| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,193,996 | T→G | D70A (GAT→GCT) | thiE ← | thiamine phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,193,996 | 0 | T | G | 95.5% | 204.1 / NA | 66 | D70A (GAT→GCT) | thiE | thiamine phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base G (28/35); total (28/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTC > NC_000913/4193751‑4194241 | gCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTg < 1:150693/251‑1 (MQ=255) ccGTGCCATCTGGTCCAGCCCCTGCGGTGCAGAAGGCGTCTGTTTGTTTTGCGTCGGGACCCCATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCTCAGCCGCAGTCTTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGCCGCCATACGCCTGATGCTTGAGCGCCAGCCGCCAGTAAGCGTTGa < 1:255832/251‑1 (MQ=255) aggggtcccAGCCCCGGCGGTGAGGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATAGAAGAGGGGCGGGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACaa < 2:276994/244‑1 (MQ=255) aGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGCTTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCATGATCGCCCGCCGCCAGTACGCGTTGATAAACa > 2:92719/1‑250 (MQ=255) tttCCAGCCCCTTCGGTGCATAAGGAGTCGTTTTGGTTTGCGGCGGGAACCCATGTCCCAGCGAGATACAAGCGGGGCGGGCTGCCGCCGCACCTTCGTTTTCCAGATCGTC‑ATGTGGCGAAACGCCCAGCAGCAGGCCTGCCGCGCGGATGGCATTGAGCGCGGTGGCTTGCAAATCTTCCTGCCCAAAAGGGACGCCATACGCCTGATGCTGGATCGCCAGCCGCCAGTAATCGTTGATAAACAATcg < 2:295531/249‑1 (MQ=255) ccAGCCCCTGCGGTGCAGAAGGCTTCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATcgcgcg < 2:85733/251‑1 (MQ=255) agccccctcGGTGCAGAAGGCTTCGGTTTGGGTTGCGTCGGGAACAAATGCCCAAGGCCGATAGAAGAGGGGCGTGCTGCCGGCGCGGCGTCGATTTCCATATCGTC‑ATGGTTCGAACCGCCCAGCCGCAGGCCTGCCGCGCGGATGTCATTGAGATCGGTGGCTGGAAGATCTTCCTGCCCCAAATGGACGTCATACGCCGGATGCTTGATCGCCGGCGGCCAGTAATCGTTGATAAACAATCGCGCGtt < 2:47160/243‑1 (MQ=255) cGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATAACGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcggc > 1:132067/1‑251 (MQ=255) gTGCAGAAGGCATTTGGGTGGTTTGCGGCGGGAAAACAGGTCACAGCGCGGTATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATGGGTGGCTTGCAAATCTTCCGGCCCCAAATGGACGCCATACGCCTGATGCTTGATGGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGcggcgg < 1:12704/251‑1 (MQ=255) aaGGCATCTTTTTGGTTGGCGTCGGGAACACATGTCCCAGCGCTATATAAGAGGGTCGGTCCGCCAGCGCGCCGTTGATTCCCATAGCGTC‑ATGTGTCGAACCGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATGCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTGGATAAACAATCGCGCGTTATAgcggcgttccagc < 2:130207/251‑8 (MQ=255) aTCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAAt > 1:66818/1‑251 (MQ=255) tGTTTGGTTTGCGGCGGGAACACATGTCCCAGCGCGATAAAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGAGGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTg < 2:22463/250‑1 (MQ=255) gtttggtttgCGTCGGGACCACATGCCCCAGCGCTATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATGTCTTC‑ATGTGTCTAAACGCCCAGCCGCAGGCCTGCCGCGGGGATGGCATTTAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCTTACGCCTGATGCTTGATCGCCAGCCGCCATTAATCGTTGATAAACAACCGCGCGTTATAGCGGCGGCCCAGCGCAATTgc < 1:295365/250‑1 (MQ=255) tttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCCATTgccc > 2:248634/1‑250 (MQ=255) gcgccGGGAACCCATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGACCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACa < 1:241282/247‑1 (MQ=255) gTCGGGCACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCACGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATc > 1:233762/1‑251 (MQ=255) cGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCgg < 1:9027/251‑1 (MQ=255) gAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCCCGACATCGGCtt > 1:219497/1‑251 (MQ=255) cccAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAAGCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTc < 1:113597/251‑1 (MQ=255) cAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCAt > 1:310800/1‑251 (MQ=255) gcgcGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATcg < 1:16777/251‑1 (MQ=255) cgATATAAGAGGGGCGTGCTGCCAGCGCGCCGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCgcgc > 2:182108/1‑251 (MQ=255) taAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCACCCCGCCGATc > 1:257771/1‑251 (MQ=255) gggggCGTGCTGCCATCGCGACGTCGATT‑CCCTAACCTCCAGGTGCCAAAACGCCCGGCCGCAGGCCGGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACTCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTg < 2:220784/250‑1 (MQ=255) gCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGc > 1:264652/1‑251 (MQ=255) gTGCTTCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGACCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGt < 2:163015/251‑1 (MQ=255) gTGCTGCCAGCGCGACGTCGATTTCCCTATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGcc > 2:39296/1‑190 (MQ=255) gTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCGTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGcc < 1:39296/190‑1 (MQ=255) ctgcCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCCGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGc > 1:132649/1‑251 (MQ=255) gcCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTg > 1:8959/1‑251 (MQ=255) gACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgtac > 1:189146/1‑251 (MQ=255) cGTCGATTTCCATCTCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGCGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCCGCGCAATTGCCGCCCCGACCTCGGCTTCCCCCTCTTCATCGCGCCCCTCTTTGATGCGTAGCTGGAGAgtacgt > 2:179117/1‑251 (MQ=255) caTTTCCATAACGTC‑AGGTGTCAAAACGCCCAGCCGCAGGCCTGCCGCTCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAACGGACGCCATACGCCTGATGCTTGATCCCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCTCCACGACATCGTCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGc < 1:302728/250‑1 (MQ=255) ccATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGc > 1:7575/1‑250 (MQ=255) atatCGGC‑ATGTGTCGAAACGCCCAGCCGCAGGCCAGCCGCGCGGATGGCATTGAGATCGGTGGATTGCAAATCTTCCTGCCCCAAAAAGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATc > 1:24701/1‑251 (MQ=255) atatCGAC‑GTGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGCTCGGTGGCTTGCAAATCTTCCTGCCCCAAATTGACGCCATACGCTTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCTCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCGCGACATCGGCGTCCGCCTCGGCATCGCGTCGATCTTTGATGCGTAGCTGGAGAGGACGTACGCCTGCATc < 1:132913/251‑1 (MQ=255) cccgatcaaaaCGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTTCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGAc < 1:34346/243‑1 (MQ=255) ggtggaaccGCCCAGCCGCAGGCCGGCCGCGCGGATGGCATTGAGATCGGTGGCTTACAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTGGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCGTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGtt < 2:146471/243‑1 (MQ=255) gtCGAAACGCCCGGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGAACGCCTGCATCCAACAGACGTTc < 1:110691/251‑1 (MQ=255) cgaacagCCCAGCCGGATGCCTGCCGCGCGGATGGCATTGAGATGGGTGGCTCGCAAGTCTTCCTGCCCCAAAGGGACGCCATACGCCTGAGGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTAGAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTACCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCGGGAGAGTACGTACGCCTGCATCCAACAGAGGTTCg < 2:266068/244‑1 (MQ=255) aaaCGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGGGAGTACGTACGCCTGCCTCCAACAGAc > 2:34346/1‑243 (MQ=255) gccgtcAGGCCCGCCGCGCGGTTGGTTTTGATATCGGGGGCTTGCAATTCTTCCTGCCCAAACTGGACGCCATACTCCTTGTGCTGGAGCGCCAGCCGCCAGTTAGCGTTGATAACCAATCGCGCGTTATATCGGCGGCCCCGCGCAATTGCCGACACGACTTCGGCTTCCACCTCTTCATCGCGCCGATCTTTGTTGCGTAGCTGGAGAGTACGTACGCCTGCATCCATCAGTCGTTCGATCCACTGTAc < 2:244414/246‑1 (MQ=255) gCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTg < 1:182108/251‑1 (MQ=255) gCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACg > 2:154281/1‑244 (MQ=255) gCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACg < 1:154281/244‑1 (MQ=255) ccGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCaccacc < 1:248634/251‑1 (MQ=255) cgcgGATGTCAGTGAGATCGGGGGCTTGCAAATCTCCCCCCCCCAATTGGAACCCATACCCCGGATGCTTGATCGCCAGCCGCCAGTAAGCCGTGAGAAACAACCGCGCGTTATAGCGGCGGCCCAGCGCAATGGCCGCCACGACATCGGCTTCCACCGCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGTCGTTCGATCCACTGTACGCTGTCCACCTCCggg < 2:132067/251‑1 (MQ=255) gcgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGCGAGTACGTACGCCTGCCTCCAACAGACGTTCGCTCCACTGTACGCTGTCCACCACCgggg > 2:57977/1‑250 (MQ=255) cgGATGGCATTGAGATCAGAGGCTTTCAAACCTTCATGCCCCAAATGGAAGCCATACGCCTGATGCTTGCTCGCCAGACGCCAGTAAGCGTTGATAAACAATCGCGCGTTCTAGCGGCGGCCAAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTTATGCGTCGATGGTGAGCACGTACGCCTGAATCCCACACACGTTCGATACCCTTAACGCTGTCCACCACCgggc > 2:45972/1‑249 (MQ=255) aTTGAGCTCGGAGGATTGCCACTCTTTCTGCCCCAAATGGACGCCATACGCCTGATGATTGAACGCCAGCCGCCAGTAAGCGTTGCTAAACAATAGCGAGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATTCGTCGCTGGCGAGTACGTAAGCCTGCATCACACAGACGTTCGATCCACTGTACGCTGTCCAACACAGGGTACCGTCCTg > 2:47997/1‑251 (MQ=255) gaaaTCGGTGGCTTGCAAACCTTCCTCCCCAAAATGGACGCCATACGCCGGATGTTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAAc < 2:189146/248‑1 (MQ=255) gtatgggggctgtgaaaagattCCTGCCCCAAAGGAACCCCTTACGCCGGATGCTTGAGCGCCAGCCGCCAGTAAGCGTTGATAACCAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGGTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACg < 2:52086/231‑1 (MQ=255) aTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGa > 2:296143/1‑250 (MQ=255) tGGCTTGCAAATCTTCCTGACCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCCGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGAAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAATGTACGCTGTCCACCCCCggggagcatcctgcaagaaaaggg > 2:105159/1‑230 (MQ=255) gCAAAGCTTCCTGCCCCAAAGGGACGCAATACGCCTGTTGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCGTCCACCTCTTCACCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGGACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACagga < 2:257771/251‑1 (MQ=255) aTCTTCCTGCCCCAAATGGACGCAATAAGCCTCCTCCTTGATCGCCAGCCGCCAGTAAGCGTTAATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCCTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCCCCGGGGCCAATCCTGAACGAAACGGGACCggagga > 2:176326/1‑250 (MQ=255) tccggCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGGACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGaaa < 1:176326/244‑1 (MQ=255) cGCCATACGCCGTATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACg < 2:132649/251‑1 (MQ=255) aTACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtc < 1:248073/251‑1 (MQ=255) aCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcct > 1:134672/1‑251 (MQ=255) aCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcc < 1:57977/250‑1 (MQ=255) cTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCtt > 1:245591/1‑251 (MQ=255) cGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGAt < 1:42100/251‑1 (MQ=255) cAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAggagga < 1:169591/198‑1 (MQ=255) cAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGAACAggagga > 2:169591/1‑198 (MQ=255) aGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCctc < 1:269299/251‑1 (MQ=255) | GCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTC‑ATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCTC > NC_000913/4193751‑4194241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |