Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	49.1 / NA	16	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (5/11);  total (5/11)

GTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGC  >  NC_000913/3707730‑3708181                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           gttgttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgc                                                                                                                                                                                                          >  1:242329/1‑251 (MQ=255) gttgttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTGTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTg                                                                                                                                                                                                           <  1:41209/250‑1 (MQ=255)            ccTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAt                                                                                                                                                                                               >  1:133962/1‑251 (MQ=255)                tGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTGTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAAGCCACGAACAAAGATTAAAAAATCTTCAAACAGCTATTTGCAGCAGAGAAATTtg                                                                                                                                                                                            <  1:96455/250‑1 (MQ=255)                           gTCGCTGCCTGTCCTTTTTTGTGTTTTGCCCGGTCGTGTCAACGTTATGAGTTGGGTATGCCGTACTAATTAACATCAGTGTAACAGTGTGTACCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGTTTAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGTAGAGAAATTTGTGCTACTCCAAc                                                                                                                                                                                <  2:183774/251‑1 (MQ=255)                                               gttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCtt                                                                                                                                                            <  1:275677/251‑1 (MQ=255)                                                      gCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAttt                                                                                                                                                     <  2:133962/251‑1 (MQ=255)                                                                            aGGTGGGGATTCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGtt                                                                                                                               <  1:124067/251‑1 (MQ=255)                                                                                                                           tccATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGCAAAGGTGGATATCTGAGc                                                                                >  2:235364/1‑251 (MQ=255)                                                                                                                            ccATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCATCATGACCAGAACAATCAGCTTAATATTTAGCAACATGTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGAGATCTGAg                                                                                 <  1:225678/249‑1 (MQ=255)                                                                                                                                        ccTCGTGACCTACAATCTATCAACAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATg                                                                   <  1:169151/251‑1 (MQ=255)                                                                                                                                                              aCAGAATGTGAAA‑CGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGa                                              >  1:33032/1‑250 (MQ=255)                                                                                                                                                                            agTCAATTCAGCCAACCCGGATTTTCACCAACGGTGAGAATCCACGAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTATCAACATTTGGTGATTAATTTTGATGCATTTAATGAAAAAAATCTTAGGAAAAGGTGTATATCTTAGCTATTAATACCATGGACGCTAAAACGCACAGCGGAAAATGCTAGGgcca                                <  2:40769/250‑1 (MQ=255)                                                                                                                                                                                          aCCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGc                  <  2:242329/251‑1 (MQ=255)                                                                                                                                                                                                     cacCATCGGTGATAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCATCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATTAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTAATTTATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGAGTAATGCCATGATCGCTAAAACGCACATGGGAAAATTCCAGGGCCAGCCATAAGTAACGCAAAAAAAGAtt       <  1:45843/251‑1 (MQ=255)                                                                                                                                                                                                          aCGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGATACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATATGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGc  >  1:12674/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           GTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGC  >  NC_000913/3707730‑3708181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑