Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,828,830	T→C	intergenic (‑165/‑115)	gltS ← / → xanP	glutamate:sodium symporter/xanthine:H(+) symporter XanP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,830	0	T	C	100.0%	55.1 / NA	18	intergenic (‑165/‑115)	gltS/xanP	glutamate:sodium symporter/xanthine:H(+) symporter XanP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/7);  total (11/7)

GACGGAATGGACCAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTTATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACA‑GCGAAC‑TGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTC  >  NC_000913/3828585‑3829065                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              gaCGGAATGGACCAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTAGCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTACGCAGAAAAATACCAAAAGTcatata                                                                                                                                                                                                                                          <  2:22118/251‑1 (MQ=255)                                            gCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGCTTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGAtgtg                                                                                                                                                                                               >  1:106694/1‑251 (MQ=255)                                               gCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCa                                                                                                                                                                                            <  1:218188/251‑1 (MQ=255)                                                       cGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTg                                                                                                                                                                                    >  2:39463/1‑251 (MQ=255)                                                                 gTATCGAGATGAAACCTAGTTACTACTTTGTATCCACATCACTGTTTCATATCCGTCATACTTCATCGAGCAGTGGCAACTTCTCGAACTATGACAGATATCGAGTTCTGCTTTCCTGGGACAAACTATCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATACAAAAACGCATTTAACTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCtt                                                                                                                                                                          >  1:107271/1‑251 (MQ=255)                                                                                                   cGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAAGTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTGAGATGAGGATGTGGCAAACGTTTGGTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACgg                                                                                                                                        <  1:161249/251‑1 (MQ=255)                                                                                                               tCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTGTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCg                                                                                                                             <  1:107011/250‑1 (MQ=255)                                                                                                                          aTACTTCATCGCGCAGGGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTACCGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATATCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTACTTTTCTCTTTCGGCCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTcc                                                                                                                 >  2:199187/1‑251 (MQ=255)                                                                                                                                   ggggcattgtcAACTTCATTAAATATGACGTGTATCGAGTTATGCTTTTCTGGGTGAAAGTCTCAGCTGTTAATGAACAAATGTCATTGATTTTAAGCAGTAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGCTGAGTATGTGGTAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTGTGCTACCACTGGATTGTTGTCGATTTCTTTGTCCACCCTctag                                                                                                        <  2:213062/241‑4 (MQ=255)                                                                                                                                      gcAGTGGCAACTGCATGAACTATGACAGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGGATGGATTTTAAGCAGAAAAATTCCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTGCGGTCATCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCa                                                                                                     <  2:160084/251‑1 (MQ=255)                                                                                                                                        aGTGGCAACTTCATGAAATATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTc                                                                                                      >  1:87394/1‑248 (MQ=255)                                                                                                                                                                      cGAGTTATGCTTTCATGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGTTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGACAATGCGCAACCGGTTGCGCAGACTCaac                                                                      >  2:186237/1‑249 (MQ=255)                                                                                                                                                                                                    gCTGTTAATGAACAAATGGCATGGATTTTACGCAGACATATACCAAAAGTCATAACAAAAAGTGCATTTATCTCATTGAAATATGTAGCTGAGGATTTCGCAAACGTTTTCTTTTCTCTTTCGGTCAACTAACATGCCCGCTTTTCTACCACGGGATTGTTTTCGCTGTCTGGTTCCACCCTCGTGTCAGAAAATGCGCACCCGTGTGCGCAGAGTCACAACA‑GCGCAT‑CGATTTACAGTCTTGAAGCTCg                                     >  2:170013/1‑251 (MQ=255)                                                                                                                                                                                                       gTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACA‑GCGAAC‑TGATTTACCGTCTTGAAGATCGTcc                                  >  2:233414/1‑251 (MQ=255)                                                                                                                                                                                                        ttAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGTATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACA‑GCGAAC‑TGATTTATCGTCTTGAAGATCGTccg                                 >  2:265972/1‑251 (MQ=255)                                                                                                                                                                                                          aaggAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATTAGGATGTGGCAAACGTTTGCGTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGTAACCGGTTGCGCTGACTCAAAACA‑GCGAAC‑TGAGTTACCGGCTTGAATATCGTccgcc                               <  2:256539/248‑1 (MQ=255)                                                                                                                                                                                                             gAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACA‑GCGAAC‑TGATTTACCGTCTTGAAGATCGTCCGCCGCt                            >  1:185197/1‑251 (MQ=255)                                                                                                                                                                                                                                    cAGAAAAATACCAAAAGTCATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACA‑GCGAAC‑TGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt     <  1:186237/251‑1 (MQ=255)                                                                                                                                                                                                                                       agaaatatctcaaGTCATATAAAAAAGTGCATTTATATAATTTAAATATTTAGATGAGGCTGAGGCAACCGTTTGCTTTTCTCTTTCGGTCAGCTAACATGCCCGCTTTGCTACCACGGGTTTTTTTTCGATGTCTGTTTCCACCCTCGAGTCCGACACTGCGCAACCGGTTGCGCAGACT‑AAAACAAGC‑AACATGTTTTACCGTCATGAAGTTCGTCCGCCGCTTCCTCAAACCCTTTTTGCCGCCtctc  >  2:251618/12‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              GACGGAATGGACCAACTTACGCCCGAGCAGCAACGTCAGCGTTGCGGCAACAAGCGTTGCTAAAGTATCGAGATGAAACATAGTTACTCCTTTGTATCCGCATCACTCTTTCATACTCGTCATACTTCATCGCGCAGTGGCAACTTCATGAACTATGACGGGTATCGAGTTATGCTTTCCTGGGTCAAATTCTCAGCTGTTAATGAACAAATGGCATGGATTTTAAGCAGAAAAATACCAAAAGTTATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACA‑GCGAAC‑TGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTC  >  NC_000913/3828585‑3829065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑