Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,262,436	T→C	G256G (GGT→GGC)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,262,436	0	T	C	100.0%	151.2 / NA	45	G256G (GGT→GGC)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/28);  total (17/28)

CGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGCCCATAAAGCGGGTGCAGACATTAATGTGCTGGAACACGCGCTC  >  NC_000913/4262193‑4262682                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        cGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTgg                                                                                                                                                                                                                                                  >  1:27753/1‑251 (MQ=255)  ggCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTggc                                                                                                                                                                                                                                                 >  1:196234/1‑251 (MQ=255)   gCAAAGGCGAGTGTGAGATTTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTggcg                                                                                                                                                                                                                                                <  1:204131/251‑1 (MQ=255)            aGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGAc                                                                                                                                                                                                                                       <  1:275033/251‑1 (MQ=255)                      gTTCATCATCCACGCACTTAAAGCCTGGTTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAAGCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGGGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCt                                                                                                                                                                                                                             <  2:298727/251‑1 (MQ=255)                                        tAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACAGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCg                                                                                                                                                                                                           >  1:174431/1‑251 (MQ=255)                                                             aaGCCCGAAAGAAAAACGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGt                                                                                                                                                                                        >  2:119540/1‑249 (MQ=255)                                                                cccGAAAGAAAACCGGGAAACCCCGCCGCCCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCAGTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTgc                                                                                                                                                                                   <  1:160866/251‑1 (MQ=255)                                                                       aaaaaCCGTGAACGCCCGCTGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCAt                                                                                                                                                                            <  2:70932/250‑1 (MQ=255)                                                                        aaacccttGAATTCCCGCCGCTCGATTATCCGCGTTTGTATCAACTGAAGTGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTGCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATg                                                                                                                                                                           <  2:271569/244‑1 (MQ=255)                                                                         aaaCCTTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGt                                                                                                                                                                          <  1:68418/251‑1 (MQ=255)                                                                              gTGAAATCCCGCCGCTCGATTATCCGCGTGTGTAGCAACTGAAGCGTGACTTTCCGCGTTTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACTCCTGCAACATATGGATGGCGTGAGGGTCGGGCGCGGGGCGTATCAGAATCCTGGCATTCTGGCTGCGGTAGTCCGGGAGATCTTTGGTTGCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCg                                                                                                                                                                     <  2:151093/251‑1 (MQ=255)                                                                                  aaTCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGAGCGCCATGTATCCGTAc                                                                                                                                                                  >  2:210868/1‑250 (MQ=255)                                                                                         ccgcTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCg                                                                                                                                                          >  1:239473/1‑251 (MQ=255)                                                                                         ccgcTCGATTATCCGCGGGTGTTCCAACTGAAGCGTTACTTTCTGCATCTGAAAATGTCGATTAACGGTGGTATCAAGTCGCTGGAATAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCg                                                                                                                                                          <  2:169096/251‑1 (MQ=255)                                                                                          cgcTCGATTATCCGCGTGGGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGt                                                                                                                                                         <  2:27753/251‑1 (MQ=255)                                                                                              cGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGAGGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTGTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGaa                                                                                                                                                      <  2:33755/250‑1 (MQ=255)                                                                                                 ttATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTc                                                                                                                                                   >  1:54771/1‑250 (MQ=255)                                                                                                        cgtttgtATCAACTGAAGCGTGACTTTCCTCATCTGACAATGTCTATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGATGCGTATCAGAATCCGGGCCTTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATATCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAggg                                                                                                                                           <  1:272086/247‑1 (MQ=255)                                                                                                             gtATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGAGTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCgg                                                                                                                                                                                                                 <  1:140747/176‑1 (MQ=255)                                                                                                                   aCTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCAGGTGGCGGTAGTGCGCGCCATGTATCCGTCCATTGAGCGTGAACTCAGCCAGGGGACGTATCTCg                                                                                                                                >  2:274912/1‑251 (MQ=255)                                                                                                                      gAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGcc                                                                                                                             >  1:102599/1‑251 (MQ=255)                                                                                                                             gACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGGTACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTAc                                                                                                                      >  1:75640/1‑251 (MQ=255)                                                                                                                                   ccGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCTCTGGAAGAGGCCAAAGCACACCTTCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTTGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGc                                                                                                                 <  1:192068/250‑1 (MQ=255)                                                                                                                                      cATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAAAGGCCAAAGCACACCTACAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGGAGTGCGCCCCATGTAGCCGGACCTTGAGCGTGGACTAAGCCAGGGGACTGATCTCGGCCATATTACCCGGCatat                                                                                                             >  2:237403/1‑251 (MQ=255)                                                                                                                                        tCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGCCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGAGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGCCCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACCTTGAGCGTGAACTCCGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGt                                                                                                           >  1:316293/1‑251 (MQ=255)                                                                                                                                             cAATGTCGATTAACGGTGGTATCAAGTCGCTGGCAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGCCCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGc                                                                                                      >  1:297693/1‑251 (MQ=255)                                                                                                                                                            gtggtATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCATGGTAtt                                                                                      <  1:119540/251‑1 (MQ=255)                                                                                                                                                            gtggtATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGGGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCTCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTTGGCCATATTTCCCGGCATATGTTGGGCTTG‑TTCCAGGGTAt                                                                                       <  1:289831/250‑1 (MQ=255)                                                                                                                                                            gtggtATCAAGCCGCTGCAAGAGCCCACAGCACACCTACAACATATGGATGGCGAGATGGTCGGGCGCGCGGCGTATCAGAATCCGGGCATTCTGGCGGCGGCAGACCGGGAGATCTTTGGTTCCTCGGCTACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCCCGGCCATATTACCCGGCCTATGTTGGGCTTG‑TTCCAgggcat                                                                                       >  2:289831/1‑247 (MQ=255)                                                                                                                                                                 aTCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTg                                                                                  <  1:134302/250‑1 (MQ=255)                                                                                                                                                                         gCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGACGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑GTCCAGGGTATTCCTGGCGCGCGGc                                                                         <  1:112339/251‑1 (MQ=255)                                                                                                                                                                                 agGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTggcgg                                                                 <  1:236813/251‑1 (MQ=255)                                                                                                                                                                                     cAAAGCACACCTGCAACAGTTGGATGGCGGGATGGTCGGGCGCGAGGGGTATCAGAATCCGGGCAGTCGGGCGGCGGTAGGCCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGGAGCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGtt                                                             <  2:304121/251‑1 (MQ=255)                                                                                                                                                                                          cacaCCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTAACAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCAGGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTa                                                        <  1:306529/251‑1 (MQ=255)                                                                                                                                                                                             acCTGCAACAGATGGATGGCGTGATGGGGGGGCGCGAGGCGGTTCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTGGGGTCCTCGGATACCGATTCCGATCCGGTGGCGGTAGTGCGCGCCATGTAACCGTACATGGAGCGTGAACTCAGCCAGGGGACGGATCTCTGCCATATTACCCGGCAGATGT‑GGGTTTGGTTTCAGGGTATTCCTGGCGCGCGTCAGTGGCTGCGTGATTTAAGt                                                     <  2:144859/251‑1 (MQ=255)                                                                                                                                                                                                  cAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGaaa                                                 >  1:160418/1‑250 (MQ=255)                                                                                                                                                                                                     cATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGc                                             <  1:210868/251‑1 (MQ=255)                                                                                                                                                                                                        atggatggCGTGATGGTCGGGCGCGAGGCGTATCATAATCCGGGCATGCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTGGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTGATTTAAGTTAAAATGccc                                           <  2:297693/250‑1 (MQ=255)                                                                                                                                                                                                          ggatggCGTGATGGTCGGGCGCGAGGCGTATCCGAATCCGGGCATTATGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGaaaaagaccat                                         >  1:101201/1‑243 (MQ=255)                                                                                                                                                                                                                          gggCCCGAGGCGTATCAGAATCCTGGCATTCTGGCGGCCGTAGACCGGGGGATCTTTGGTTCGGCCGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCTTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCGAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGCCCATAAAGCGGGTGCAGACAt                        <  1:69261/251‑1 (MQ=255)                                                                                                                                                                                                                            gcgcGAGGCGTATCAGAATCCGGGCATGCTGGCGGCGGTAGACCGGTAGAGCTTTGGTTCCGCGGATACCTATGCCGAGCCTGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCTTGATCTCAGCCAGGGGACGGATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGCCCATAAAGCGGGTGCATACATTa                      <  1:3182/251‑1 (MQ=255)                                                                                                                                                                                                                                    cGTATCAGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGCCCATAAAGCGGGTGCAGACATTAATGTGCTg              <  1:287537/251‑1 (MQ=255)                                                                                                                                                                                                                                          aGAATCCGGGCATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGGTTTTTAAGTGGAAATGCCCCTAAAGCGGGTGCAGACATTAATGTGCTGGAacac        >  2:139907/1‑251 (MQ=255)                                                                                                                                                                                                                                                cGGGCATTCTGGCGGCGGTAGACCGGGAGATTTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGCCCATAAAGCGGGTGCAGACATTAATGTGCTGGAACACGCGCTc  <  2:239473/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                        CGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCCGGTGGCGGTAGTGCGCGCCATGTATCCGTACATTGAGCGTGAACTCAGCCAGGGGACGTATCTCGGCCATATTACCCGGCATATGTTGGGCTTG‑TTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGCCCATAAAGCGGGTGCAGACATTAATGTGCTGGAACACGCGCTC  >  NC_000913/4262193‑4262682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑