| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,635,603 | G→A | A255T (GCT→ACT) | yhiM → | inner membrane protein YhiM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,635,603 | 0 | G | A | 96.3% | 87.5 / NA | 27 | A255T (GCT→ACT) | yhiM | inner membrane protein YhiM |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/12); minor base T (0/1); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTCTATTACGCTGTTACGTAGTGCTGACATAACTCCGCATTATGTCGCGGGTCACGTATTGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCGTCTTCACCTGCCTGTTTTGCCTTTTCCTGGCATCGTTTCTTGCGGAAATGGCGCAGACCGACATGGGATATTTTATTCCTTCGCGAGTTCTGGTCGGTTTGGGAGCGGTATGCTTTA > NC_000913/3635357‑3635843 | tGGTCTATTGCGCTGTTACGTAGTGCTGACATAACTCCGCATTATGTCGCGGGTCACGTATTGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCGCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTc < 2:256670/250‑1 (MQ=255) cTGTTACGTAGTGCTGACATAACTCCGCATTATGTCGCGGGTCACGTATAGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGAGTTATTGGGTTATTTTTATCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCt > 1:50452/1‑251 (MQ=255) gTGCTGACATAACTCCGCATTATGACGCGGGTCACGTATCGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTc > 1:116406/1‑251 (MQ=255) gCTGACATAACTCCGCATTATGTCGCGGGTCACGTATTGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCg < 1:246599/250‑1 (MQ=255) gACATAACTCCGCATTATGTCGCGGGTCACGTATTGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCAAGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAAt > 2:101579/1‑251 (MQ=255) aTAACTCCGCATTATGTCGCGGGTCACGTATTGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGAt < 1:82200/251‑1 (MQ=255) aTGTCGCGGGTCACGTATTGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCata < 1:147759/251‑1 (MQ=255) cgcgGGTCACGTATTGCTTGGGTTACCCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGAGTTCTTGGGTTATTTTTCTCGGCTCAATCAAGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATtct > 2:291658/1‑251 (MQ=255) tAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACt < 1:66631/250‑1 (MQ=255) cGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTAt > 2:240072/1‑251 (MQ=255) gtgCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGGACTGCAGGGGTTATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAACTTATGGCAACTGGCACTGGTATGGAGACGt > 2:78459/1‑251 (MQ=255) tCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGtt > 1:217171/1‑251 (MQ=255) ccTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCATTTCCGATGCAAGCGCCCGACTGTCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGTCACTGGTATGGAGACGTACCTGTTCGTTAGCCa < 1:126352/251‑1 (MQ=255) ccTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATtctc > 1:254100/1‑201 (MQ=255) ccTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATtctc < 2:254100/201‑1 (MQ=255) ccTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCaa > 1:231674/1‑203 (MQ=255) tgCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGa < 1:271924/250‑1 (MQ=255) ccACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCGCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTAGTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCGACTGGCACTGGTATGGAGACTTACCTGTTCGTTAGCCATCAGAAt < 2:302336/250‑1 (MQ=255) cAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCg < 1:240072/250‑1 (MQ=255) ttCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCGTCTTCACCTGCCTGtttt > 2:6075/1‑251 (MQ=255) aCTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGTCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCgat < 2:26253/221‑1 (MQ=255) aCTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCgat > 1:26253/1‑221 (MQ=255) cacTGGCTGGGGGGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGCCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCGTCTTCACCTGCCTGTTTTGCCTTTTCCTGGc > 2:293517/1‑251 (MQ=255) aGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCGTCTTCACCTGCCTGTTTTGCCTTTTCCTGGCATCGTTTCTTGCGGAAATGGCGCAGACCGACATGGGATCTTTTATTCCTTcg > 2:149423/1‑251 (MQ=255) aCGTCTAAATCAGTTCCGATGCAAGCGCACGACTGACTCCCGCCATTATTCTTATTTGGCTCGGAATGATCTGTTACAGCATATACTCAAAAGTCTGGCTACTGACACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGCATACCGATGCTTCCCGTCTTCACCTGCCTGTTTTGCCTTTTCCTGGCATAGTTTCTTGCGGAACTGGCGCAGACCGACATGGGATATTTTATTCCTTCGCGAGTTCTg > 2:257574/1‑251 (MQ=255) ccGATGCAAGCGCCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCGTCTTCACCTGCCTGTTTTGCCTTTTCCTGGCATCGTTTCTTGCGGAAATGGCGCAGACCGACATGGGATATTTTATTCCTTCGCGAGTTCTGGTCGGTTTGGGAGc < 1:149423/250‑1 (MQ=255) cgcCCGACTGACTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCGTCTTCACCTGCCTGTTTTGCCTTTTCCTGGCATCGTTTCTTGCGGAAATGGCGCAGACCGACATGGGATATTTTATTCCTTCGCGAGTTCTGGTCGGTTTGGGAGCGGTATGCTTTa < 1:6075/251‑1 (MQ=255) | TGGTCTATTACGCTGTTACGTAGTGCTGACATAACTCCGCATTATGTCGCGGGTCACGTATTGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGGAGACGTACCTGTTCGTTAGCCAACAGAATACCGATGATTCCCGTCTTCACCTGCCTGTTTTGCCTTTTCCTGGCATCGTTTCTTGCGGAAATGGCGCAGACCGACATGGGATATTTTATTCCTTCGCGAGTTCTGGTCGGTTTGGGAGCGGTATGCTTTA > NC_000913/3635357‑3635843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |