Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,073,736	A→G	intergenic (‑165/‑3)	yihU ← / → yihV	3‑sulfolactaldehyde reductase/6‑deoxy‑6‑sulfofructose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,073,736	0	A	G	95.8%	84.7 / ‑6.3	24	intergenic (‑165/‑3)	yihU/yihV	3‑sulfolactaldehyde reductase/6‑deoxy‑6‑sulfofructose kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (15/8);  minor base C (1/0);  total (16/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGATGCCCTTGCTGCAATAAATTGCTCGCCATTGGCGAACCCATTTGTCCTAAACCGATAAACGCGATTGCTGCCATAACCCTCTCCTGAATACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGACCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCAGGTCGATTTTATTGGTCGCGTAGGTGATGACGACACGGGCAACAGCCTGCTGGCAGAGCTGGAATCCTGGG  >  NC_000913/4073494‑4073970                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           ttGATGCCCTTGCTGCAATAAATTGCTCGCCATTGGCGAACCCATTTGTCCTAAACCGATAAACGCGATTGCTGCCATAACCCTCTCCTGAATACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGAtt                                                                                                                                                                                                                                    >  1:15175/1‑251 (MQ=255)   gATGCGCTTGCTGCAATAAATTGCTCGCCATTGGCTAACCCATTTGTCCTAAACCGATAAACGCGATTGCTGCCATAACCCTCTCCTTAATACTGTTATGTCACTTTTTGTCATTTATGACATTCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTc                                                                                                                                                                                                                                   <  2:79651/250‑1 (MQ=255)                                                               ccgatcttGCTGCCATAACCCTCTCCTGAATACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCg                                                                                                                                                                        <  2:98392/243‑1 (MQ=255)                                                                   gATTGCTGCCATAACCCTCTCCTGAATACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCAACGgag                                                                                                                                                                  <  1:89509/251‑1 (MQ=255)                                                                      tgctgcCATAACCCTCTCCTGAATACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCg                                                                                                                                                                        >  1:98392/1‑242 (MQ=255)                                                                                   ctctCCTGACTACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCATGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATCGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGCACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACgcgg                                                                                                                                                  >  1:302317/1‑248 (MQ=255)                                                                                    cctcCTGAATACAGTTATGTCTCTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGc                                                                                                                                                 <  2:299981/250‑1 (MQ=255)                                                                                        cTGAATACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCg                                                                                                                                                >  1:65604/1‑248 (MQ=255)                                                                                             tACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGAt                                                                                                                                                                                                                                     >  1:223453/1‑158 (MQ=255)                                                                                             tACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGAt                                                                                                                                                                                                                                     <  2:223453/158‑1 (MQ=255)                                                                                                                                               ttGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCTCTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCa                                                                                       >  1:24654/1‑250 (MQ=255)                                                                                                                                               ttGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCa                                                                                       >  1:88929/1‑250 (MQ=255)                                                                                                                                                     gTATTTGTAATTTATCGTCAAAAAATTGACAGACGTCACTTTTTAACCGATTGGTGAGATTAAAATGAACGCATCCCAAAATGTTTCAGCAAAGCCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTCTTACGAGGCAGGGTTACCGACGGAGAGCGGTATATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCTGCGGCGACGGCAGCGGTTGCGGCGGAAAAGCTgg                                                                                >  1:302446/1‑251 (MQ=255)                                                                                                                                                      tATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTggg                                                                               >  1:149981/1‑251 (MQ=255)                                                                                                                                                                    cGTCAAAAAATGGACCGCCGTAACTTTTTAAACAATTGGTGAAAGTAAAATGAACGCATCTCAAAATGGTTAAGGAATGGCCATGATTCGTGTTGATTGTGTAGGTATAACCGTGCTGGATCGCATCTATTACGGGGAAGGGTTACCGACGGAGAGCGGTAAATTCGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGTGGTTGCGGCGGAAAGGCTGGGGGCGCAGGTCGAtt                                                                 <  2:73973/251‑1 (MQ=255)                                                                                                                                                                             aTTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCAGGTCGATTTTATTGGTc                                                        <  1:205045/251‑1 (MQ=255)                                                                                                                                                                             aTTGACAGCCGTAACTTTTTAAACACTTGGTGAAATTAAAATGAAAGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGGTGGGGGCGCAGGGCGATTTTATTgggc                                                        >  2:309057/1‑249 (MQ=255)                                                                                                                                                                                   aGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCAGGGCGCTTTTATTTGTCGCGtcg                                                  >  1:57692/1‑249 (MQ=255)                                                                                                                                                                                                tAAACAATTGGTGAAATTAAAATGAACGCATCCCACAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCAGGTCGATTTTATTGGTCGGGTAGGTGATGGCGacac                                     >  2:41249/1‑251 (MQ=255)                                                                                                                                                                                                 aaaCAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCAGGTCGATTTTATTGGTCGCGTAGGTGATGACGACACg                                    <  1:75166/251‑1 (MQ=255)                                                                                                                                                                                                         ggTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGGAGGTCGATTTTATTGGTCGCGGAGGGGATGACGAAACGGGCAAc                              >  2:226692/1‑249 (MQ=255)                                                                                                                                                                                                           tGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCAGGTCGATTTTATTGGTCGCGTAGGTGATGACGACACGGGCAACAGcc                          >  1:120103/1‑251 (MQ=255)                                                                                                                                                                                                                       gAACGCCTCCCAAAATGTTTAAGGAATGGCCCTGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCCTCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGCAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGGTGGGGGCGCCGGGCGCTTTTATTGGTCGCGTAGGTGAGGTAGACACGGGCGACGGCCTGCTGGCAGAGc              >  2:14263/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaaTGTTTAAGGAATGGCCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCCGGTCGATTTTATTGGTCGCGTAGGTGTTGACGACACGGGCAACAGCCTGCTGGCAGGGCTGGAATCCTggg  >  1:193479/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           TTGATGCCCTTGCTGCAATAAATTGCTCGCCATTGGCGAACCCATTTGTCCTAAACCGATAAACGCGATTGCTGCCATAACCCTCTCCTGAATACAGTTATGTCACTTTTTGTCATTTATGACATGCTTTGCTTGTCTGTTTTTGATCGTATTTGTAATTTATCGTCAAAAAATTGACAGCCGTCACTTTTTAAACAATTGGTGAAATTAAAATGAACGCATCCCAAAATGTTTAAGGAATGACCATGATTCGTGTTGCTTGTGTAGGTATAACCGTGATGGATCGCATCTATTACGTGGAAGGGTTACCGACGGAGAGCGGTAAATACGTGGCGAGAAATTATACGGAAGTTGGTGGCGGGCCAGCGGCGACGGCAGCGGTTGCGGCGGCAAGGCTGGGGGCGCAGGTCGATTTTATTGGTCGCGTAGGTGATGACGACACGGGCAACAGCCTGCTGGCAGAGCTGGAATCCTGGG  >  NC_000913/4073494‑4073970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑