Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,716,923	C→T	A126V (GCG→GTG)	yiaD →	PF13488 family lipoprotein YiaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,716,923	0	C	T	97.3%	118.4 / NA	38	A126V (GCG→GTG)	yiaD	PF13488 family lipoprotein YiaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/21);  minor base G (0/1);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGAAGAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGC  >  NC_000913/3716679‑3717154                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        gTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGAAGAAAGATCGCGGTAAAGGAGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGAGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGAAAGCAGAAGCGCGACACTGAAACCGGTGGgcgc                                                                                                                                                                                                                                   >  2:163266/1‑251 (MQ=255) gTGGGCGCGGGTATTGGTGCGCACTCTTCTTCGAAGAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGgcgc                                                                                                                                                                                                                                   >  1:98846/1‑251 (MQ=255)                        ctctTCTTCGAAGAAAGATCGCGGTAAAGGCTCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCTGCACTGGTGTTAGCGTAACCCGCAGCGGGTATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAACCCGGTGGGCGCTAACACCCTGACCGGCGTGGc                                                                                                                                                                                                              <  1:297623/249‑1 (MQ=255)                            tcttcGAAGAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAAt                                                                                                                                                                                                           <  1:223106/248‑1 (MQ=255)                               tcGAAGAAAGATCGCGGTAAAGGCGCTCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTGGGTTAGTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAGGCGCGGCACTGGTGTTAGCGTAACCCGCAGCTGGGAGAACATTAGCCTCAAGATGCCGAACAATGTGACCTTCGACAGTAGCAGCGCGACCCTGAAACCGGTGGGGGCTTACACCCTGACTGGCGCGGCAATGGTa                                                                                                                                                                                                       <  2:191129/249‑1 (MQ=255)                                           cgcgGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCCGCTCTGGGCGGCGGCGTTGGTTATTACCTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAACCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATcc                                                                                                                                                                                         >  1:194965/1‑251 (MQ=255)                                               gTAAAGCCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGaa                                                                                                                                                                                      >  1:119388/1‑250 (MQ=255)                                                         gcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGAGGGCGCTAACACCCTGACCGGCGTGGCAATGGTAATGAAAGAGTATCCGAAAAcggcgggtg                                                                                                                                                                           >  2:62799/1‑248 (MQ=255)                                                                      gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTaaaaagaatggttt                                                                                                                                                              >  1:6062/1‑239 (MQ=255)                                                                        agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACACGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGTTAACACCCTGACCGGCGTGGCAATGGTACTGAACGAGTATCCGAAAACGGCGGTTAACgggggtgggtatt                                                                                                                                                            >  2:1048/1‑239 (MQ=255)                                                                                       gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACgg                                                                                                                                                <  1:280011/248‑1 (MQ=255)                                                                                            gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCGGCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGTTGGTCAc                                                                                                                                        <  2:19491/251‑1 (MQ=255)                                                                                              ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTTACGTGATTGGTTTTAACGACAGCACgggggggcac                                                                                                                                        >  1:207677/1‑242 (MQ=255)                                                                                                            cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATATCATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAATCCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGtc                                                                                                                        <  1:301888/251‑1 (MQ=255)                                                                                                                     gCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTATCACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAg                                                                                                                <  1:212952/250‑1 (MQ=255)                                                                                                                            gcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGc                                                                                                        <  1:122681/251‑1 (MQ=255)                                                                                                                              gaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGGGATTGGGTATACCGAAAGCACGGGGGGGCACGACCTGAACATGCGTCTCTCCCAGCAAAGTGCgg                                                                                                      >  1:165157/1‑251 (MQ=255)                                                                                                                                        gACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGAGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTTACGTGATTTGTTATCCACCCAGCAAAGGGGGTCATGACCCGAACACTCGTCTCTCCCAGCAAAGTGCGGAGTCCGtttg                                                                                            >  1:165772/1‑249 (MQ=255)                                                                                                                                          cAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCa                                                                                          <  1:163266/251‑1 (MQ=255)                                                                                                                                             aaTGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTTAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAgcg                                                                                       <  1:290409/251‑1 (MQ=255)                                                                                                                                                    ggCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGc                                                                                                        <  1:266810/227‑1 (MQ=255)                                                                                                                                                    ggCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAACGGGGTTGGTTTTACCGCAAGCACGGGGGGGCACGGCCTGAACATGCGGCTTTCCCAGCCACGTGcagaacgggagggaggggggggggg                                                                                >  2:266810/1‑227 (MQ=255)                                                                                                                                                             gtTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAgg                                                                        <  1:171638/250‑1 (MQ=255)                                                                                                                                                                aGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGGTATACCGCCAGCACGGGGGGGCACGACCCGAAAATGCGGCTCTCCCCGCAACGTGCGGATTCCGCTGCCACCGCGGTTATCACCCCGGGCgg                                                                    >  1:69827/1‑250 (MQ=255)                                                                                                                                                                   gTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGGCACGACCCGAACATGCGTCTCTCCCCGCAACGTGCGGATTCCGTTGCCCGCGCGGTGGTCACCCACGGCggggg                                                                 >  1:5936/1‑247 (MQ=255)                                                                                                                                                                     aaCCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCTCCCAGGGCATGGACg                                                               <  1:91780/251‑1 (MQ=255)                                                                                                                                                                     aaCCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGAc                                                                <  1:223981/250‑1 (MQ=255)                                                                                                                                                                                gggATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGTCCCTGAAACCGGTGGGCTCTAACACCCTGCCCGGCGTGGCAATGGTACTGAAAGAGTATCCTAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCCCCCAGGGCGTGGACGCCAGCCGCATc                                                    <  2:209444/251‑1 (MQ=255)                                                                                                                                                                                gggATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGAGATTTGTTTTACCGACAGCACGGGTGGGCACCACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTTCGTTGCCCGCGGGTTGCTCACCCACGGCGGGGGCGcccgacgcctc                                                    >  1:48493/1‑242 (MQ=255)                                                                                                                                                                                      aCATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTTTACAGAACGCACGGGGGGTCACGACCTGAACATGCGTATCTCCCAGCAAAGGGCGGATTCCGTTGCCAACGCGGTGGTCCCCCAGGGCGGGGAGTCCCCCCGCATCCGTAcc                                              >  1:178428/1‑250 (MQ=255)                                                                                                                                                                                      aCATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAAAGTGATTGGTTATACCCAAAGCCCGGGGGGGCACGACCCGGACAAGCGTCTCTCCCCGCAACGGGCGGGTTCCGTTTCCCACGCGGTGTTCACCCCGGGCGGGGACGCCCGCCGCATCCCTAcc                                              >  1:308962/1‑250 (MQ=255)                                                                                                                                                                                        aTTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACCCCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCa                                            <  2:165157/251‑1 (MQ=255)                                                                                                                                                                                                 aagaggccgaaaacggtgACCTTCGACAGAAGCAGAGCGACCCTGAAACCGGGGGGCGCTAAATCCCTGACCGGGGTGGCAAGGGTACTGAAATAGTTTCCGAAACCGGCGGTTAACGTGATTGGTTATACTGACAGCACGGGTGTTCACGCCCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCGCCCAGGGCGTGGACGCCAGCTGCATCCGTACTCAGGGCCTTgg                                   <  2:245152/236‑1 (MQ=255)                                                                                                                                                                                                            cAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAAcc                        <  1:186057/251‑1 (MQ=255)                                                                                                                                                                                                             aaTGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAAGGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCTCGACCTGAACATGCGTCTCTccc                                                                                                                  <  2:132475/160‑1 (MQ=255)                                                                                                                                                                                                                           cagcagcagCGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACa         <  2:5936/251‑1 (MQ=255)                                                                                                                                                                                                                                 cagcGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCg   <  1:170714/251‑1 (MQ=255)                                                                                                                                                                                                                                  agcGCGACCCTGAAACCGGTGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGc  <  1:12153/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        GTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGAAGAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGC  >  NC_000913/3716679‑3717154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑