Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,832	C→A	intergenic (‑175/+100)	yijF ← / ← gldA	DUF1287 domain‑containing protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,832	0	C	A	100.0%	72.2 / NA	23	intergenic (‑175/+100)	yijF/gldA	DUF1287 domain‑containing protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/11);  total (12/11)

ATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCG  >  NC_000913/4137618‑4138069                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              aTGCCGTAAGCAGGCTGATCAGCTCCTGTGCAGCTTTACTCTGGTTAGTCCGTGACACTAAGCGTCACAGCTTCGACATTCCAGGATCACGATTGCTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAACTTCCTGACCAACGTGGGCGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCATACAAAAGCACGACAATTCAATATATTGCAGAGATTGCGTAGGCCTGAt                                                                                                                                                                                                           >  2:287493/1‑251 (MQ=255)   gCCGTTAGCAGGCTGAGCAGCGCCAGAGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCTACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGAACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAACAGCACGAAAATTCCATATATTGCAGAGATTGCGTAGGCCTGATa                                                                                                                                                                                                          >  2:235484/1‑250 (MQ=255)      gTTAGCAGGCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCg                                                                                                                                                                                                      <  1:56396/251‑1 (MQ=255)          gCAGGCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTatc                                                                                                                                                                                                  <  2:233243/251‑3 (MQ=255)              gCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCa                                                                                                                                                                                               <  1:223170/250‑1 (MQ=255)              gCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCa                                                                                                                                                                                               >  2:223170/1‑250 (MQ=255)                       cgcCAGTGAAGCTTTCATCTGGTTCGTCCGTGACAATAAGAGACACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAACATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGAGCATCAGGCAAtt                                                                                                                                                                                     >  1:134699/1‑251 (MQ=255)                               aaGCTTTCATCTGGGTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTc                                                                                                                                                                             <  2:239089/251‑1 (MQ=255)                                        tCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCg                                                                                                                                                                    >  1:64313/1‑251 (MQ=255)                                                                         tCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTccc                                                                                                                                    <  1:170154/250‑1 (MQ=255)                                                                          cGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCAc                                                                                                                                  >  2:75419/1‑251 (MQ=255)                                                                                                                     aaGCGGATAAAAAATGCCTGAACAACGTGGGAGTTTAAGATTACTGGCACAGAGTGTCGGATGCGGAGTGATCGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGGAGGCCTGATAAGCGTAGCGCATCAGGCAATTGTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTATGTTATTCTCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTTCCAGCagag                                                                                       <  2:130043/251‑1 (MQ=38)                                                                                                                     aaGCGGATAAAAAAGTCCTGAACAACGTGGGAGTTTCATATTACTGGCTCAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCagag                                                                                       <  2:165454/251‑1 (MQ=255)                                                                                                                             aaaaaaTTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAatatat                                                                                                                                                                                                                                  <  1:282403/104‑1 (MQ=255)                                                                                                                             aaaaaaTTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGAGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTa                                                                                >  1:181009/1‑250 (MQ=255)                                                                                                                             aaaaaaTTCCTGAACAACGTGGGAGTTTCAGATTACTGGCAAAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGACAATTCAatatat                                                                                                                                                                                                                                  >  2:282403/1‑104 (MQ=37)                                                                                                                                             acGTGGGAGTTTCAGATTTCTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTcg                                                               <  2:255230/251‑1 (MQ=255)                                                                                                                                                              tACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgtt                                               <  2:187818/250‑1 (MQ=255)                                                                                                                                                                                     ggAGTGAACGCCTTATCCGACCTACAAAAGCACGAAAATTCAATATAGTGCAGATATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTCAAACTCCCGGTCAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGTAAACGCTGACCGTACTGGTCGGCTGCCAGCAGAGCGGCGTAATCCTGATCTGGCGTCGCGCCTCCAGGCATTTTGGGAATTGGTTCActtgcggcaca                       <  2:197453/251‑11 (MQ=37)                                                                                                                                                                                           aaCGCCTTATACGGCCTACAAAAGAACGAAAATACAATATAATGCAGAGATTGCGTCCGCCTGATAAGCGAAGCGCCTCAGTAAATTTTGCGTTCAAACTACCGGACAAGCCGGGAGTTTGGAGTAGGATATTCCCACTCTTGCAGGAAACGCTGAACGTACTGGTCGGCTACCAGCAGAGCGGCGTACACCTGCTCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGc                 >  2:267984/1‑251 (MQ=255)                                                                                                                                                                                               ccTTATCCGGCCTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGATGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATATGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCt             >  1:113280/1‑251 (MQ=255)                                                                                                                                                                                                cTTATCCGGCCTACAAAAGCACGAAAATTAAATATATTGCAGAGATTGCGTAGGCCTGATACGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGAACATGCCGCTTCTg            >  2:161255/1‑251 (MQ=255)                                                                                                                                                                                                          cTACAAAAGCACGAAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGAAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCg  >  1:15112/1‑251 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              ATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTCCGGGCTAACGATTGTTCTGTAGAGAAAATGTCCAGAAGCGGATAAAAAATTCCTGAACAACGTGGGAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCG  >  NC_000913/4137618‑4138069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑