| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,856,886 | G→A | intergenic (+22/+25) | yidI → / ← yidJ | putative inner membrane protein YidI/putative sulfatase/phosphatase YidJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,856,886 | 0 | G | A | 89.7% | 70.8 / ‑6.0 | 29 | intergenic (+22/+25) | yidI/yidJ | putative inner membrane protein YidI/putative sulfatase/phosphatase YidJ |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/15); minor base C (3/0); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
AAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCGGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCG > NC_000913/3856657‑3857118 | aaGCGGTATCGCACTGGCCATTGCTGCGCTACGTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTa < 1:126603/251‑1 (MQ=255) gccgatcgcgcTACTTTGCGCATTGATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCAGACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGAGATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGttt < 2:186885/244‑1 (MQ=255) ttGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCAcc < 1:263274/249‑1 (MQ=255) ggTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTTACTGGGTTTAGCAGACCGAATAATGCCCGCCAGGGG‑ATCATCCGCGGGCCTTATCGTCAAGACTTTTGTTTTTTTTCCTCCCCCCTCCCCCCCTGTGTCggg > 2:34209/1‑250 (MQ=255) aTGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAgg < 1:192127/251‑1 (MQ=255) aGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGgtgt < 2:86445/251‑1 (MQ=255) aTCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGAGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGGTATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGGTTTAGCAGCCCACATAAATCCCGCCCGGTGGATC‑TCAGCGGGCATTATCGGCCGCACCTCTTTTTTTTTTCCTCCCCCTTCACCCCTTGTTTCGGGAGGGCGGTGTtatact > 2:225161/1‑249 (MQ=255) cATTCGTGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTTAACAGCCTGAATAATGCCCCCCAGGTG‑ATCATCAGCGGGCATTTTCGTCCGAACTTCTGTTTTTTTTCCTCCACCTTCACCCCTTGTGTCGGTTGGCCGGTGTCATAAGCGCGGACAAc > 1:90401/1‑251 (MQ=255) cggcgCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGATTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAACCCGAATAATTCCCGCCAAGGG‑ATCATCAGCGGGCATTTTAGTCCGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGGCATAGTTGCGTACAACTGGCgt > 1:288956/1‑250 (MQ=255) cgcgcTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCGCCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATa < 1:130492/251‑1 (MQ=255) gcggcgGGACGCTGGCTTTCCATACCTTGTTTTTTGGTATCGGCATCGGGGGCAGCATTTTGCTGATATTCCTTATCTTTTCCTGCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTgtggac < 2:119481/251‑1 (MQ=255) gggACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGGTTAAGCCGCCTGCATAATGCCCGCCCGGTG‑ATCATCCGCGGGGATTATCGTACGAACTTCTGTTTTTTCTCCTCCACCTTCTCCCCTTGTGTCGGTAGGCCCGTGTTCTAGTCCCGGACCACTGTCGAATAGCCATCTTgtggtctgggg > 2:2432/1‑244 (MQ=255) gCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGaaa < 1:34209/251‑1 (MQ=255) tGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGGGGACGAAACg > 1:290157/1‑251 (MQ=255) tGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTTTGGACGTGGGCGAAACGCCCCCATCCAGcgcg > 1:206969/1‑251 (MQ=255) aTCGGTATCTTGTGCAGCATTGTGCTGATATTCCTTATCTTTTCCTTCCTTTCCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATGATCGTCATAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGTTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACTTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATc < 2:272146/250‑1 (MQ=255) tCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAAATGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCtt > 1:153854/1‑251 (MQ=255) cATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGGG‑ATCATCAGCGGGCATTATAGGCAGAACTTCTGTTTTTTTTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTAATAGTCGCGTACAACTGGCGAAAAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGGCGTGCATCTTTa > 1:249398/1‑249 (MQ=255) gtgtGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAACAGCCTGAATAATGCCCGCCCGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTATGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGGCGTGGGCGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCAc > 1:280563/1‑251 (MQ=255) gcagcaTTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGAc < 1:198144/251‑1 (MQ=255) gcaTTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCa < 2:220976/251‑1 (MQ=255) ccttcctGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTAGCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGAt < 2:280563/251‑1 (MQ=255) cgtccggACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATTCCCGCCAGGTG‑ATCATCAGCGGTCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGGCTGTAGGCCGGTGTCATAGTCGCGTACACCTGGCGAATAGCCATCGTGTGTACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTTCGCCACGGACGCAGACTCCATGGGTAACCGCGGAACGGATc < 2:316314/245‑1 (MQ=255) tcctGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGTACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATcg < 2:97386/250‑1 (MQ=255) cggcggTTTACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCCGCGGGCCTTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTTCATCTTTACGCCACGGGCGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAAtttt > 2:91505/1‑251 (MQ=255) tCACTGGTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCtcctcc < 1:283272/83‑1 (MQ=255) tCACTGGTTTAAGCACCCTGAATAATGCCCGCCAGGTG‑ATCATCAGAGGGCCTTATCGTCAGAACTTCTGTTTTTTCtcctcc > 2:283272/1‑83 (MQ=255) gTTTAAGCAGCCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATcc > 1:126354/1‑251 (MQ=255) gcCTGAATAATGCCCGCCAGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCg > 2:89175/1‑251 (MQ=255) | AAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGCGCTGGTTTTTGGCGGCGGGACGCTGGCTTTCCATACCTTGCTTTTTGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGCCCGCCGGGTG‑ATCATCAGCGGGCATTATCGTCAGAACTTCTGTTTTTTCTCCTCCACCTTCACCCCTTGTGTCGGTAGGCCGGTGTCATAGTCGCGTACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCG > NC_000913/3856657‑3857118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |