Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,325,644	C→T	S33N (AGC→AAC)	yjdN ←	PF06983 family protein YjdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,325,644	0	C	T	100.0%	108.0 / NA	38	S33N (AGC→AAC)	yjdN	PF06983 family protein YjdN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/22);  total (16/22)

GATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAG  >  NC_000913/4325406‑4325888                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     gATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATaga                                                                                                                                                                                                                                          <  1:44256/251‑1 (MQ=255) gATTTTTCCGCTAGCGGCCAGATTGTCAAACCAGAGTTTTCCTTTTTCGACATGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGCTGTCGCTTCCGGCATTGCGCACATTGGCGAGGGCGATGGCGGTATCGGGAAATTGAATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTtat                                                                                                                                                                                                                                             >  2:187437/1‑248 (MQ=255)                           cAAACCAGCGTTTTCCTTCTTCGATCTGTTGCGAATCGAGCACCAGAGTAAAGCCGGAGTAGCTGGCTTGTCCTGACGGCATGGCATCGCTCAGCATGATGGCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAAGTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTTCGCTGATTTTGGCACTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCTCCCAACTTACGGTGa                                                                                                                                                                                                                <  2:186860/251‑1 (MQ=255)                            aaaCCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCTGCGTAAAGCAGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGTAATGCGCACATTGGCGTGGGCGAGGGTGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTGCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCTCATCGTACGTTGat                                                                                                                                                                                                               <  2:273068/251‑1 (MQ=255)                                            tcttcGACCTGTTGCGAATCGAGCCCCAGCGTAAAGCCGTAGTTGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCTGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGTGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTGGATTTTATAGAGCAGTTCCGCGCCCAACGTTCGTTGATTATAGGCAATCGCGt                                                                                                                                                                                                <  2:251159/250‑1 (MQ=255)                                            gcttcGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTGTTCCTGACGGCATGGCATCGCACATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATGGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGAGTc                                                                                                                                                                                               <  1:168410/250‑1 (MQ=255)                                                          cGAATCGAGCACCAGCGCAAAGACGGAGTAGCTGGCTTTTCCTGACGGCCTGGCAACGATCAACATGATGTAGATTCCGGCCATGCGCACATTGGCGTGGGCGATGGCGGTATCAGGAAATTGCATTCCGGAAGGGCAGTTTACGGCGCTGTCCTGCGCTGATTTTGGCATTTGGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGTACAGTTACCgg                                                                                                                                                                                 >  2:48030/1‑251 (MQ=255)                                                           gAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGc                                                                                                                                                                                >  1:22624/1‑251 (MQ=255)                                                                  gCACCAGCGTAAAGCCGGAGTAGCTGGCTGTTCCTGACGGGATGGCGTCGCTCATCAGGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAAGTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCGTGCGCTGATTTGGGCATTGCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCATCGGGCGTTGATAATAGGCAATCGCGTCGGAACAGTTACCTGCAAAagag                                                                                                                                                                         <  1:146928/251‑1 (MQ=255)                                                                                ccGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTa                                                                                                                                                            >  1:195741/1‑250 (MQ=255)                                                                                        gCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTGCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCatga                                                                                                                                                   <  1:152020/251‑1 (MQ=255)                                                                                             cTTTTCCTGACGGCATGGCATCGCTCATCATGCTGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCCTTTCGCCGAAGTTGATTTTCTAGAGCAGCTCCGCGCCCAACGACCGTTGATAATAGGCAATCGCGTCGGCACAGTTACCGGAAAAAGAGTGGTAGGGACTTAACGGCATGATGGTg                                                                                                                                              >  2:26316/1‑251 (MQ=255)                                                                                                       cGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTc                                                                                                                                                                                               <  2:127884/192‑1 (MQ=255)                                                                                                       cGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTc                                                                                                                                                                                               >  1:127884/1‑192 (MQ=255)                                                                                                        ggcatggcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCCAAAGAGAGGTAGGGACTTAACGGCATGATGGGGACCTCAggta                                                                                                                                    >  2:179409/1‑247 (MQ=255)                                                                                                         gcatggcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAag                                                                                                                                  <  1:313263/251‑1 (MQ=255)                                                                                                             ggcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTGTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGTCCTCAGTTAAgaga                                                                                                                               <  2:192929/250‑1 (MQ=255)                                                                                                                                       cGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGCAATTGCCTTCCGGACGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCACCGTACATTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTACCGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAAttt                                                                                                     >  2:180155/1‑250 (MQ=255)                                                                                                                                            aTGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGCTTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCAAGATAAGTGTCGTTCGGAATTtataa                                                                                                 >  2:199057/1‑248 (MQ=255)                                                                                                                                               cgcACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATg                                                                                            >  1:20517/1‑251 (MQ=255)                                                                                                                                                   cagtGGCGTGGGCGTTGGCGGTATCGGGAAATTGCATTCCGGGAGGGCAGTTCTCGGTGCTGTCCTGCTCTGATTTTGGCATTTCGCCGAAGTTGATTGTATAGATCAGTTCGGCGCCCAACGTGCGTTGATAATAGGCAAGCGGGTCGGAACAGTTGCCGGGAGAGGAGAGGTAGGGACGGAAGTGTGTGATTGTGACCGCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGACATTTATACCGATGagag                                                                                        <  2:251837/248‑1 (MQ=255)                                                                                                                                                          gTGGGCGCTGGCGGTATCGGGACATTGCATTCCGGCAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGCAGGGACTTAACGGCATGATGGTGACCTCCGTTAAGAGAAGCCAGTTAAGTGGAGTTCGAAATTTATACAGAAGAGAggcgggct                                                                                 >  2:37659/1‑248 (MQ=255)                                                                                                                                                          gTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGtt                                                                                                                       >  1:147372/1‑213 (MQ=255)                                                                                                                                                          gTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCt                                                                                 <  1:179409/251‑1 (MQ=255)                                                                                                                                                          gTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGAGTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGGGACCTCAGTTAAGAGAAGCCAGtt                                                                                                                       <  2:147372/213‑1 (MQ=255)                                                                                                                                                                                    gCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATc                                                         >  2:142081/1‑249 (MQ=255)                                                                                                                                                                                        tCCGGAAGGGCAGTTTTCGGCGCTGTCCTGCGCTTATTTTGGCATTTCGCCTAAGTTGATTTTATAGAGCAGTTCCGAGCCCAACGAACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAATATAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGGTAAGTGTATTTCGAAATTTATACAGATGAGATGCGGCCTGATAAGACGCGCGAGCGGCGCAGCAGGCa                                                    <  2:183900/250‑1 (MQ=255)                                                                                                                                                                                        tCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCa                                                    <  1:199057/250‑1 (MQ=255)                                                                                                                                                                                          cGGAAGGGCCGTTCTCGGCGCTGTCTTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGCTAATACGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTACGAGAAGCCAGTTCAGCGTAGTTCGACATTTCTACAGATGAGACGCGGCCCGATAAGACGCGCGAGCGCGGCATCAGGccatc                                                 >  2:205452/1‑247 (MQ=255)                                                                                                                                                                                              aGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACATTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTGATACAGATTAGAGGCTGCCGGAGAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCa                                             <  1:180155/251‑1 (MQ=255)                                                                                                                                                                                                ggCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCAGCAGGCAGTCGGCACt                                           <  2:283670/251‑1 (MQ=255)                                                                                                                                                                                                      tctcTGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCATTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGc                                      <  1:150623/250‑1 (MQ=255)                                                                                                                                                                                                                gTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATgcggc                            <  2:20517/250‑1 (MQ=255)                                                                                                                                                                                                                   cTGCGCTGATTTTGGCATTTCGCCGAAGTTGCTTTTCTAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTa                         >  1:35422/1‑250 (MQ=255)                                                                                                                                                                                                                          gATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCCGTCGTCACTGTTGCCGGATGCGGCGTAAACGCCtt                 >  2:82565/1‑251 (MQ=255)                                                                                                                                                                                                                          gATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCt                  <  1:22529/250‑1 (MQ=255)                                                                                                                                                                                                                           agtttGGCATTTCGCCGAAGTGGAGTTTAGAGAGCAGTTCCGCGCCCAACTTACGTTGATAATAGTCATTCTCGTCGGAGCAGTGACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATTGTGACCTCAGTTAATAGAAGCCAGTTAAGTTTAGTTCGAAAGTTATACAGATGACAGGCTGCCTGATAAGTCGCGCGAGCGTCGCATCAGGCAGGCGGCACTGTTGCCGTATGCGGCGTAACCGCCTTa                <  2:131163/249‑1 (MQ=255)                                                                                                                                                                                                                                         ccGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg  <  1:1674/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     GATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAG  >  NC_000913/4325406‑4325888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑