                                                                                                        ggggtggcgatcaagcaactcGTTTCCCGATCAAACATCTTTTCTAAATGACATATCCACAGCGACGAGATCCCTTATTCCCGGTTTTTCTTCTCACTGATAAACTCTTGTAAACAGCGTTATACACCGCCCCAGACTGTAATCTTAATTTCAACGAAACTTCGCACGGTGAATAGTATTTTTTTAACATATTGATAGATAAGTTAAACATTAAGCATCCGTTTTGTCGAGTCGATCACTTGCCGAttat                                                                                      >  2:19168/19‑248 (MQ=255)                                                                                                                                                     cGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAAtt                                                                        >  1:209568/1‑251 (MQ=255)                                                                                                                                                              tttCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACCGCGCAGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGt                                                               >  1:202205/1‑251 (MQ=255)                                                                                                                                                                                tctctcAATAAAATATCCACAGCGACGCTATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTcaca                                              <  2:65626/250‑1 (MQ=255)                                                                                                                                                                                  tctcAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTCTTTTTATTCACAAGc                                           >  1:156689/1‑251 (MQ=255)                                                                                                                                                                                               tCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTTACCTATTgat                                                                                                                                            <  2:224917/141‑1 (MQ=255)                                                                                                                                                                                               tCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTTACCTATTgat                                                                                                                                            >  1:224917/1‑141 (MQ=255)                                                                                                                                                                                                ccACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGGCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAg                             <  1:39964/251‑1 (MQ=255)                                                                                                                                                                                                                       gCTGGTTTTTGTTGTCTCTGATAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTCATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATCGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGCTGAATCAGGCGTCACGCGGTAACCCTTTTTc      >  2:306884/1‑251 (MQ=255)                                                                                                                                                                                                                           gTTTTTGTTGTCTCTGATAAAATCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGCATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATc  >  2:20131/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          GTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAATTCTTCTCTCAATAAAATATCCACAGCGACGCGATGCGTTATTGCTGGTTTTTGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATC  >  NC_000913/4392490‑4392958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑